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The map label for this gene is exsB

Identifier: 21243864

GI number: 21243864

Start: 3689159

End: 3689833

Strand: Direct

Name: exsB

Synonym: XAC3137

Alternate gene names: 21243864

Gene position: 3689159-3689833 (Clockwise)

Preceding gene: 21243861

Following gene: 21243881

Centisome position: 71.28

GC content: 66.07

Gene sequence:

>675_bases
ATGAAAAAAGCCGTTGTTCTATTGTCCGGTGGCATGGATTCCGCCGCCGTCATCGCACTCGCGCAGGAGCAGGGCTTTGC
CGTCTACGCGCTGAGCGTTCGTTATGGCCAACGCCATACGTCGGAACTGGACGCTGCAGCGCGCGTTGCTGCCGCGCAGG
GCGTCATTGCGCACAAGGTGGTCGACGTGGACCTGCGCAGCATCGGCGGCTCGGCCTTGACCGACGACATCGAGGTCCCC
GACGCTGGCGGCGACGGTATCCCCGTTACGTATGTGCCCGCGCGCAACACCATCATGCTGTCGCTGGCGCTCGGTTGGGC
CGAGGTCATCGGCGCCAACGATCTGTTCTGTGGCGTCAATGCGGTGGACTACTCTGGCTACCCCGATTGCCGTCCCGAGT
TCGTGCGGGCTTTCGAGGTGCTGGCCAACCTGGCCACCAAGGCAGGTGTGGAAGGCGCGGGGCTGCGCGTGCACGCACCA
CTGCAGTTCCTCAGCAAGGCCGACATCGTCCGTGAAGGCGTGCGCCTGGGCGTGGACTTCGGCGTCACCGTCTCCTGCTA
CCGCGCAGATGCGGATGGGCGCGCCTGCGGCCACTGCGACGCCTGCCGCCTGCGCGCGGCCGGCTTCGCCGATGCGGGCA
TTCCCGACCCCACACACTACGCCATTTTGTCTTGA

Upstream 100 bases:

>100_bases
CCGCGGCAGCCAAACCTGGATCGATGACCGGCTCCTGGTCGCCCTGCACCATCGTCCCTGATAGCCTTCGGGCTTATTCC
GCAGTGATCGCCGATTCGCC

Downstream 100 bases:

>100_bases
CGCTCGCGTAGGGTAGAATGCGCACCCCGACGCAATGTCGGGTCAGTGGGCCGTTAGCTCAGTCGGTAGAGCAGAAGACT
TTTAATCTTTTGGTCGATGG

Product: transcriptional regulator

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKKAVVLLSGGMDSAAVIALAQEQGFAVYALSVRYGQRHTSELDAAARVAAAQGVIAHKVVDVDLRSIGGSALTDDIEVP
DAGGDGIPVTYVPARNTIMLSLALGWAEVIGANDLFCGVNAVDYSGYPDCRPEFVRAFEVLANLATKAGVEGAGLRVHAP
LQFLSKADIVREGVRLGVDFGVTVSCYRADADGRACGHCDACRLRAAGFADAGIPDPTHYAILS

Sequences:

>Translated_224_residues
MKKAVVLLSGGMDSAAVIALAQEQGFAVYALSVRYGQRHTSELDAAARVAAAQGVIAHKVVDVDLRSIGGSALTDDIEVP
DAGGDGIPVTYVPARNTIMLSLALGWAEVIGANDLFCGVNAVDYSGYPDCRPEFVRAFEVLANLATKAGVEGAGLRVHAP
LQFLSKADIVREGVRLGVDFGVTVSCYRADADGRACGHCDACRLRAAGFADAGIPDPTHYAILS
>Mature_224_residues
MKKAVVLLSGGMDSAAVIALAQEQGFAVYALSVRYGQRHTSELDAAARVAAAQGVIAHKVVDVDLRSIGGSALTDDIEVP
DAGGDGIPVTYVPARNTIMLSLALGWAEVIGANDLFCGVNAVDYSGYPDCRPEFVRAFEVLANLATKAGVEGAGLRVHAP
LQFLSKADIVREGVRLGVDFGVTVSCYRADADGRACGHCDACRLRAAGFADAGIPDPTHYAILS

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=225, Percent_Identity=40.4444444444444, Blast_Score=141, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_XANAC (Q8PHW1)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643446.1
- ProteinModelPortal:   Q8PHW1
- SMR:   Q8PHW1
- GeneID:   1157208
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3140
- NMPDR:   fig|190486.1.peg.3090
- HOGENOM:   HBG553284
- OMA:   GWAEVLG
- ProtClustDB:   CLSK446376
- BioCyc:   XAXO190486:XAC3137-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 23306; Mature: 23306

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAVVLLSGGMDSAAVIALAQEQGFAVYALSVRYGQRHTSELDAAARVAAAQGVIAHKV
CCCEEEEEECCCCCEEEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHEE
VDVDLRSIGGSALTDDIEVPDAGGDGIPVTYVPARNTIMLSLALGWAEVIGANDLFCGVN
EEEHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEHHHHHHHCCCCEEECCE
AVDYSGYPDCRPEFVRAFEVLANLATKAGVEGAGLRVHAPLQFLSKADIVREGVRLGVDF
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCC
GVTVSCYRADADGRACGHCDACRLRAAGFADAGIPDPTHYAILS
CCEEEEEEECCCCCCCCCCHHHHEEECCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MKKAVVLLSGGMDSAAVIALAQEQGFAVYALSVRYGQRHTSELDAAARVAAAQGVIAHKV
CCCEEEEEECCCCCEEEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHEE
VDVDLRSIGGSALTDDIEVPDAGGDGIPVTYVPARNTIMLSLALGWAEVIGANDLFCGVN
EEEHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEHHHHHHHCCCCEEECCE
AVDYSGYPDCRPEFVRAFEVLANLATKAGVEGAGLRVHAPLQFLSKADIVREGVRLGVDF
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCC
GVTVSCYRADADGRACGHCDACRLRAAGFADAGIPDPTHYAILS
CCEEEEEEECCCCCCCCCCHHHHEEECCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217