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The map label for this gene is murD

Identifier: 21243635

GI number: 21243635

Start: 3414570

End: 3415976

Strand: Reverse

Name: murD

Synonym: XAC2908

Alternate gene names: 21243635

Gene position: 3415976-3414570 (Counterclockwise)

Preceding gene: 21243636

Following gene: 21243634

Centisome position: 66.0

GC content: 67.52

Gene sequence:

>1407_bases
GTGCGAATTTCGCAGCTTGAAGGCAAGGCTGTTGCGTTGTGGGGCTGGGGGCGCGAAGGACGTGCGGCGTATCGTGCGCT
GCGGGCGCGCCTGCCGACTCAAGCGCTGACGGTGTTCTGCAACGCCGAAGAGGCGCGTGAGATCGGTGCGCTGGCAGATG
CGGCCTTGCAGGTGGAAACCGATGCCAGCGCGCAGGCGCTGGGACGATTCGAGATCGTCGTGAAGTCGCCCGGCATCAGC
CCGTATCGCGCCGAGGCACTGGCTGCCGCTGCGCAGGGCACGCATTTCATTGGCGGCACCGCGCTGTGGTTTGCCGAACA
CGCGCAGGCGGACGGCAGCGTGCCTGGCGTCGTCTGCATCACCGGCACCAAGGGCAAGAGCACCACCACTGCGCTGCTGG
CGCACGTGCTGCGCGCAGCCGGGCATCGCACCGCCTTGGTCGGAAATATCGGCCAGCCCCTGCTGGAAGTGCTGGCACCG
CAGCCGCCGCCGGCGTATTGGGCGGTCGAGTTGTCCAGCTATCAGACCGGTGATGTCGGGCGCAGCGGCGCGCGTCCCCA
GCTGGCACTGGTGTTGAATCTGTTTCCCGAGCATCTGGACTGGCATGGCGACGAGGCGCGCTATGTGCGCGACAAGCTGT
CGCTGGTGACCGAAGGCCGGCCGCGGATCGCCTTGCTCAACGCTGCCGACCCGCTGCTTGCCGGCTTGCAATTGCCCGAT
AGCCAGGTGCGTTGGTTCAATCAGCCCGCCGGCTGGCATCTGCGTGGCGAGGTGGTCTATCGCGGCGAACAGGCCATTTT
CGATACTGCCGATGTGCCGCTGCCGGGCGAGCACAACCGCCGCAACCTGTGCGCGGTGCTGGCGGCGGTAGAGGCCTTGG
GCCTGGATGCAGCGGCACTTGCGCCGGCGGCAGCGAGCTTTCGTCCATTGCCCAACCGCCTGCAGTTGCTCGGCAGCGTG
GACGGCATCAGCTACGTCAACGATTCGATCAGCACCACGCCGCATGCCTCGCTGGCTGCACTGGCGTGCTTTGCGCGGCG
ACGCGTGGCCTTGCTGGTCGGTGGCCATGATCGCGGGCTGGATTGGCACGATTTCGCCCAGCAGATGGCGCAGCAGGCGC
CGCTGGAAATCGTGACCATGGGCGCCAATGGCCCACGCATCCATGCATTGCTGGCGCCGCTGGCCGAAGCGGGACACTTC
GGCCTGCATGCCGCCAACGATCTCGAACACGCCATGGGACTGGCGCGCAATGCGCTGGGCGAGCAGGGCGGTGTGGTGCT
GCTCTCGCCGGGGGCGCCCAGCTTCGGCGCCTACAGCGATTACGTTGCGCGCGGCCGCCACTTCGCACAGCTGGCAGGCT
TCGACCCGGCGGCGATCAGCGCGATTGACGGACTGGGTGTGCACTGA

Upstream 100 bases:

>100_bases
GACATCCAGCCGCACCTCGACTCGAATGATCTGCAGGTCCAGCCACTCATGGCCATCGATGGCGAGCACCGGATTCCGCC
GGCGCTGTGGGAGCGCGTGC

Downstream 100 bases:

>100_bases
AGGCGGCTAACGCACCGGCTGCGCTCATCAGCCGGTGGGCATGGCAGGTGCTCGGAACTGGCATGTGCCGCTCATACACC
GCTGTGCCTGCGCGCTGTTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRISQLEGKAVALWGWGREGRAAYRALRARLPTQALTVFCNAEEAREIGALADAALQVETDASAQALGRFEIVVKSPGIS
PYRAEALAAAAQGTHFIGGTALWFAEHAQADGSVPGVVCITGTKGKSTTTALLAHVLRAAGHRTALVGNIGQPLLEVLAP
QPPPAYWAVELSSYQTGDVGRSGARPQLALVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIALLNAADPLLAGLQLPD
SQVRWFNQPAGWHLRGEVVYRGEQAIFDTADVPLPGEHNRRNLCAVLAAVEALGLDAAALAPAAASFRPLPNRLQLLGSV
DGISYVNDSISTTPHASLAALACFARRRVALLVGGHDRGLDWHDFAQQMAQQAPLEIVTMGANGPRIHALLAPLAEAGHF
GLHAANDLEHAMGLARNALGEQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIDGLGVH

Sequences:

>Translated_468_residues
MRISQLEGKAVALWGWGREGRAAYRALRARLPTQALTVFCNAEEAREIGALADAALQVETDASAQALGRFEIVVKSPGIS
PYRAEALAAAAQGTHFIGGTALWFAEHAQADGSVPGVVCITGTKGKSTTTALLAHVLRAAGHRTALVGNIGQPLLEVLAP
QPPPAYWAVELSSYQTGDVGRSGARPQLALVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIALLNAADPLLAGLQLPD
SQVRWFNQPAGWHLRGEVVYRGEQAIFDTADVPLPGEHNRRNLCAVLAAVEALGLDAAALAPAAASFRPLPNRLQLLGSV
DGISYVNDSISTTPHASLAALACFARRRVALLVGGHDRGLDWHDFAQQMAQQAPLEIVTMGANGPRIHALLAPLAEAGHF
GLHAANDLEHAMGLARNALGEQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIDGLGVH
>Mature_468_residues
MRISQLEGKAVALWGWGREGRAAYRALRARLPTQALTVFCNAEEAREIGALADAALQVETDASAQALGRFEIVVKSPGIS
PYRAEALAAAAQGTHFIGGTALWFAEHAQADGSVPGVVCITGTKGKSTTTALLAHVLRAAGHRTALVGNIGQPLLEVLAP
QPPPAYWAVELSSYQTGDVGRSGARPQLALVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIALLNAADPLLAGLQLPD
SQVRWFNQPAGWHLRGEVVYRGEQAIFDTADVPLPGEHNRRNLCAVLAAVEALGLDAAALAPAAASFRPLPNRLQLLGSV
DGISYVNDSISTTPHASLAALACFARRRVALLVGGHDRGLDWHDFAQQMAQQAPLEIVTMGANGPRIHALLAPLAEAGHF
GLHAANDLEHAMGLARNALGEQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIDGLGVH

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=466, Percent_Identity=27.8969957081545, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_XANAC (Q8PII9)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643217.1
- ProteinModelPortal:   Q8PII9
- SMR:   Q8PII9
- GeneID:   1156979
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2908
- NMPDR:   fig|190486.1.peg.2861
- HOGENOM:   HBG750024
- OMA:   LWVDDSK
- ProtClustDB:   PRK04690
- BioCyc:   XAXO190486:XAC2908-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49389; Mature: 49389

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISQLEGKAVALWGWGREGRAAYRALRARLPTQALTVFCNAEEAREIGALADAALQVET
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHEEEEECCHHHHHHHHHHHHEEEEEC
DASAQALGRFEIVVKSPGISPYRAEALAAAAQGTHFIGGTALWFAEHAQADGSVPGVVCI
CCCHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCEEECCEEEEHHHHHCCCCCCCEEEEE
TGTKGKSTTTALLAHVLRAAGHRTALVGNIGQPLLEVLAPQPPPAYWAVELSSYQTGDVG
ECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RSGARPQLALVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIALLNAADPLLAGLQLPD
CCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCC
SQVRWFNQPAGWHLRGEVVYRGEQAIFDTADVPLPGEHNRRNLCAVLAAVEALGLDAAAL
HHHHEECCCCCCEEEEEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
APAAASFRPLPNRLQLLGSVDGISYVNDSISTTPHASLAALACFARRRVALLVGGHDRGL
CCCHHCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
DWHDFAQQMAQQAPLEIVTMGANGPRIHALLAPLAEAGHFGLHAANDLEHAMGLARNALG
CHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
EQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIDGLGVH
CCCCEEEECCCCCCCCCHHHHHHCCCHHHHHCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MRISQLEGKAVALWGWGREGRAAYRALRARLPTQALTVFCNAEEAREIGALADAALQVET
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHEEEEECCHHHHHHHHHHHHEEEEEC
DASAQALGRFEIVVKSPGISPYRAEALAAAAQGTHFIGGTALWFAEHAQADGSVPGVVCI
CCCHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCEEECCEEEEHHHHHCCCCCCCEEEEE
TGTKGKSTTTALLAHVLRAAGHRTALVGNIGQPLLEVLAPQPPPAYWAVELSSYQTGDVG
ECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RSGARPQLALVLNLFPEHLDWHGDEARYVRDKLSLVTEGRPRIALLNAADPLLAGLQLPD
CCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCC
SQVRWFNQPAGWHLRGEVVYRGEQAIFDTADVPLPGEHNRRNLCAVLAAVEALGLDAAAL
HHHHEECCCCCCEEEEEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
APAAASFRPLPNRLQLLGSVDGISYVNDSISTTPHASLAALACFARRRVALLVGGHDRGL
CCCHHCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
DWHDFAQQMAQQAPLEIVTMGANGPRIHALLAPLAEAGHFGLHAANDLEHAMGLARNALG
CHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
EQGGVVLLSPGAPSFGAYSDYVARGRHFAQLAGFDPAAISAIDGLGVH
CCCCEEEECCCCCCCCCHHHHHHCCCHHHHHCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217