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The map label for this gene is rpoN [H]

Identifier: 21243699

GI number: 21243699

Start: 3474236

End: 3475675

Strand: Direct

Name: rpoN [H]

Synonym: XAC2972

Alternate gene names: 21243699

Gene position: 3474236-3475675 (Clockwise)

Preceding gene: 21243698

Following gene: 21243700

Centisome position: 67.13

GC content: 68.12

Gene sequence:

>1440_bases
ATGAAAGCCCGGCTCCAGACATCGCTAGGACAGCAGTTGGTCATGACGCCGCAGTTGCGTCAGGCGATCAAGCTATTGCA
GATGTCCACCACCGAGCTGGAAGTGGAAATCGCCGAGGCCGTGGAAACCAATCCGCTGCTGGAATGGGCCGACGAAGCCG
CGCATGCCGCCAGCGACATCGCGGTGGAATCCGCCCCGTCCGCGTCCACCGAAGAACCCCAGCGCGAAGAAGTCGCGCCT
GCCGAGCGCGACGACGATTGGTCGCAGGACGAGCTGCAGTGGACCGGCTCGGGCAGCGGCGGCAGCTTCGACGACGACGA
GTCCGGCGATGCCGCAGAGCGCGTGGCCGAATCCGAAACATTGGCCGACCACCTCTTGTGGCAGTTGCATCTGTCGCCGC
TGTCGCCGCGCGACCGCCAGATCGGCGCGATGCTGATCGACGCCCTCGACGAAGACGGCTACCTGCGCGAACCACTGTCG
GCCATTCTCGAAACCCTCGCGCTGGGCGCAGCGGTCGACGAGGCCGACGTGCTCACCGTCCTGCATCAGATCCAGCGCTT
CGACCCGGTGGGCGTGGGCGCGCGCACGCTGGGCGAATGCCTGGCACTGCAGCTGGGGGTGCTCGATGCGGACACGCCTG
GCCGCGAGCTGGCGCTGCAGATCGTTGCCGGCCCGCTGGAACGGCTGCCGCGCGCCGGCGTGGCCGGGCTGGCGCACGAG
CTCAAGCGCTCCACTGCCGATGTCGAACAGGCGGTGCAGCTGGTGCGCTCGCTGGACCCGCGCCCGGGTAAACAGATCGG
CGACCTGTCGCAGGACACCTACGTTGTTCCCGACTGCGTGATCTGGCGCCAGCGCGGGGTCTGGCGGGCTGCGCTGGCCG
GCCGCGCGCAGCCCAAGGTCACCATTCACCGCGGCTACGAAAACCTGATCCGCAGCTGCGGCGAATCCGATGCCGGCTAT
CTGCGCGGCCAGTTGCAGGAGGCGCGCTGGCTGCTCAAGAGCCTGGAGGCGCGTGGCGAAACCCTGCTGCGGGTGGTGCG
CTGCCTGCTGGAACATCAGGCCGGGTTCCTGGAGTTCGGTGCGCAGGCCTTGCGCCCGTTGACGCTGCGCGAGATCGCAG
GCGAGCTCGGCCTGCACGAGTCCACCATTTCCCGCGCAATCGCCCGCAAGTACGTGCGCACCCCGCGCGGCACCATTGCG
CTGCGCGCGTTCTTCGCCTCCGGCATCGACACCGACAGCGGCGGCGAGGCCTCCAGCACTGCCATCCAGGCCATGATCCG
GCGCCTGATCGATGCGGAAAACCCGCGCAAGCCGCTTTCCGACGCCAAGCTGGCCGATCTGCTGAAAACATCCGGCGTCC
CAGTGGCGCGGCGCACCGTTGCGAAGTATCGTGAGGCCATGAACATTTCCGCCTCCCACGAACGCGTGCGCATCGCCTGA

Upstream 100 bases:

>100_bases
ACGTTGCTAGCCAATCCCGACGTCCGTCGCGTGTATCTGGGGGAAACTTTCCGCCTTTGAGCGCGTCGCCGCGCCAACGT
TTTTTCCAGTCAGTGCCCGC

Downstream 100 bases:

>100_bases
CGTTGCAACCACCGCAACGCCGGCCTGCGCCGGGTTTGTCGGATCCCCGGAACGGTTCATTGGCCCACCGACCAATGGAG
GTATCCGATGCGTATCGAGA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKARLQTSLGQQLVMTPQLRQAIKLLQMSTTELEVEIAEAVETNPLLEWADEAAHAASDIAVESAPSASTEEPQREEVAP
AERDDDWSQDELQWTGSGSGGSFDDDESGDAAERVAESETLADHLLWQLHLSPLSPRDRQIGAMLIDALDEDGYLREPLS
AILETLALGAAVDEADVLTVLHQIQRFDPVGVGARTLGECLALQLGVLDADTPGRELALQIVAGPLERLPRAGVAGLAHE
LKRSTADVEQAVQLVRSLDPRPGKQIGDLSQDTYVVPDCVIWRQRGVWRAALAGRAQPKVTIHRGYENLIRSCGESDAGY
LRGQLQEARWLLKSLEARGETLLRVVRCLLEHQAGFLEFGAQALRPLTLREIAGELGLHESTISRAIARKYVRTPRGTIA
LRAFFASGIDTDSGGEASSTAIQAMIRRLIDAENPRKPLSDAKLADLLKTSGVPVARRTVAKYREAMNISASHERVRIA

Sequences:

>Translated_479_residues
MKARLQTSLGQQLVMTPQLRQAIKLLQMSTTELEVEIAEAVETNPLLEWADEAAHAASDIAVESAPSASTEEPQREEVAP
AERDDDWSQDELQWTGSGSGGSFDDDESGDAAERVAESETLADHLLWQLHLSPLSPRDRQIGAMLIDALDEDGYLREPLS
AILETLALGAAVDEADVLTVLHQIQRFDPVGVGARTLGECLALQLGVLDADTPGRELALQIVAGPLERLPRAGVAGLAHE
LKRSTADVEQAVQLVRSLDPRPGKQIGDLSQDTYVVPDCVIWRQRGVWRAALAGRAQPKVTIHRGYENLIRSCGESDAGY
LRGQLQEARWLLKSLEARGETLLRVVRCLLEHQAGFLEFGAQALRPLTLREIAGELGLHESTISRAIARKYVRTPRGTIA
LRAFFASGIDTDSGGEASSTAIQAMIRRLIDAENPRKPLSDAKLADLLKTSGVPVARRTVAKYREAMNISASHERVRIA
>Mature_479_residues
MKARLQTSLGQQLVMTPQLRQAIKLLQMSTTELEVEIAEAVETNPLLEWADEAAHAASDIAVESAPSASTEEPQREEVAP
AERDDDWSQDELQWTGSGSGGSFDDDESGDAAERVAESETLADHLLWQLHLSPLSPRDRQIGAMLIDALDEDGYLREPLS
AILETLALGAAVDEADVLTVLHQIQRFDPVGVGARTLGECLALQLGVLDADTPGRELALQIVAGPLERLPRAGVAGLAHE
LKRSTADVEQAVQLVRSLDPRPGKQIGDLSQDTYVVPDCVIWRQRGVWRAALAGRAQPKVTIHRGYENLIRSCGESDAGY
LRGQLQEARWLLKSLEARGETLLRVVRCLLEHQAGFLEFGAQALRPLTLREIAGELGLHESTISRAIARKYVRTPRGTIA
LRAFFASGIDTDSGGEASSTAIQAMIRRLIDAENPRKPLSDAKLADLLKTSGVPVARRTVAKYREAMNISASHERVRIA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen utilization genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=483, Percent_Identity=42.4430641821946, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 52187; Mature: 52187

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKARLQTSLGQQLVMTPQLRQAIKLLQMSTTELEVEIAEAVETNPLLEWADEAAHAASDI
CCCCHHHHHCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AVESAPSASTEEPQREEVAPAERDDDWSQDELQWTGSGSGGSFDDDESGDAAERVAESET
HHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
LADHLLWQLHLSPLSPRDRQIGAMLIDALDEDGYLREPLSAILETLALGAAVDEADVLTV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
LHQIQRFDPVGVGARTLGECLALQLGVLDADTPGRELALQIVAGPLERLPRAGVAGLAHE
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
LKRSTADVEQAVQLVRSLDPRPGKQIGDLSQDTYVVPDCVIWRQRGVWRAALAGRAQPKV
HHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEECCHHHHHHHCCHHHHHHCCCCCCCE
TIHRGYENLIRSCGESDAGYLRGQLQEARWLLKSLEARGETLLRVVRCLLEHQAGFLEFG
EEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AQALRPLTLREIAGELGLHESTISRAIARKYVRTPRGTIALRAFFASGIDTDSGGEASST
HHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHH
AIQAMIRRLIDAENPRKPLSDAKLADLLKTSGVPVARRTVAKYREAMNISASHERVRIA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEECC
>Mature Secondary Structure
MKARLQTSLGQQLVMTPQLRQAIKLLQMSTTELEVEIAEAVETNPLLEWADEAAHAASDI
CCCCHHHHHCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AVESAPSASTEEPQREEVAPAERDDDWSQDELQWTGSGSGGSFDDDESGDAAERVAESET
HHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
LADHLLWQLHLSPLSPRDRQIGAMLIDALDEDGYLREPLSAILETLALGAAVDEADVLTV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
LHQIQRFDPVGVGARTLGECLALQLGVLDADTPGRELALQIVAGPLERLPRAGVAGLAHE
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
LKRSTADVEQAVQLVRSLDPRPGKQIGDLSQDTYVVPDCVIWRQRGVWRAALAGRAQPKV
HHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEECCHHHHHHHCCHHHHHHCCCCCCCE
TIHRGYENLIRSCGESDAGYLRGQLQEARWLLKSLEARGETLLRVVRCLLEHQAGFLEFG
EEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AQALRPLTLREIAGELGLHESTISRAIARKYVRTPRGTIALRAFFASGIDTDSGGEASST
HHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHH
AIQAMIRRLIDAENPRKPLSDAKLADLLKTSGVPVARRTVAKYREAMNISASHERVRIA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969534 [H]