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The map label for this gene is yieO [H]

Identifier: 21243227

GI number: 21243227

Start: 2909542

End: 2910957

Strand: Direct

Name: yieO [H]

Synonym: XAC2494

Alternate gene names: 21243227

Gene position: 2909542-2910957 (Clockwise)

Preceding gene: 21243226

Following gene: 21243230

Centisome position: 56.22

GC content: 66.67

Gene sequence:

>1416_bases
GTGTCCGAGGCCGGCGCTACGCCGCCGCGTTATCCCGATTACCGCATCATCTCGCTGATCCTGGCTTGCGCCATTTTCAT
GGAGCAGATGGATGCCACCGTGCTGGCCACCGCGCTGCCGACGCTGGCGCGCGATTTCGGCGTGGCGGCGCCGGCCATGA
GCATCGCCATGACCAGCTACCTGCTGGCCCTGGCGGTGCTGATCCCGGCCAGCGGCGCCATCGCCGACCGCTTCGGCCTG
CGCCGCGTGTTCGGCGCCTCCATCTGGGTGTTCGTCGGTGGTTCGATCCTGTGTTCGCTGGCCGACAGCCTGCCCACCAT
GGTGGCCGCGCGCGTGCTGCAGGGCGCAGGCGGCGCCATGATGGCCCCGCTGGGCCGGCTGATCCTGCTGCGCACGGTGG
AACGCCGCCATCTGGTGTCGGCGATGGCCTGGACGCTGGTGCCGGCCTTCATCGGCCCGATGCTCGGCCCGCCGCTGGGC
GGCTTTTTCGTCTCGTACCTGGATTGGCGCTGGATCTTCTATATCAACGTGCCGATCGGTATCGCCGGCTTCCTGCTGGT
GCGGCGCTTCATTCCCGAGATTCCCACCGAATCGGGGCCAGCGCGCTTCGACCTGCGCGGCTTCGTGCTGTGCGGCACTG
CGCTCGGTTGCCTGCTGTTCGGCCTGGAAATGGTCAGCCAGCAAAACGGCATCGGCACGGCCAGCTGGTTGCTGGCGATC
GGCGGCAGCGCTGCGCTGGGGTATCTCTGGCACGCGCGTCACCACCCTGCTCCGCTGCTGGATCTGAGCTTGCTGCGGAT
CGACTCGTTCCGGTTGTCGGTGATCGGCGGCGCATTGATGCGCATCACCCAGGGCGCGCATCCGTTTCTGCTGCCGCTGC
TGTTTCAGATCGGCTTCGGCATGAGCGCTGCGCACAGCGGCCGCCTGATCCTGGCCACCGCGCTCGGCGCGTTGTTGATG
CGCAGCATCACCCCGCAATTGCTGCGCCGCTTCGGTTATCGCAACAGCCTGATCGGCAACGGTGTGCTCGCCAGCCTGGG
CTACATGGTGTGCGCGTTGTTCCGCCCCGATTGGCCGCCGGCGCTGATGTTCGGCCTGTTGCTGTGCTGCGGCGCCTTCA
TGTCGTTCCAGTTCGCCGACTACAACACCATCGCCTACGAGAACGTGCCTGCCGCGTGCATGAGCCGCGCCAGCAGCCTG
TATACGACCTTGCAGCAACTGATGCTCTCGGTGGGCGTGTGCGCCGGTGCGATGATCCTCAAACTGGCGATGCTGGCCGG
CGATCACAGCCAGCCTGCGCAACGCGATTTCTCGCTGGCGTTCTGCGTGGTCAGCCTGATCTCGCTCAGCGCCACCTGGT
GGCATCTGCGCTTCGACAAGCATGCCGGGCAGGAAATGAGCGGGCATCGGGCGTGA

Upstream 100 bases:

>100_bases
CATGTTGCGCCGCACAACACCGTGCGACGCGGGCCGACCCTGCAGTCGTCTGCGACGCAGGCGGCGCGGCCCCGCAACCG
AGAGTCCGATGTCCTCCAGC

Downstream 100 bases:

>100_bases
GGTAAGGGGAGATATCGCTGACGCTCCAGCCGAGCCGCTTCGGCTGTGTGACGAACCCAACTCCAACCTCTTCTTGCGTG
CTGCATCAAGCAGACGACTA

Product: drug resistance translocase

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSEAGATPPRYPDYRIISLILACAIFMEQMDATVLATALPTLARDFGVAAPAMSIAMTSYLLALAVLIPASGAIADRFGL
RRVFGASIWVFVGGSILCSLADSLPTMVAARVLQGAGGAMMAPLGRLILLRTVERRHLVSAMAWTLVPAFIGPMLGPPLG
GFFVSYLDWRWIFYINVPIGIAGFLLVRRFIPEIPTESGPARFDLRGFVLCGTALGCLLFGLEMVSQQNGIGTASWLLAI
GGSAALGYLWHARHHPAPLLDLSLLRIDSFRLSVIGGALMRITQGAHPFLLPLLFQIGFGMSAAHSGRLILATALGALLM
RSITPQLLRRFGYRNSLIGNGVLASLGYMVCALFRPDWPPALMFGLLLCCGAFMSFQFADYNTIAYENVPAACMSRASSL
YTTLQQLMLSVGVCAGAMILKLAMLAGDHSQPAQRDFSLAFCVVSLISLSATWWHLRFDKHAGQEMSGHRA

Sequences:

>Translated_471_residues
MSEAGATPPRYPDYRIISLILACAIFMEQMDATVLATALPTLARDFGVAAPAMSIAMTSYLLALAVLIPASGAIADRFGL
RRVFGASIWVFVGGSILCSLADSLPTMVAARVLQGAGGAMMAPLGRLILLRTVERRHLVSAMAWTLVPAFIGPMLGPPLG
GFFVSYLDWRWIFYINVPIGIAGFLLVRRFIPEIPTESGPARFDLRGFVLCGTALGCLLFGLEMVSQQNGIGTASWLLAI
GGSAALGYLWHARHHPAPLLDLSLLRIDSFRLSVIGGALMRITQGAHPFLLPLLFQIGFGMSAAHSGRLILATALGALLM
RSITPQLLRRFGYRNSLIGNGVLASLGYMVCALFRPDWPPALMFGLLLCCGAFMSFQFADYNTIAYENVPAACMSRASSL
YTTLQQLMLSVGVCAGAMILKLAMLAGDHSQPAQRDFSLAFCVVSLISLSATWWHLRFDKHAGQEMSGHRA
>Mature_470_residues
SEAGATPPRYPDYRIISLILACAIFMEQMDATVLATALPTLARDFGVAAPAMSIAMTSYLLALAVLIPASGAIADRFGLR
RVFGASIWVFVGGSILCSLADSLPTMVAARVLQGAGGAMMAPLGRLILLRTVERRHLVSAMAWTLVPAFIGPMLGPPLGG
FFVSYLDWRWIFYINVPIGIAGFLLVRRFIPEIPTESGPARFDLRGFVLCGTALGCLLFGLEMVSQQNGIGTASWLLAIG
GSAALGYLWHARHHPAPLLDLSLLRIDSFRLSVIGGALMRITQGAHPFLLPLLFQIGFGMSAAHSGRLILATALGALLMR
SITPQLLRRFGYRNSLIGNGVLASLGYMVCALFRPDWPPALMFGLLLCCGAFMSFQFADYNTIAYENVPAACMSRASSLY
TTLQQLMLSVGVCAGAMILKLAMLAGDHSQPAQRDFSLAFCVVSLISLSATWWHLRFDKHAGQEMSGHRA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=451, Percent_Identity=36.8070953436807, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788392, Length=408, Percent_Identity=37.0098039215686, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=28.0952380952381, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788710, Length=406, Percent_Identity=28.3251231527094, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1789042, Length=405, Percent_Identity=26.9135802469136, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6325455, Length=163, Percent_Identity=31.2883435582822, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=233, Percent_Identity=26.6094420600858, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50706; Mature: 50575

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAGATPPRYPDYRIISLILACAIFMEQMDATVLATALPTLARDFGVAAPAMSIAMTSY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LLALAVLIPASGAIADRFGLRRVFGASIWVFVGGSILCSLADSLPTMVAARVLQGAGGAM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MAPLGRLILLRTVERRHLVSAMAWTLVPAFIGPMLGPPLGGFFVSYLDWRWIFYINVPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEECCHH
IAGFLLVRRFIPEIPTESGPARFDLRGFVLCGTALGCLLFGLEMVSQQNGIGTASWLLAI
HHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GGSAALGYLWHARHHPAPLLDLSLLRIDSFRLSVIGGALMRITQGAHPFLLPLLFQIGFG
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
MSAAHSGRLILATALGALLMRSITPQLLRRFGYRNSLIGNGVLASLGYMVCALFRPDWPP
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCH
ALMFGLLLCCGAFMSFQFADYNTIAYENVPAACMSRASSLYTTLQQLMLSVGVCAGAMIL
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAMLAGDHSQPAQRDFSLAFCVVSLISLSATWWHLRFDKHAGQEMSGHRA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECHHCCCCCCCCCC
>Mature Secondary Structure 
SEAGATPPRYPDYRIISLILACAIFMEQMDATVLATALPTLARDFGVAAPAMSIAMTSY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LLALAVLIPASGAIADRFGLRRVFGASIWVFVGGSILCSLADSLPTMVAARVLQGAGGAM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MAPLGRLILLRTVERRHLVSAMAWTLVPAFIGPMLGPPLGGFFVSYLDWRWIFYINVPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEECCHH
IAGFLLVRRFIPEIPTESGPARFDLRGFVLCGTALGCLLFGLEMVSQQNGIGTASWLLAI
HHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GGSAALGYLWHARHHPAPLLDLSLLRIDSFRLSVIGGALMRITQGAHPFLLPLLFQIGFG
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
MSAAHSGRLILATALGALLMRSITPQLLRRFGYRNSLIGNGVLASLGYMVCALFRPDWPP
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCH
ALMFGLLLCCGAFMSFQFADYNTIAYENVPAACMSRASSLYTTLQQLMLSVGVCAGAMIL
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLAMLAGDHSQPAQRDFSLAFCVVSLISLSATWWHLRFDKHAGQEMSGHRA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]