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The map label for this gene is trmB [H]

Identifier: 21244028

GI number: 21244028

Start: 3881164

End: 3881949

Strand: Direct

Name: trmB [H]

Synonym: XAC3303

Alternate gene names: 21244028

Gene position: 3881164-3881949 (Clockwise)

Preceding gene: 21244027

Following gene: 21244029

Centisome position: 74.99

GC content: 65.52

Gene sequence:

>786_bases
ATGACTGATCCTTTCACAAGCGACGGCGCCAAGATGCCGCCCAAGCCCTTCACCATCGAAGAGGGGCGCCGGCAGGTGCG
CAGCTTCGTGCTGCGCCAGGGCCGCTTCACGCCGGCGCAGCAACGTACGTTCGATGCGCTATGGCCACGTTTCGGGCTGG
ATTACCTCGGTGCGCCACGCGATCTGGCTGCCACCTTCGGACGCGATGCGCACAAGGTGCTGGAAATCGGCTTCGGCAAT
GGCGCTGCGTTGCGCTTTGCCGCGCAGCAGGACCCAAGCCGCGATTACATCGGCATCGAGGTGCATGCCCCCGGCGTAGG
CCGCCTGCTCAACGCGCTGGAGGAAGACGGCAGCACCCATGTGCGCCTGTATCACCACGATGCGGTGGAAGTGCTGGAAC
ACGAGATCGCCGACGGTGCGCTGGACGAAGTGCGTATCTATTTCCCCGATCCATGGCACAAGAAGCGGCACAACAAGCGC
CGCCTGATCCAGCCGGCGTTCGCGCAGTTGCTGGTACGCAAGCTGCGCGAGGGCGGCCGCTTGCATGCGGCCACCGACTG
GGCCGATTACGCCGAACAGATGTGGGACGTGCTCGATGCCACCCCGGGCCTGGTCAATCGCGCCGGCCCCCGCGGCCACG
TCGAACGCCCCACCTGGCGCCCGCAAACCCACTTCGAGACGCGCGGCCAGAAACTCGGCCACGGCGTGTGGGATCTGCTG
TACGACCGGGATTCGGGAATCGGGAATCGGGAATCGCAAGAGCAACTGCCTTCGGCATCCGGATAG

Upstream 100 bases:

>100_bases
TGCGTCGCGGGCGCTCCCAGGCACGCGCGCCCATGGCGCGCAAACCGTGCCTCCTCGCCCCGGTCGGAGAGGGGCTTTCG
TTTCGCTGATGACCTAATTC

Downstream 100 bases:

>100_bases
CGTCTCCTTGCGTCGGCATTACCCGCTTTTCCCATTCTCCATTCCCGACTCCCTATTCCCGGCCCCCAATGGACACCGCG
CTGACGCTGACCAACGATAT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRTFDALWPRFGLDYLGAPRDLAATFGRDAHKVLEIGFGN
GAALRFAAQQDPSRDYIGIEVHAPGVGRLLNALEEDGSTHVRLYHHDAVEVLEHEIADGALDEVRIYFPDPWHKKRHNKR
RLIQPAFAQLLVRKLREGGRLHAATDWADYAEQMWDVLDATPGLVNRAGPRGHVERPTWRPQTHFETRGQKLGHGVWDLL
YDRDSGIGNRESQEQLPSASG

Sequences:

>Translated_261_residues
MTDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRTFDALWPRFGLDYLGAPRDLAATFGRDAHKVLEIGFGN
GAALRFAAQQDPSRDYIGIEVHAPGVGRLLNALEEDGSTHVRLYHHDAVEVLEHEIADGALDEVRIYFPDPWHKKRHNKR
RLIQPAFAQLLVRKLREGGRLHAATDWADYAEQMWDVLDATPGLVNRAGPRGHVERPTWRPQTHFETRGQKLGHGVWDLL
YDRDSGIGNRESQEQLPSASG
>Mature_260_residues
TDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRTFDALWPRFGLDYLGAPRDLAATFGRDAHKVLEIGFGNG
AALRFAAQQDPSRDYIGIEVHAPGVGRLLNALEEDGSTHVRLYHHDAVEVLEHEIADGALDEVRIYFPDPWHKKRHNKRR
LIQPAFAQLLVRKLREGGRLHAATDWADYAEQMWDVLDATPGLVNRAGPRGHVERPTWRPQTHFETRGQKLGHGVWDLLY
DRDSGIGNRESQEQLPSASG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=226, Percent_Identity=49.1150442477876, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 29539; Mature: 29408

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRTFDALWPRFGLDYLGAPR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHCCCCH
DLAATFGRDAHKVLEIGFGNGAALRFAAQQDPSRDYIGIEVHAPGVGRLLNALEEDGSTH
HHHHHHCCCHHHHEEEECCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCE
VRLYHHDAVEVLEHEIADGALDEVRIYFPDPWHKKRHNKRRLIQPAFAQLLVRKLREGGR
EEEEEHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
LHAATDWADYAEQMWDVLDATPGLVNRAGPRGHVERPTWRPQTHFETRGQKLGHGVWDLL
EEEECCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YDRDSGIGNRESQEQLPSASG
HCCCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure 
TDPFTSDGAKMPPKPFTIEEGRRQVRSFVLRQGRFTPAQQRTFDALWPRFGLDYLGAPR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHCCCCH
DLAATFGRDAHKVLEIGFGNGAALRFAAQQDPSRDYIGIEVHAPGVGRLLNALEEDGSTH
HHHHHHCCCHHHHEEEECCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCE
VRLYHHDAVEVLEHEIADGALDEVRIYFPDPWHKKRHNKRRLIQPAFAQLLVRKLREGGR
EEEEEHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
LHAATDWADYAEQMWDVLDATPGLVNRAGPRGHVERPTWRPQTHFETRGQKLGHGVWDLL
EEEECCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YDRDSGIGNRESQEQLPSASG
HCCCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA