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The map label for this gene is pnp

Identifier: 21243412

GI number: 21243412

Start: 3140958

End: 3143072

Strand: Reverse

Name: pnp

Synonym: XAC2683

Alternate gene names: 21243412

Gene position: 3143072-3140958 (Counterclockwise)

Preceding gene: 21243413

Following gene: 21243408

Centisome position: 60.73

GC content: 63.07

Gene sequence:

>2115_bases
GTGGCAAAAATCACCAAAACCTTCCAGTACGGCAAGCACACCGTCACTCTTGAGACGGGCGAAATCGCTCGCCAGGCAGG
CGGCGCCGTGATCGTCAAGGTCGACGACACCGTACTGCTGGTCACCGCCGTCGCCGCCAAGAGCGCGCGCGAAGGGCAGG
ACTTCTTTCCTCTGACGGTCGATTATCAGGAGAAGTTCTACGCTGGCGGCCGCATCCCGGGCGGCTTCTTCAAGCGCGAA
GGACGCGCGACCGAGAAGGAAACGTTGATCTCGCGCCTGATCGATCGTCCGATCCGTCCGCTGTTCCCGGAGGACTACAA
GAACGAAGTGCAGATCATCGCCACCGTGATGTCGATGAACCCGGACATCGACGGCGATATCGCCGCGCTGATCGGCGCCT
CGGCTGCACTGTCGCTGGCAGGCACTCCCTTCAAGGGCCCGATCGGCGCCGCCAAGGTCGGCTACAAGAACGGCGAATAC
ATCCTCAACCCGACCGTGACCGAGCTGAAGGATTCGCAGCTGGAGTTGGTCGTGGCCGGTACCGCCAACGCGGTGCTGAT
GGTCGAGTCCGAAGCCGAGTTGCTGTCCGAAGAAGTCATGCTGGGCGCAGTGACGTTCGGCCATCGCGAGATGCAGAAGG
TCATCAACGTCATCAACGAGCTGACCGTCGAGGCCGGCACCAAGCCGTCCGACTGGGTGGCCCCGGCGAAGAACGACGGC
ATGATCGCTGCCCTGAAGGAAGCGGTGGGCGACCAGCTGGCGTCGGCGTTCCAGGTGCGCGACAAGCTGCAGCGCCGCGA
TGCGATTTCCGCGATCAAGAAGGACGTGCTGGGCGCACTGGCGCCGCGCGCCACCATCGAAGGCTGGGCTGCCGGTGATC
TGGCCAAGGAATTCGGCGAGCTGGAATACCAGACCATGCGTGGTTCGGTGTTGAGCACCAAGGTGCGTATCGACGGTCGT
GCACTGGATACCGTGCGTCCGATCAGCGCCACGGCCGGTGTGTTGCCGCGTACCCATGGCTCGGCGCTGTTTACCCGCGG
CGAGACGCAGGCGATCGTGATCACCACGCTGGGCACCGCGCGCGACGGTCAGGTCATCGACGCGGTGTCGGGCGAGTACA
AGGAAAATTTCCTGTTCCATTACAACTTCCCTCCGTACTCGGTGGGCGAGTGCGGTCGTTTCGGTGCGCCGAAGCGTCGC
GAAATCGGCCACGGCCGCCTGGCCAAGCGCGGCGTGCTGGCAGTGATGCCGACCCTGGAAGAATTCCCGTACACCATCCG
CGTGGTCTCGGAAATCACCGAATCCAACGGTTCGTCCTCGATGGCATCGGTCTGCGGCAGCTCGCTGGCGCTGATGGATG
CTGGCGTGCCGATCAAGGCGCCGGTCGCGGGTATCGCAATGGGCCTGGTCAAGGAAGGCAACGACTTCGTCGTGCTCTCC
GACATCCTGGGTGACGAAGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGTACTGCCGAAGGCGTGTCCGCCCTGCA
GATGGACATCAAGATCGAAGGCATCACCGAAGAGATCATGAAGCAGGCATTGCAGCAGGCCAAGGCTGGCCGTCTGCACA
TCCTGGGCGAGATGGCGCATGCGCTGACCACCCCGCGTCAGGAGCTGAGCGACTACGCGCCGCGCCTGCTCACCATCAAG
ATCCACCCGGACAAGATCCGCGAAGTGATCGGCAAGGGTGGCTCGACCATCCAGGCCATCACCAAGGAAACCGGCACGCA
GATCGACATCCAGGACGATGGCACGATCATCATCGCCTCGGTCAATGCCATCGCTGCGCAGGCTGCGAAGTCGCGCATCG
AACAGATCACCTCGGACGTGGAGCCGGGCCGCATCTACGAAGGCAAGGTCGCCAAGATCATGGACTTCGGTGCGTTCGTC
ACCATCCTGCCGGGCAAGGACGGCCTGGTGCACGTCTCGCAGATTTCCAGCGAGCGCGTGGAGAAGGTCGGCGACAAGTT
GAAGGAAGGCGATCTGGTCCGCGTCAAGGTGCTGGAAGTGGACAAGCAGGGCCGCATCCGCCTGTCGATCAAGGCAGTGG
AAGAAGGCGAAGGCGTGCAGGCGTCGGCCGAGTAA

Upstream 100 bases:

>100_bases
GCAATACGCACTCTTGGGCCGGAGCCTCCCGGCCGCCCGTTCTCGGCATGGGTCGACGACGGTCCAACGCAGACAGCAAT
ACCTAAGGGAAAAAACCTCC

Downstream 100 bases:

>100_bases
TCGCTGCGCGCTGATGCATCACGCGTGATGCATGACGAAAGAGCGGGCTTCGGCCCGCTTTTTTGTTGTGTGGGATTGGT
ATTAATTCCTTCTCCCATCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MAKITKTFQYGKHTVTLETGEIARQAGGAVIVKVDDTVLLVTAVAAKSAREGQDFFPLTVDYQEKFYAGGRIPGGFFKRE
GRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMNPDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEY
ILNPTVTELKDSQLELVVAGTANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNDG
MIAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGELEYQTMRGSVLSTKVRIDGR
ALDTVRPISATAGVLPRTHGSALFTRGETQAIVITTLGTARDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRR
EIGHGRLAKRGVLAVMPTLEEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLS
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAHALTTPRQELSDYAPRLLTIK
IHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIASVNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFV
TILPGKDGLVHVSQISSERVEKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE

Sequences:

>Translated_704_residues
MAKITKTFQYGKHTVTLETGEIARQAGGAVIVKVDDTVLLVTAVAAKSAREGQDFFPLTVDYQEKFYAGGRIPGGFFKRE
GRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMNPDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEY
ILNPTVTELKDSQLELVVAGTANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNDG
MIAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGELEYQTMRGSVLSTKVRIDGR
ALDTVRPISATAGVLPRTHGSALFTRGETQAIVITTLGTARDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRR
EIGHGRLAKRGVLAVMPTLEEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLS
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAHALTTPRQELSDYAPRLLTIK
IHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIASVNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFV
TILPGKDGLVHVSQISSERVEKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE
>Mature_703_residues
AKITKTFQYGKHTVTLETGEIARQAGGAVIVKVDDTVLLVTAVAAKSAREGQDFFPLTVDYQEKFYAGGRIPGGFFKREG
RATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMNPDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEYI
LNPTVTELKDSQLELVVAGTANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNDGM
IAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGELEYQTMRGSVLSTKVRIDGRA
LDTVRPISATAGVLPRTHGSALFTRGETQAIVITTLGTARDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRRE
IGHGRLAKRGVLAVMPTLEEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLSD
ILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAHALTTPRQELSDYAPRLLTIKI
HPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIASVNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFVT
ILPGKDGLVHVSQISSERVEKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=712, Percent_Identity=36.376404494382, Blast_Score=426, Evalue=1e-119,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=64.3165467625899, Blast_Score=888, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=82, Percent_Identity=46.3414634146341, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115534063, Length=706, Percent_Identity=33.0028328611898, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17535281, Length=81, Percent_Identity=46.9135802469136, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281362905, Length=713, Percent_Identity=37.0266479663394, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=713, Percent_Identity=37.0266479663394, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=713, Percent_Identity=37.0266479663394, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=653, Percent_Identity=36.6003062787136, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_XANAC (Q8PJ59)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642994.1
- HSSP:   P05055
- ProteinModelPortal:   Q8PJ59
- SMR:   Q8PJ59
- GeneID:   1156754
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2683
- NMPDR:   fig|190486.1.peg.2638
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   XAXO190486:XAC2683-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75435; Mature: 75304

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITKTFQYGKHTVTLETGEIARQAGGAVIVKVDDTVLLVTAVAAKSAREGQDFFPLTV
CCCCCCHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEHHHHHHCCCCCCCCEEEE
DYQEKFYAGGRIPGGFFKREGRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMN
CCCHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEYILNPTVTELKDSQLELVVAG
CCCCCCHHHHHHCHHHHEECCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCCCEEEEEEC
TANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNDG
CCCEEEEEECHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MIAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
LEYQTMRGSVLSTKVRIDGRALDTVRPISATAGVLPRTHGSALFTRGETQAIVITTLGTA
CHHHHHCCCEEEEEEEECCEEECCCCCCHHHCCCCCCCCCCEEEECCCCCEEEEEEECCC
RDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRREIGHGRLAKRGVLAVMPTLE
CCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCHH
EFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLS
HCCCCEEEHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEH
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAH
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCEEEHHHHHH
ALTTPRQELSDYAPRLLTIKIHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIAS
HHCCCHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCEEEEEE
VNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFVTILPGKDGLVHVSQISSERV
CHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHEECCCEEEEECCCCCEEEHHHHHHHHH
EKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
AKITKTFQYGKHTVTLETGEIARQAGGAVIVKVDDTVLLVTAVAAKSAREGQDFFPLTV
CCCCCHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEHHHHHHCCCCCCCCEEEE
DYQEKFYAGGRIPGGFFKREGRATEKETLISRLIDRPIRPLFPEDYKNEVQIIATVMSMN
CCCHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDIDGDIAALIGASAALSLAGTPFKGPIGAAKVGYKNGEYILNPTVTELKDSQLELVVAG
CCCCCCHHHHHHCHHHHEECCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCCCEEEEEEC
TANAVLMVESEAELLSEEVMLGAVTFGHREMQKVINVINELTVEAGTKPSDWVAPAKNDG
CCCEEEEEECHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MIAALKEAVGDQLASAFQVRDKLQRRDAISAIKKDVLGALAPRATIEGWAAGDLAKEFGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
LEYQTMRGSVLSTKVRIDGRALDTVRPISATAGVLPRTHGSALFTRGETQAIVITTLGTA
CHHHHHCCCEEEEEEEECCEEECCCCCCHHHCCCCCCCCCCEEEECCCCCEEEEEEECCC
RDGQVIDAVSGEYKENFLFHYNFPPYSVGECGRFGAPKRREIGHGRLAKRGVLAVMPTLE
CCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCHH
EFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPIKAPVAGIAMGLVKEGNDFVVLS
HCCCCEEEHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEH
DILGDEDHLGDMDFKVAGTAEGVSALQMDIKIEGITEEIMKQALQQAKAGRLHILGEMAH
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCEEEHHHHHH
ALTTPRQELSDYAPRLLTIKIHPDKIREVIGKGGSTIQAITKETGTQIDIQDDGTIIIAS
HHCCCHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCEEEEEE
VNAIAAQAAKSRIEQITSDVEPGRIYEGKVAKIMDFGAFVTILPGKDGLVHVSQISSERV
CHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHEECCCEEEEECCCCCEEEHHHHHHHHH
EKVGDKLKEGDLVRVKVLEVDKQGRIRLSIKAVEEGEGVQASAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217