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The map label for this gene is mexA [H]

Identifier: 21243571

GI number: 21243571

Start: 3336022

End: 3337236

Strand: Reverse

Name: mexA [H]

Synonym: XAC2844

Alternate gene names: 21243571

Gene position: 3337236-3336022 (Counterclockwise)

Preceding gene: 21243573

Following gene: 21243570

Centisome position: 64.48

GC content: 68.89

Gene sequence:

>1215_bases
GTGCGTCCTCATGCCTCGTTTCGCCTGTCGTTGCTTGCTGTTGCCGTTGCCGCCACCGCCTTGCTCAGCGCCTGCGGCAG
CCCGCCGGGAGGCCCGCCGCCACAGGAAGGCACCCCGGAAATGGGCGTGCTGACCGTGAAGCAGCAACCGGTGACGCTCA
CTACCGAGTTGCCGGGGCGCACCGTCCCGTACCTGATCTCCGAAATTCGCCCGCAAGTGGGAGGCATCGTCCAGTCGCGC
CAGTTCACCGAAGGCGGCGACGTCAAGGCTGGCCAGACCCTGTACCAGATCGATCCGGCCCAGTACCGCGCCAGCTATGC
CAGCGCGCAGGCATCCCTTGCCAAGGCCGAGGCGACCCTGCGCACCGCGCAGCTCAAGGCCGAGCGCTACAAGGAGCTGG
CGCAGATCAAAGCGATCAGCCAGCAGGAAGGCGACGACACCGATGCCGCGCTAGGCCAGGCCAAGGCCGACGTGGCAGCC
GGCAAGGCGAGCGTGGAGACCGCGCGCATCAACCTGGCGTTTGCTCGGCTTGACGCACCGATCTCCGGACGCATCGGCCG
CTCCAGCGTGACCCCGGGTGCGCTGGTGACCGCCAACCAGGCCACCGCGCTGACCACCATCCAGCAGCTGGACCCGATCT
ACATCGACGTCACCCAGCCCAGCAGTGCGGTGTTGCGCCTCCGGCAGGCGATGGCACGCGGCGATCTGGAACAAGCCGGC
GACGGTGCGGCCAAGGTGTCGCTGCTGCTGGAAGATGGGAGCACCTACCCGCTGCAGGGGCACCTGGCGTTCTCCGACGT
GACAGTCGACCAGAACACCGGCTCGATCACCCTGCGCGCGGTCTTCCCCAATCCGAACGCCGAGCTGTTGCCGGGCATGT
ACGTGCGCGCGGTGCTGCAGGAAGGCGTCAAGGAGCAGGGCGTGCTGGTGCCGCAGCAGGCGGTGTCGCGCAACGGCGCC
GGCAAACCCACCGCCTTTGTCGTCGGCGCCGACCACAAGCTGCAGTTGCGTGCGCTGGAAACCGACCGTGAGGTGGGCGA
CCAGTGGCTGGTGCGCAGCGGCCTGAAGCCCGGCGAGCAAGTGGTCGTCGAAGGCGCGTCGCGCGCACGCGATGGCATCG
TGGTCAAGACCGTGCCGTGGCAGCCCAAGGCCACGCCCGCCGCCGGGAATGCTGCCGGTCCTTCTGCCCCGACCGCACCG
CGTGCGGCGAACTAG

Upstream 100 bases:

>100_bases
CCGACTGGGTAGAAATGCTTGACGGCTCTGGATGCGCTTATTAGGATACCAAACCGTACCGTTTAGATTTAATCATCACA
CCTCCCGTTGGAGACTGCCC

Downstream 100 bases:

>100_bases
AGGCGGGATCACGTCATGGCACGTTTCTTTATCGATCGTCCGATCTTCGCCTGGGTGTTGGCCATCATCGTGATGCTGGC
CGGCATCCTCTCCATCGCCA

Product: multidrug resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MRPHASFRLSLLAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELPGRTVPYLISEIRPQVGGIVQSR
QFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEATLRTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADVAA
GKASVETARINLAFARLDAPISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQAG
DGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAVLQEGVKEQGVLVPQQAVSRNGA
GKPTAFVVGADHKLQLRALETDREVGDQWLVRSGLKPGEQVVVEGASRARDGIVVKTVPWQPKATPAAGNAAGPSAPTAP
RAAN

Sequences:

>Translated_404_residues
MRPHASFRLSLLAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELPGRTVPYLISEIRPQVGGIVQSR
QFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEATLRTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADVAA
GKASVETARINLAFARLDAPISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQAG
DGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAVLQEGVKEQGVLVPQQAVSRNGA
GKPTAFVVGADHKLQLRALETDREVGDQWLVRSGLKPGEQVVVEGASRARDGIVVKTVPWQPKATPAAGNAAGPSAPTAP
RAAN
>Mature_404_residues
MRPHASFRLSLLAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELPGRTVPYLISEIRPQVGGIVQSR
QFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEATLRTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADVAA
GKASVETARINLAFARLDAPISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQAG
DGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAVLQEGVKEQGVLVPQQAVSRNGA
GKPTAFVVGADHKLQLRALETDREVGDQWLVRSGLKPGEQVVVEGASRARDGIVVKTVPWQPKATPAAGNAAGPSAPTAP
RAAN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=385, Percent_Identity=50.9090909090909, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1786668, Length=376, Percent_Identity=51.8617021276596, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1789929, Length=371, Percent_Identity=46.900269541779, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI87082036, Length=320, Percent_Identity=30, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081796, Length=172, Percent_Identity=29.6511627906977, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42180; Mature: 42180

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPHASFRLSLLAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELPGR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCC
TVPYLISEIRPQVGGIVQSRQFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEATL
HHHHHHHHHCHHHCCHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
RTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADVAAGKASVETARINLAFARLDAP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCC
ISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQAG
CCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCHHHCC
DGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAVLQ
CCCEEEEEEEECCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHH
EGVKEQGVLVPQQAVSRNGAGKPTAFVVGADHKLQLRALETDREVGDQWLVRSGLKPGEQ
HCHHHCCCCCCHHHHHCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCHH
VVVEGASRARDGIVVKTVPWQPKATPAAGNAAGPSAPTAPRAAN
EEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPHASFRLSLLAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELPGR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCC
TVPYLISEIRPQVGGIVQSRQFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEATL
HHHHHHHHHCHHHCCHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
RTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADVAAGKASVETARINLAFARLDAP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCC
ISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQAG
CCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCHHHCC
DGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAVLQ
CCCEEEEEEEECCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHH
EGVKEQGVLVPQQAVSRNGAGKPTAFVVGADHKLQLRALETDREVGDQWLVRSGLKPGEQ
HCHHHCCCCCCHHHHHCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCHH
VVVEGASRARDGIVVKTVPWQPKATPAAGNAAGPSAPTAPRAAN
EEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]