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The map label for this gene is truB

Identifier: 21243414

GI number: 21243414

Start: 3143658

End: 3144584

Strand: Reverse

Name: truB

Synonym: XAC2685

Alternate gene names: 21243414

Gene position: 3144584-3143658 (Counterclockwise)

Preceding gene: 21243415

Following gene: 21243413

Centisome position: 60.76

GC content: 65.91

Gene sequence:

>927_bases
TTGAAACCCCGCATCGTCTACCGCCCGTTGCACGGCATCCTGCTGCTGGACAAGCCGTCGGGGCTGAGCTCCAACAATGC
ACTGCAGGCCGCACGCCGGTTATTGCGTGCAGAGAAGGGCGGCCATACCGGCAGCCTGGATCCGCTGGCCACCGGCCTGT
TGCCGCTCTGTTTTGGCGAAGCGACCAAGATCGCCGGACTGCTGCTCGGTTCAGCCAAGGCGTACGACGCCGAGATCGTG
TTGGGCGTCACCACCGACACCGACGATGCCGACGGCGAGTCGCTGCGCGAGCGTGCGGTGCCGGATCTGAGCGAGGCCGA
TCTCCAGGCGGCGCTGGCGCCTTTCATCGGCCGTATCCAGCAACAGGCGCCGATTTATTCCGCGCTCAAGCAGGGCGGCG
AGCCGCTGTACGCCAAGGCGCGACGCGGCGAACGTATCGAAGCGCCGGTGCGCGAGGTGGATGTGCAGGCGATCGAGGTG
CTGGGCTACGGCGCCCCACGTCTGCGCCTGCGCGTGACCTGCGGTTCGGGCACGTATATCCGCAGCCTGGCGCGTGACCT
GGGCGAGGCGCTGGGATGTGGAGCGCACATCGCCTCGCTGCGTCGGCTGTGGGTGGAGCCGTTTCGCGCTCCGCAGATGA
TCACGCTGGAGGCGTTGTCGGCAGTTTTGGAAGCGGGTGCCGAGGCGCAGACACTATTGCTGCCGATCGAGGCCGGCCTG
GCCGATTTTGCGCGAATCGTGCTCGATCAAACCCGCGCGGCGCGTTTTCGCATGGGCCAGCGCTTGCGTGATGCCGCATT
TCCAACGGGGCAGGTGGCCGTATTCGGACCTGACGGAACCCCCTCCGGACTAGGCCTGGTGGACGCCGACGGGCGCTTGT
CGCCACAACGATTATTCAATGGACTGAACGAAATTCCGGCCTGTTAA

Upstream 100 bases:

>100_bases
GAAGATGCCGAACAGCGCTGAGCGCTGTCACGGCCTGCCGCTTCTGCCGCGGCCCTGATTCCCGCGGGCGCGCACTGCGC
CTGATTCGAGCACCGATCCT

Downstream 100 bases:

>100_bases
GATTTTGTCAACTTGTTCCCTGGGCCGGGCGACGTTACAATTTCGCGGCCCCGATCGGGGCACCTTAGCGCCAAGCGCGC
TCATCCTAAGCAAACGGCGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKPRIVYRPLHGILLLDKPSGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGEATKIAGLLLGSAKAYDAEIV
LGVTTDTDDADGESLRERAVPDLSEADLQAALAPFIGRIQQQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIEV
LGYGAPRLRLRVTCGSGTYIRSLARDLGEALGCGAHIASLRRLWVEPFRAPQMITLEALSAVLEAGAEAQTLLLPIEAGL
ADFARIVLDQTRAARFRMGQRLRDAAFPTGQVAVFGPDGTPSGLGLVDADGRLSPQRLFNGLNEIPAC

Sequences:

>Translated_308_residues
MKPRIVYRPLHGILLLDKPSGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGEATKIAGLLLGSAKAYDAEIV
LGVTTDTDDADGESLRERAVPDLSEADLQAALAPFIGRIQQQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIEV
LGYGAPRLRLRVTCGSGTYIRSLARDLGEALGCGAHIASLRRLWVEPFRAPQMITLEALSAVLEAGAEAQTLLLPIEAGL
ADFARIVLDQTRAARFRMGQRLRDAAFPTGQVAVFGPDGTPSGLGLVDADGRLSPQRLFNGLNEIPAC
>Mature_308_residues
MKPRIVYRPLHGILLLDKPSGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGEATKIAGLLLGSAKAYDAEIV
LGVTTDTDDADGESLRERAVPDLSEADLQAALAPFIGRIQQQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIEV
LGYGAPRLRLRVTCGSGTYIRSLARDLGEALGCGAHIASLRRLWVEPFRAPQMITLEALSAVLEAGAEAQTLLLPIEAGL
ADFARIVLDQTRAARFRMGQRLRDAAFPTGQVAVFGPDGTPSGLGLVDADGRLSPQRLFNGLNEIPAC

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=216, Percent_Identity=35.6481481481481, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI215599015, Length=195, Percent_Identity=31.2820512820513, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI4503337, Length=195, Percent_Identity=31.2820512820513, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI2367200, Length=308, Percent_Identity=42.8571428571429, Blast_Score=223, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17553978, Length=191, Percent_Identity=28.7958115183246, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=173, Percent_Identity=33.5260115606936, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=260, Percent_Identity=28.0769230769231, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364189, Length=260, Percent_Identity=25.3846153846154, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364187, Length=260, Percent_Identity=25.3846153846154, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364185, Length=260, Percent_Identity=25.3846153846154, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364183, Length=260, Percent_Identity=25.3846153846154, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI62471759, Length=260, Percent_Identity=25.3846153846154, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17975520, Length=260, Percent_Identity=25.3846153846154, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_XANAC (Q8PJ57)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642996.1
- ProteinModelPortal:   Q8PJ57
- SMR:   Q8PJ57
- GeneID:   1156756
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2685
- NMPDR:   fig|190486.1.peg.2640
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK04642
- BioCyc:   XAXO190486:XAC2685-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 32789; Mature: 32789

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRIVYRPLHGILLLDKPSGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGE
CCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
ATKIAGLLLGSAKAYDAEIVLGVTTDTDDADGESLRERAVPDLSEADLQAALAPFIGRIQ
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIEVLGYGAPRLRLRVTCGSGTYI
HCCHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHH
RSLARDLGEALGCGAHIASLRRLWVEPFRAPQMITLEALSAVLEAGAEAQTLLLPIEAGL
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHH
ADFARIVLDQTRAARFRMGQRLRDAAFPTGQVAVFGPDGTPSGLGLVDADGRLSPQRLFN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHC
GLNEIPAC
CHHHCCCC
>Mature Secondary Structure
MKPRIVYRPLHGILLLDKPSGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGE
CCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
ATKIAGLLLGSAKAYDAEIVLGVTTDTDDADGESLRERAVPDLSEADLQAALAPFIGRIQ
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QQAPIYSALKQGGEPLYAKARRGERIEAPVREVDVQAIEVLGYGAPRLRLRVTCGSGTYI
HCCHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHH
RSLARDLGEALGCGAHIASLRRLWVEPFRAPQMITLEALSAVLEAGAEAQTLLLPIEAGL
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHH
ADFARIVLDQTRAARFRMGQRLRDAAFPTGQVAVFGPDGTPSGLGLVDADGRLSPQRLFN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHC
GLNEIPAC
CHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217