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The map label for this gene is glgX [H]

Identifier: 21243980

GI number: 21243980

Start: 3834339

End: 3836471

Strand: Direct

Name: glgX [H]

Synonym: XAC3254

Alternate gene names: 21243980

Gene position: 3834339-3836471 (Clockwise)

Preceding gene: 21243974

Following gene: 21243985

Centisome position: 74.09

GC content: 61.79

Gene sequence:

>2133_bases
ATGCGACGTCCAGCAAGCGCGGCCTATGCCAACCCCTCCCGCATCCGTCAGGGGCGCCCGTTCCCACGCGGTGCCGTCTT
CGACGGCAAGGGCACCAATTTCGCGCTGTTTTCCGCGCATGCCACGCGTGTCGAGCTGTGCCTGTTCGACGAACAGGGCA
ACGAAACCCGCGTCGACCTGCCCGAGTACACCAACGAGATCTGGCACGGGTATCTTCCCGATGCCAAGCCGGGCCAGCGC
TATGGCTACCGTGTTCACGGCCCCTATGCGCCGACCGAAGGCCATCGCTTTAATCACAACAAGCTGCTATTGGATCCGTA
TGCGCGCGAGCTCGACGGCGATCTGATCTGGTCCGATGAGCTCTACGGCTACACCGTGGGCCACCCCGACGGCGACCTGA
GCTTCGACGAGCGCGATAGCGCGCCCTTCATGCCCAAGTGCGTGGTGGTCGAAGACACCTACGACTGGGGAGACGACACC
CGCCTGCTCAAGCCGTGGAATGAAACGGTCATCTACGAAACCCATGTGCGCGGCTACACGATGCGCAATCCACAGGTACC
CGAAGCGGTGCGCGGCACTTTCGCCGGCCTGGCCCAGCCGCAGGTACTCCAGTACATCAAGGACCTGGGCATCACCGCGG
TGGAGCTTTTGCCGGTACACGCGTACCTGGACGACCAGCATCTGCTCGACAAGGGGCTACGTAATTACTGGGGCTACAAC
ACCATCGGCTTTTTCGCGCTGAAGTCGCGCTACCTGTCCAGCGGACATCGCGATGAGTTCCGCGACATGGTCAAGGCCAT
GCACAAACAGGGTCTGGAAGTGATTCTGGACGTGGTCTACAACCACACCGCCGAAGGCAGCGAGCTGGGGCCGACCTTGT
CGTTCAAGGGCATCGACAACGCCAGCTATTACCGCCTGGCCGATGACAAGCGCTACTACATCAACGATACCGGTACCGGA
AATACGCTGAACCTGAGCAATTCGCGGGTGATCCAGTTCGTCAACGATTCGCTGCGCTACTGGGCCGGCGAAATGCATGT
GGATGGCTTTCGCTTCGATCTGGCCACCATTCTTGGCCGCGAGCCGAGTGGCTTCGATCAACGTGGCGGCTTCCTGGATG
CCTGCAACCAGGACCCGCTGCTGTCGGAAGTCAAGCTGATTGCCGAGCCGTGGGACTGCGGCCCAGGCGGTTATCAGGTA
GGCCACTTCCCGCCGGGTTGGTCGGAGTGGAACGACAAGTTCCGTGACAATGCGCGCGAATTCTGGAAAGGCGAAGACGG
CAAGCTGGCGGAATTCGCGACCCGCTTCACCGGTTCGGCCGATCTATTCGATCGCCGCGGCCGGCGCCCGTGGGCGTCGG
TGAACTTCATCACCGCCCACGATGGCTTCACCTTGCGCGATCTGGTCAGCTACAACGAAAAGCACAATATCGATAACGGC
GAGGACAACCGCGACGGTTCGTCGAACGATGGCTCCTGCAACTACGGCGAAGAAGGCGACACCGATAACGCCGAGGTGCT
GCAGATCCGCGAGCGGCAGATGAAGAACCTGCTTGCCACGCTGCTGCTATCGCAAGGCACGCCGATGATGCTCAGCGGCG
ACGAGCGTGCGCAGACACAGGGGGGCAACAACAATACCTATTGCCAGGACAACGAAATCACCTGGCTGGACTGGGAAAAC
GATCCGACCGACGGCCGCCTGACCGAGTTCGTGAAGGCACTGACCCGTCTGCGCAAGCGTTATCCGATCCTGTCGCGGGG
CCGCTTTCTCAACGGCCAGTACAACGAAGAAGCCGGCCTGCGCGATCTCACCTGGCTCAATCCAGGCGGCATCGAAATGG
ACGATGCGCACTGGACCGACCCCGCCGCGCGCTCGGTGGGCCTGTTGCTGGAAGGCAAGGCGCAGACCTCGGGGGTCAAG
GAACTGGCCAACGACGACACCTTGCTCATCGTCATCAACGCCTATCACGAGGGCGTGACCTTTACCCTGCCCTCCTCCGA
TGAGCCCGTGCACTGGAAGCTGGTGCTCTCCACCGACGAAGCGCTGGAGGTGGACATGATGCCGGCCGGTGCCAGCGAAT
TCCTGGCCCCGCCGCGTAGCGTGTCGGTCTTCGAGTGCAAGAGCGACCAGTAA

Upstream 100 bases:

>100_bases
CTCACGCCACACCTCTGTTGCCGTAACGCGTCTTGCACAGCTGTAGACGCAATGCTTGAGGCCCCTCAATGCCGGCGTGC
CGGCGCATACGAGACTTTCC

Downstream 100 bases:

>100_bases
AGCTGTCCCAGCAACGCGCACTCAGGAGCGACGCTTGCTGTCTACCGGTATCGGGTAGGCAGCAAGCGTTTTTTGTTTCG
GTCGATGGAATGGCGGATTG

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MRRPASAAYANPSRIRQGRPFPRGAVFDGKGTNFALFSAHATRVELCLFDEQGNETRVDLPEYTNEIWHGYLPDAKPGQR
YGYRVHGPYAPTEGHRFNHNKLLLDPYARELDGDLIWSDELYGYTVGHPDGDLSFDERDSAPFMPKCVVVEDTYDWGDDT
RLLKPWNETVIYETHVRGYTMRNPQVPEAVRGTFAGLAQPQVLQYIKDLGITAVELLPVHAYLDDQHLLDKGLRNYWGYN
TIGFFALKSRYLSSGHRDEFRDMVKAMHKQGLEVILDVVYNHTAEGSELGPTLSFKGIDNASYYRLADDKRYYINDTGTG
NTLNLSNSRVIQFVNDSLRYWAGEMHVDGFRFDLATILGREPSGFDQRGGFLDACNQDPLLSEVKLIAEPWDCGPGGYQV
GHFPPGWSEWNDKFRDNAREFWKGEDGKLAEFATRFTGSADLFDRRGRRPWASVNFITAHDGFTLRDLVSYNEKHNIDNG
EDNRDGSSNDGSCNYGEEGDTDNAEVLQIRERQMKNLLATLLLSQGTPMMLSGDERAQTQGGNNNTYCQDNEITWLDWEN
DPTDGRLTEFVKALTRLRKRYPILSRGRFLNGQYNEEAGLRDLTWLNPGGIEMDDAHWTDPAARSVGLLLEGKAQTSGVK
ELANDDTLLIVINAYHEGVTFTLPSSDEPVHWKLVLSTDEALEVDMMPAGASEFLAPPRSVSVFECKSDQ

Sequences:

>Translated_710_residues
MRRPASAAYANPSRIRQGRPFPRGAVFDGKGTNFALFSAHATRVELCLFDEQGNETRVDLPEYTNEIWHGYLPDAKPGQR
YGYRVHGPYAPTEGHRFNHNKLLLDPYARELDGDLIWSDELYGYTVGHPDGDLSFDERDSAPFMPKCVVVEDTYDWGDDT
RLLKPWNETVIYETHVRGYTMRNPQVPEAVRGTFAGLAQPQVLQYIKDLGITAVELLPVHAYLDDQHLLDKGLRNYWGYN
TIGFFALKSRYLSSGHRDEFRDMVKAMHKQGLEVILDVVYNHTAEGSELGPTLSFKGIDNASYYRLADDKRYYINDTGTG
NTLNLSNSRVIQFVNDSLRYWAGEMHVDGFRFDLATILGREPSGFDQRGGFLDACNQDPLLSEVKLIAEPWDCGPGGYQV
GHFPPGWSEWNDKFRDNAREFWKGEDGKLAEFATRFTGSADLFDRRGRRPWASVNFITAHDGFTLRDLVSYNEKHNIDNG
EDNRDGSSNDGSCNYGEEGDTDNAEVLQIRERQMKNLLATLLLSQGTPMMLSGDERAQTQGGNNNTYCQDNEITWLDWEN
DPTDGRLTEFVKALTRLRKRYPILSRGRFLNGQYNEEAGLRDLTWLNPGGIEMDDAHWTDPAARSVGLLLEGKAQTSGVK
ELANDDTLLIVINAYHEGVTFTLPSSDEPVHWKLVLSTDEALEVDMMPAGASEFLAPPRSVSVFECKSDQ
>Mature_710_residues
MRRPASAAYANPSRIRQGRPFPRGAVFDGKGTNFALFSAHATRVELCLFDEQGNETRVDLPEYTNEIWHGYLPDAKPGQR
YGYRVHGPYAPTEGHRFNHNKLLLDPYARELDGDLIWSDELYGYTVGHPDGDLSFDERDSAPFMPKCVVVEDTYDWGDDT
RLLKPWNETVIYETHVRGYTMRNPQVPEAVRGTFAGLAQPQVLQYIKDLGITAVELLPVHAYLDDQHLLDKGLRNYWGYN
TIGFFALKSRYLSSGHRDEFRDMVKAMHKQGLEVILDVVYNHTAEGSELGPTLSFKGIDNASYYRLADDKRYYINDTGTG
NTLNLSNSRVIQFVNDSLRYWAGEMHVDGFRFDLATILGREPSGFDQRGGFLDACNQDPLLSEVKLIAEPWDCGPGGYQV
GHFPPGWSEWNDKFRDNAREFWKGEDGKLAEFATRFTGSADLFDRRGRRPWASVNFITAHDGFTLRDLVSYNEKHNIDNG
EDNRDGSSNDGSCNYGEEGDTDNAEVLQIRERQMKNLLATLLLSQGTPMMLSGDERAQTQGGNNNTYCQDNEITWLDWEN
DPTDGRLTEFVKALTRLRKRYPILSRGRFLNGQYNEEAGLRDLTWLNPGGIEMDDAHWTDPAARSVGLLLEGKAQTSGVK
ELANDDTLLIVINAYHEGVTFTLPSSDEPVHWKLVLSTDEALEVDMMPAGASEFLAPPRSVSVFECKSDQ

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=199, Percent_Identity=29.1457286432161, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI2367229, Length=657, Percent_Identity=49.7716894977169, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1789839, Length=343, Percent_Identity=26.2390670553936, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80250; Mature: 80250

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPASAAYANPSRIRQGRPFPRGAVFDGKGTNFALFSAHATRVELCLFDEQGNETRVDL
CCCCCCCCCCCHHHHHCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEEECCCCCEEEEC
PEYTNEIWHGYLPDAKPGQRYGYRVHGPYAPTEGHRFNHNKLLLDPYARELDGDLIWSDE
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCHHHHCCCCEEECCC
LYGYTVGHPDGDLSFDERDSAPFMPKCVVVEDTYDWGDDTRLLKPWNETVIYETHVRGYT
CCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCCEEEEEEECCCEE
MRNPQVPEAVRGTFAGLAQPQVLQYIKDLGITAVELLPVHAYLDDQHLLDKGLRNYWGYN
CCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHCCCC
TIGFFALKSRYLSSGHRDEFRDMVKAMHKQGLEVILDVVYNHTAEGSELGPTLSFKGIDN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCC
ASYYRLADDKRYYINDTGTGNTLNLSNSRVIQFVNDSLRYWAGEMHVDGFRFDLATILGR
CCEEEEECCCEEEEECCCCCCEEECCCCCEEEEHHHHHHHHCCCEECCCHHHHHHHHHCC
EPSGFDQRGGFLDACNQDPLLSEVKLIAEPWDCGPGGYQVGHFPPGWSEWNDKFRDNARE
CCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FWKGEDGKLAEFATRFTGSADLFDRRGRRPWASVNFITAHDGFTLRDLVSYNEKHNIDNG
HHCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHCCHHCCCCCC
EDNRDGSSNDGSCNYGEEGDTDNAEVLQIRERQMKNLLATLLLSQGTPMMLSGDERAQTQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCC
GGNNNTYCQDNEITWLDWENDPTDGRLTEFVKALTRLRKRYPILSRGRFLNGQYNEEAGL
CCCCCCEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC
RDLTWLNPGGIEMDDAHWTDPAARSVGLLLEGKAQTSGVKELANDDTLLIVINAYHEGVT
CEEEEECCCCCCCCCCCCCCHHHHCEEEEEECCCHHHHHHHHCCCCEEEEEEEECCCCEE
FTLPSSDEPVHWKLVLSTDEALEVDMMPAGASEFLAPPRSVSVFECKSDQ
EECCCCCCCEEEEEEEECCCCEEEEECCCCHHHHCCCCCCCEEEECCCCC
>Mature Secondary Structure
MRRPASAAYANPSRIRQGRPFPRGAVFDGKGTNFALFSAHATRVELCLFDEQGNETRVDL
CCCCCCCCCCCHHHHHCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEEECCCCCEEEEC
PEYTNEIWHGYLPDAKPGQRYGYRVHGPYAPTEGHRFNHNKLLLDPYARELDGDLIWSDE
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCHHHHCCCCEEECCC
LYGYTVGHPDGDLSFDERDSAPFMPKCVVVEDTYDWGDDTRLLKPWNETVIYETHVRGYT
CCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCCEEEEEEECCCEE
MRNPQVPEAVRGTFAGLAQPQVLQYIKDLGITAVELLPVHAYLDDQHLLDKGLRNYWGYN
CCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHCCCC
TIGFFALKSRYLSSGHRDEFRDMVKAMHKQGLEVILDVVYNHTAEGSELGPTLSFKGIDN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCC
ASYYRLADDKRYYINDTGTGNTLNLSNSRVIQFVNDSLRYWAGEMHVDGFRFDLATILGR
CCEEEEECCCEEEEECCCCCCEEECCCCCEEEEHHHHHHHHCCCEECCCHHHHHHHHHCC
EPSGFDQRGGFLDACNQDPLLSEVKLIAEPWDCGPGGYQVGHFPPGWSEWNDKFRDNARE
CCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FWKGEDGKLAEFATRFTGSADLFDRRGRRPWASVNFITAHDGFTLRDLVSYNEKHNIDNG
HHCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHCCHHCCCCCC
EDNRDGSSNDGSCNYGEEGDTDNAEVLQIRERQMKNLLATLLLSQGTPMMLSGDERAQTQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCC
GGNNNTYCQDNEITWLDWENDPTDGRLTEFVKALTRLRKRYPILSRGRFLNGQYNEEAGL
CCCCCCEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC
RDLTWLNPGGIEMDDAHWTDPAARSVGLLLEGKAQTSGVKELANDDTLLIVINAYHEGVT
CEEEEECCCCCCCCCCCCCCHHHHCEEEEEECCCHHHHHHHHCCCCEEEEEEEECCCCEE
FTLPSSDEPVHWKLVLSTDEALEVDMMPAGASEFLAPPRSVSVFECKSDQ
EECCCCCCCEEEEEEEECCCCEEEEECCCCHHHHCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]