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The map label for this gene is dadA [H]

Identifier: 21243478

GI number: 21243478

Start: 3222971

End: 3224221

Strand: Reverse

Name: dadA [H]

Synonym: XAC2751

Alternate gene names: 21243478

Gene position: 3224221-3222971 (Counterclockwise)

Preceding gene: 21243479

Following gene: 21243477

Centisome position: 62.3

GC content: 67.39

Gene sequence:

>1251_bases
ATGCACGGCAGCCGCGATGATGTGCTGGTGATCGGCGCGGGCGCGATTGGCCTGGCCACCGCGCTGGCACTTGTCGAAGC
GGGCCGGCAGGTGCGTGTGCTCGATGCCGGTGCGACTGGGGCGGCGACTTCCTACGGCAATTGCGGCACCATCACGCCGA
GCCATGCACCGCCGCTGGCCGCGCCCGGCATGATCGCGCAGGCACTGAAATGGATGTTCACCCCGGATGCCCCGCTGTAT
ATCCCGCCCCGTCTGGATCCGGAGTTGTGGCGCTGGCTACTGCGCTTCGCACGATGCTGCAATGCACGCGATTGGCAGTC
CAGTGCACAGGCGCGGGCGGCGCTGTTGCGCGATGCGCGGCAGCGTTTCGACGCGTGGGTGGCGCGGTACGCGCTGGAGT
GCGAGTTTCGCAACGACGGGCTGGATTACGTGTTCGGCGATGCGCGCCGCTTTGCGCAATACCGCGACGAATGCGCGCTG
CTGGCGCAGTGGGGTATCCGGGCAGAGGTCATCGATGGTGCGGACTATATGCGTCAGGATCCCGCATTTCGCGATGGGAT
CGCTGGCGCGATCCACTTCCCGGGCGATGCGCATCTACGCCCGGAGCAATACACCGCAGAGCTTGCGAGAGTGCTGCGCG
CACGTGGCGGGGTGATCGATGCGCACTGCCGCGTGCAGGCAATCGTGCCCGATGGAGGCGGCAGCTGCGTGCAGACCGAG
CACGGCAAGCGCCGTGCGCGCGAGGTAGTGATCGCAACCGGACCATGGTCGCCGGCGCTGGCGGCGCAACTTGGTCTGCG
CCTGCCGGTGCAGGCCGGCAAGGGCTATTCGATTACCTACAGCGCGCCGCATCTGATGCCGCGTCGCCCGGTGGTGTTGA
AGGACCGCTGGGTGTTCGTGGTGCCGTGGCGCGACCGCCTGCGCATCGGCAGCACGATGGAATTTTCCGGGCGCGATACG
CAGCTCAATGCCACGCGCCTGGCTGCGTTGGAGCGCGCGGCGGCCGAATATCTGCATACGCCACGCGGACCTGCAGTGAT
CGAACGCTGGTACGGATGGCGGCCGATGACCTGGGACGACGTGCCGGTCCTGGGCGCGGTGCCAGGCCATCCTCACGTCT
GGCTCGCAGCCGGGCATGGCATGCTCGGCATCAGCATGAGCACTGCAAGCGGACAATTGATGGCCGACCTGATCACGGGC
CGCGCACCTGCGCTGGACCCGCATCCTTACCGGGCGGAGCGTTTCGCATGA

Upstream 100 bases:

>100_bases
TGGTTCCGCTTCGGGCCGATGGAATGGCTATGGCGCAGCGTGACCTATCTGCAGGTGCCGCCGATGTGGCGTGGCGCCGG
GCGCGCCGGGTAGCGCCTGC

Downstream 100 bases:

>100_bases
GTGCGCGTTACGACTACGATGTGGTGATTCTGGGCGGCGGGTCCGGCGGCCTCGCGGCGGGATTTCGCGCGGCAAAACAT
GGCGCGCGCGTGGCGATCAT

Product: D-amino acid dehydrogenase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MHGSRDDVLVIGAGAIGLATALALVEAGRQVRVLDAGATGAATSYGNCGTITPSHAPPLAAPGMIAQALKWMFTPDAPLY
IPPRLDPELWRWLLRFARCCNARDWQSSAQARAALLRDARQRFDAWVARYALECEFRNDGLDYVFGDARRFAQYRDECAL
LAQWGIRAEVIDGADYMRQDPAFRDGIAGAIHFPGDAHLRPEQYTAELARVLRARGGVIDAHCRVQAIVPDGGGSCVQTE
HGKRRAREVVIATGPWSPALAAQLGLRLPVQAGKGYSITYSAPHLMPRRPVVLKDRWVFVVPWRDRLRIGSTMEFSGRDT
QLNATRLAALERAAAEYLHTPRGPAVIERWYGWRPMTWDDVPVLGAVPGHPHVWLAAGHGMLGISMSTASGQLMADLITG
RAPALDPHPYRAERFA

Sequences:

>Translated_416_residues
MHGSRDDVLVIGAGAIGLATALALVEAGRQVRVLDAGATGAATSYGNCGTITPSHAPPLAAPGMIAQALKWMFTPDAPLY
IPPRLDPELWRWLLRFARCCNARDWQSSAQARAALLRDARQRFDAWVARYALECEFRNDGLDYVFGDARRFAQYRDECAL
LAQWGIRAEVIDGADYMRQDPAFRDGIAGAIHFPGDAHLRPEQYTAELARVLRARGGVIDAHCRVQAIVPDGGGSCVQTE
HGKRRAREVVIATGPWSPALAAQLGLRLPVQAGKGYSITYSAPHLMPRRPVVLKDRWVFVVPWRDRLRIGSTMEFSGRDT
QLNATRLAALERAAAEYLHTPRGPAVIERWYGWRPMTWDDVPVLGAVPGHPHVWLAAGHGMLGISMSTASGQLMADLITG
RAPALDPHPYRAERFA
>Mature_416_residues
MHGSRDDVLVIGAGAIGLATALALVEAGRQVRVLDAGATGAATSYGNCGTITPSHAPPLAAPGMIAQALKWMFTPDAPLY
IPPRLDPELWRWLLRFARCCNARDWQSSAQARAALLRDARQRFDAWVARYALECEFRNDGLDYVFGDARRFAQYRDECAL
LAQWGIRAEVIDGADYMRQDPAFRDGIAGAIHFPGDAHLRPEQYTAELARVLRARGGVIDAHCRVQAIVPDGGGSCVQTE
HGKRRAREVVIATGPWSPALAAQLGLRLPVQAGKGYSITYSAPHLMPRRPVVLKDRWVFVVPWRDRLRIGSTMEFSGRDT
QLNATRLAALERAAAEYLHTPRGPAVIERWYGWRPMTWDDVPVLGAVPGHPHVWLAAGHGMLGISMSTASGQLMADLITG
RAPALDPHPYRAERFA

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=426, Percent_Identity=29.1079812206573, Blast_Score=158, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45661; Mature: 45661

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGSRDDVLVIGAGAIGLATALALVEAGRQVRVLDAGATGAATSYGNCGTITPSHAPPLA
CCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
APGMIAQALKWMFTPDAPLYIPPRLDPELWRWLLRFARCCNARDWQSSAQARAALLRDAR
CCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QRFDAWVARYALECEFRNDGLDYVFGDARRFAQYRDECALLAQWGIRAEVIDGADYMRQD
HHHHHHHHHHHEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCC
PAFRDGIAGAIHFPGDAHLRPEQYTAELARVLRARGGVIDAHCRVQAIVPDGGGSCVQTE
CCHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECEEEEEEEECCCCCCEECH
HGKRRAREVVIATGPWSPALAAQLGLRLPVQAGKGYSITYSAPHLMPRRPVVLKDRWVFV
HHHCCCCEEEEEECCCCHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCEEEECCEEEE
VPWRDRLRIGSTMEFSGRDTQLNATRLAALERAAAEYLHTPRGPAVIERWYGWRPMTWDD
ECCHHHEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
VPVLGAVPGHPHVWLAAGHGMLGISMSTASGQLMADLITGRAPALDPHPYRAERFA
CCEEECCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHGSRDDVLVIGAGAIGLATALALVEAGRQVRVLDAGATGAATSYGNCGTITPSHAPPLA
CCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
APGMIAQALKWMFTPDAPLYIPPRLDPELWRWLLRFARCCNARDWQSSAQARAALLRDAR
CCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QRFDAWVARYALECEFRNDGLDYVFGDARRFAQYRDECALLAQWGIRAEVIDGADYMRQD
HHHHHHHHHHHEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCC
PAFRDGIAGAIHFPGDAHLRPEQYTAELARVLRARGGVIDAHCRVQAIVPDGGGSCVQTE
CCHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECEEEEEEEECCCCCCEECH
HGKRRAREVVIATGPWSPALAAQLGLRLPVQAGKGYSITYSAPHLMPRRPVVLKDRWVFV
HHHCCCCEEEEEECCCCHHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCEEEECCEEEE
VPWRDRLRIGSTMEFSGRDTQLNATRLAALERAAAEYLHTPRGPAVIERWYGWRPMTWDD
ECCHHHEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
VPVLGAVPGHPHVWLAAGHGMLGISMSTASGQLMADLITGRAPALDPHPYRAERFA
CCEEECCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA