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The map label for this gene is nuoB [H]

Identifier: 21243431

GI number: 21243431

Start: 3164490

End: 3165065

Strand: Reverse

Name: nuoB [H]

Synonym: XAC2703

Alternate gene names: 21243431

Gene position: 3165065-3164490 (Counterclockwise)

Preceding gene: 21243432

Following gene: 21243430

Centisome position: 61.15

GC content: 64.58

Gene sequence:

>576_bases
GTGGAAGAAGGGAGCGCTGGAATGGGAGTGATTCAGACCCTGGATAGTCTGATGACCAACCCGATGCCGGAAGGCCGGGT
GGAAGACATCCTGCGCCCGGAAGGCGAAAACCCGTTGCTCGAGAAGGGTTACGTGACCACCAGCGTCGATGCGCTGCTGA
ACTGGGCACGCACCGGCTCGATGTGGCCGATGACCTTCGGCCTGGCGTGCTGCGCCGTCGAGATGATGCATGCGGGCGCC
TCGCGCCTGGATCTGGACCGCTACGGCGTGGTGTTCCGCCCGTCGCCGCGCCAGTCCGACGTGATGATCGTGGCCGGCAC
CCTGGTCAACAAGATGGCCCCGGCGCTGCGCAAGGTCTATGACCAGATGCCGGATCCGAAGTGGGTCATCTCGATGGGCA
GCTGCGCCAACGGCGGCGGCTACTATCACTATTCGTATTCGGTGGTGCGCGGTTGCGACCGCATCGTGCCGGTGGACATT
TACGTCCCGGGCTGCCCGCCGACCGCCGAGGCGCTGGTCTACGGCATCTTGCAGCTGCAGAAGAAGATCTGGCGTACCCA
GACGATCGCGCGCTGA

Upstream 100 bases:

>100_bases
TCATCGTGCCGTGGACGCAGGTGTTCATGGAGATCGGCGCACGTTCGCTGGTCACCATGGGGCTGTTCGTCGGTATGTTG
TTCCTCGGCTTTATCTACGT

Downstream 100 bases:

>100_bases
CACCCGTCTTCTTCTACCGAGAGCAGCCAAGCCCCCATGGCAGAGCAAGCATCCTCCTTCACTGACCGACTTGCGGCACG
TTTCGCCGGTGCGCAGATTG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MEEGSAGMGVIQTLDSLMTNPMPEGRVEDILRPEGENPLLEKGYVTTSVDALLNWARTGSMWPMTFGLACCAVEMMHAGA
SRLDLDRYGVVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDI
YVPGCPPTAEALVYGILQLQKKIWRTQTIAR

Sequences:

>Translated_191_residues
MEEGSAGMGVIQTLDSLMTNPMPEGRVEDILRPEGENPLLEKGYVTTSVDALLNWARTGSMWPMTFGLACCAVEMMHAGA
SRLDLDRYGVVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDI
YVPGCPPTAEALVYGILQLQKKIWRTQTIAR
>Mature_191_residues
MEEGSAGMGVIQTLDSLMTNPMPEGRVEDILRPEGENPLLEKGYVTTSVDALLNWARTGSMWPMTFGLACCAVEMMHAGA
SRLDLDRYGVVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDI
YVPGCPPTAEALVYGILQLQKKIWRTQTIAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=158, Percent_Identity=75.3164556962025, Blast_Score=258, Evalue=3e-69,
Organism=Escherichia coli, GI1788624, Length=170, Percent_Identity=46.4705882352941, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=41.8032786885246, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=40.1639344262295, Blast_Score=94, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=151, Percent_Identity=72.1854304635762, Blast_Score=246, Evalue=5e-66,
Organism=Drosophila melanogaster, GI18859983, Length=173, Percent_Identity=70.5202312138728, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24642371, Length=173, Percent_Identity=70.5202312138728, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24651058, Length=189, Percent_Identity=66.6666666666667, Blast_Score=256, Evalue=6e-69,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21037; Mature: 21037

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEGSAGMGVIQTLDSLMTNPMPEGRVEDILRPEGENPLLEKGYVTTSVDALLNWARTGS
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
MWPMTFGLACCAVEMMHAGASRLDLDRYGVVFRPSPRQSDVMIVAGTLVNKMAPALRKVY
CCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHH
DQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALVYGILQLQ
HHCCCCCEEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
KKIWRTQTIAR
HHHHHHHHCCC
>Mature Secondary Structure
MEEGSAGMGVIQTLDSLMTNPMPEGRVEDILRPEGENPLLEKGYVTTSVDALLNWARTGS
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
MWPMTFGLACCAVEMMHAGASRLDLDRYGVVFRPSPRQSDVMIVAGTLVNKMAPALRKVY
CCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHH
DQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALVYGILQLQ
HHCCCCCEEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
KKIWRTQTIAR
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA