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The map label for this gene is mphE [H]

Identifier: 21243901

GI number: 21243901

Start: 3734499

End: 3735275

Strand: Direct

Name: mphE [H]

Synonym: XAC3175

Alternate gene names: 21243901

Gene position: 3734499-3735275 (Clockwise)

Preceding gene: 21243897

Following gene: 21243902

Centisome position: 72.16

GC content: 67.7

Gene sequence:

>777_bases
TTGGCGGCCTCTTCCATGGCGGTTGCAGTCGGCATCCTCAATGCAGTGCCAAGCGTGCAGCTCTGCGAATTGCTGGGTCG
CATCGGCTATGGCTTTGTGGTGCTCGATCTGGAGCATGTGTTGCGTGCGCCCGACACGCTGGAACATGCGATCCGCGCCT
GCGAGTTGTCGGGTTGCGAGGCCTGGGTGCGCGTGCCGGAAGTGGACGAAAAACTGATTGGCCGGGTGCTGGATGCCGGC
GCGCGCGGGATCGTGATTCCGCGTACCGAGTCTGCGGCGCAGATCGGCGACGCCATCGCCGCGGCGCGCTTCCCGCCGCT
GGGCCGGCGCGGCATCACCGGCGGCCGTGTCACCGGCTTCGGCACTGTCGATCTGCCCACCTACATCGCGCAGGCCAATG
CGAACATCCGTCTCGTGCCGATGATCGAAAGCGCCGCCGGCGTGGCTGCCCTGCCGGAGATTCTTGCCACCCCCGGCGTG
GCCCTGGTGATGGAAGGTGCGCTGGATCTGGCGCTGGATCTTGGTCTGGGCCCGCAGCCCACCCACCCGCAGGTGTGGGA
ATTGCTGCTGCAATTGCACGCGCAGTGCCTTGCTGCAAACGTCCCGTTCTGCCCCAACCCGCGCACCGACGCGCAACGTG
CGCACTGGCTGCAACAGCCGGAGCTGCGCTGGCTGCTGGCCGGCGAAGACCGCGCGCTGATCCAACGCGCCTTGCGCGGC
CATTTGGCCAGCCTTGCCGTCCCCCATTCACCTCCCGCCTGCAGGAGCACGCCGTAG

Upstream 100 bases:

>100_bases
AAGCTAACCCATCGGAATCAAGCCGCAGTGGTTACCCAAAAAGACCGCCAGACACACTTGATAATCATTCCCATTAAGCG
CAGGATGAGCGCCCGTTTCC

Downstream 100 bases:

>100_bases
ATGTCGACGTTCCGTTTCCACGCCCTGGCCATCGCGATCGCGGCGAGCACGCTGACGCTGCCGCAGCTGACGCGCGCAAG
CGATACGCCGACGACCGATG

Product: 4-hydroxy-2-oxovalerate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAASSMAVAVGILNAVPSVQLCELLGRIGYGFVVLDLEHVLRAPDTLEHAIRACELSGCEAWVRVPEVDEKLIGRVLDAG
ARGIVIPRTESAAQIGDAIAAARFPPLGRRGITGGRVTGFGTVDLPTYIAQANANIRLVPMIESAAGVAALPEILATPGV
ALVMEGALDLALDLGLGPQPTHPQVWELLLQLHAQCLAANVPFCPNPRTDAQRAHWLQQPELRWLLAGEDRALIQRALRG
HLASLAVPHSPPACRSTP

Sequences:

>Translated_258_residues
MAASSMAVAVGILNAVPSVQLCELLGRIGYGFVVLDLEHVLRAPDTLEHAIRACELSGCEAWVRVPEVDEKLIGRVLDAG
ARGIVIPRTESAAQIGDAIAAARFPPLGRRGITGGRVTGFGTVDLPTYIAQANANIRLVPMIESAAGVAALPEILATPGV
ALVMEGALDLALDLGLGPQPTHPQVWELLLQLHAQCLAANVPFCPNPRTDAQRAHWLQQPELRWLLAGEDRALIQRALRG
HLASLAVPHSPPACRSTP
>Mature_257_residues
AASSMAVAVGILNAVPSVQLCELLGRIGYGFVVLDLEHVLRAPDTLEHAIRACELSGCEAWVRVPEVDEKLIGRVLDAGA
RGIVIPRTESAAQIGDAIAAARFPPLGRRGITGGRVTGFGTVDLPTYIAQANANIRLVPMIESAAGVAALPEILATPGVA
LVMEGALDLALDLGLGPQPTHPQVWELLLQLHAQCLAANVPFCPNPRTDAQRAHWLQQPELRWLLAGEDRALIQRALRGH
LASLAVPHSPPACRSTP

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=202, Percent_Identity=33.6633663366337, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788578, Length=184, Percent_Identity=29.3478260869565, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27304; Mature: 27173

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASSMAVAVGILNAVPSVQLCELLGRIGYGFVVLDLEHVLRAPDTLEHAIRACELSGCE
CCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHCCCCH
AWVRVPEVDEKLIGRVLDAGARGIVIPRTESAAQIGDAIAAARFPPLGRRGITGGRVTGF
HEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
GTVDLPTYIAQANANIRLVPMIESAAGVAALPEILATPGVALVMEGALDLALDLGLGPQP
ECCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCC
THPQVWELLLQLHAQCLAANVPFCPNPRTDAQRAHWLQQPELRWLLAGEDRALIQRALRG
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHH
HLASLAVPHSPPACRSTP
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AASSMAVAVGILNAVPSVQLCELLGRIGYGFVVLDLEHVLRAPDTLEHAIRACELSGCE
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHCCCCH
AWVRVPEVDEKLIGRVLDAGARGIVIPRTESAAQIGDAIAAARFPPLGRRGITGGRVTGF
HEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
GTVDLPTYIAQANANIRLVPMIESAAGVAALPEILATPGVALVMEGALDLALDLGLGPQP
ECCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCC
THPQVWELLLQLHAQCLAANVPFCPNPRTDAQRAHWLQQPELRWLLAGEDRALIQRALRG
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHH
HLASLAVPHSPPACRSTP
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA