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The map label for this gene is ruvC

Identifier: 21243876

GI number: 21243876

Start: 3700887

End: 3701411

Strand: Reverse

Name: ruvC

Synonym: XAC3150

Alternate gene names: 21243876

Gene position: 3701411-3700887 (Counterclockwise)

Preceding gene: 21243877

Following gene: 21243875

Centisome position: 71.52

GC content: 65.33

Gene sequence:

>525_bases
ATGACCCGCATCCTGGGCATCGATCCGGGTTCGCAGCGCACCGGCATCGGCATCATCGACATCGACGAGGGCGGTCGCAG
CCGGCATGTGCACCACGCGCCGCTGATGCTGCTGGGCGAGGGGGATTTCTCCCAGCGGCTCAAGCGCCTGCTGCAGGGCT
TGGGCGAGTTGATCGAGATCTATCGGCCGGATGAAGTCGCGATCGAAAAGGTGTTCATGGGCAAGAGTGCCGCTTCGGCG
CTCAAGCTCGGCCACGCGCGCGGCGCGGCGATCTGCGCGGTGGTCATGCGCGATCTGCCGGTGCACGAGTACGCGGCCAA
AGAAATCAAACTGGCGCTGGTCGGCAAGGGCGGCGCCGACAAGGTGCAGGTGCAGCACATGGTCGGCATCATGCTCAACT
TGAATGGCAAGCTGCAGCCCGACGCTGCCGACGCATTGGCGGTCGCCATCACTCACGCCCACGTGCGCGCCACCGCGCAA
CGCCTGGGCGTCAATACCCAACAGGCCTGGAGCCGCAAGAAATGA

Upstream 100 bases:

>100_bases
AATCGAAATCGCCGCATCCTGCATCGCTCGGCAGCATCGAGCATGCCGGTAGGCTGCTCCACCCATTCCCGATTCCCGAC
TTCCCATTCCCGGCCGCTCG

Downstream 100 bases:

>100_bases
GAATCGGGAATCGGCAATCGGGAATCGGGAATCCCAAACGCAGGGTCCACGACAGCGCGCGCTGTTGCGATTGGCCATTC
CCGATTCCCCATTCCCGGCA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIEIYRPDEVAIEKVFMGKSAASA
LKLGHARGAAICAVVMRDLPVHEYAAKEIKLALVGKGGADKVQVQHMVGIMLNLNGKLQPDAADALAVAITHAHVRATAQ
RLGVNTQQAWSRKK

Sequences:

>Translated_174_residues
MTRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIEIYRPDEVAIEKVFMGKSAASA
LKLGHARGAAICAVVMRDLPVHEYAAKEIKLALVGKGGADKVQVQHMVGIMLNLNGKLQPDAADALAVAITHAHVRATAQ
RLGVNTQQAWSRKK
>Mature_173_residues
TRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIEIYRPDEVAIEKVFMGKSAASAL
KLGHARGAAICAVVMRDLPVHEYAAKEIKLALVGKGGADKVQVQHMVGIMLNLNGKLQPDAADALAVAITHAHVRATAQR
LGVNTQQAWSRKK

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=170, Percent_Identity=50, Blast_Score=125, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_XANAC (Q8PHU9)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643458.1
- ProteinModelPortal:   Q8PHU9
- SMR:   Q8PHU9
- GeneID:   1157221
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3150
- NMPDR:   fig|190486.1.peg.3102
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- BioCyc:   XAXO190486:XAC3150-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18732; Mature: 18601

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIEI
CCEEEECCCCCCCCCEEEEEECCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
YRPDEVAIEKVFMGKSAASALKLGHARGAAICAVVMRDLPVHEYAAKEIKLALVGKGGAD
CCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHEEEEEEECCCCC
KVQVQHMVGIMLNLNGKLQPDAADALAVAITHAHVRATAQRLGVNTQQAWSRKK
CEEHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
TRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIEI
CEEEECCCCCCCCCEEEEEECCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
YRPDEVAIEKVFMGKSAASALKLGHARGAAICAVVMRDLPVHEYAAKEIKLALVGKGGAD
CCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHEEEEEEECCCCC
KVQVQHMVGIMLNLNGKLQPDAADALAVAITHAHVRATAQRLGVNTQQAWSRKK
CEEHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217