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The map label for this gene is rpfG [H]

Identifier: 21243226

GI number: 21243226

Start: 2908126

End: 2909112

Strand: Direct

Name: rpfG [H]

Synonym: XAC2493

Alternate gene names: 21243226

Gene position: 2908126-2909112 (Clockwise)

Preceding gene: 21243225

Following gene: 21243227

Centisome position: 56.19

GC content: 66.57

Gene sequence:

>987_bases
ATGCCGTCCCGCCCCCTGCTGTGTGTCGACGACGAGTCCAGCAACCTGGCCACCTTGCGCCAGCTGTTGCGCGACGACTT
CGCACTGGTCTTCGCCAAGTCCGGCGGCGAAGCCCTGGATGCGGTCAGCCGCCACGCCCCAAAGCTGATCCTGCTGGATG
TCGAATTGCCCGACATGGACGGCTACGCGGTGGCGCGTGCGCTCAAGCAACAGCCGTCCAGCAACGCCATCCCCATCCTG
TTCGTGACCTCGCGCAACAGCGAACACGACGAACGCCTCGGGCTGGAAGCGGGCGCGGCCGATTACGTCAGCAAGCCCTA
CTCGCCGGCGCTGCTCAAGGCACGCATCGGTACCCAGTTGAAGCTGGCCGAAAACGCACGTCTTGCCCAGCAATACCGCG
ACGCGATCCATCTGCTCGGCACCGCCGGCCAGGGCCAGGACGCCGACACCGGCGCGCATATCTGGCGCATGGCGGTGTAT
GCGCGGGTCCTGGCCGAAGCGGTGGGCTGGTCGCCGCAGCAGGCGCAGACCCTGCAGGACGCCGCCCCGCTGCACGACGC
CGGCAAGATCGCAGTGCCCGGCAGCATCCTGCAAAAGCCCGATGCGCTGGACGAACACGAACGCTCGGTGGTGCGCCAGC
ACCCGCAAGCCGGCCATGATCTGTTGCGCCACGGCCGCGGCCCGGTGTTTCGGTTGGCAGCGGAAATCGCACTGCATCAT
CACGAATGCTGGGACGGCAGCGGCTATCCCCAAGGCTTGTCAGGCGATGCGATTCCCGAATCGGCGCGCATCGTGGCGGT
GGCCGACGTGTTCGATGCACTGTGCGGGCGACGCGCCTACAAGGCGCCGTGGACGGTGGAGGACGCCATGCGCAAGCTCG
ACAGCATGGCAGGCAAACAACTGGAACCGCGCCTGGTGCAGCATTTCCACGAGGCATTGCCGCAGATTCTGCAGATCAAG
GACGCTTGGGACAGCCCAGTGGCGTAG

Upstream 100 bases:

>100_bases
CGAAGATTTCGACTTCCGCCGCGCCGAAACCACGTTGCGCGAGTGGCAAACCACCCACCCGCACTAATCGCGCTCCCCGA
GCGTTTGGCAAGGATCGACG

Downstream 100 bases:

>100_bases
CGCTGATCCGGGCAGGTCCCCGCGCTGATGCAGGCAGGCGAACGATCGCGAAACGCGATTTGATGTCGGCGGTCCTTCGC
CGCAGCCTCATCCAAACCCT

Product: two-component system regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVSRHAPKLILLDVELPDMDGYAVARALKQQPSSNAIPIL
FVTSRNSEHDERLGLEAGAADYVSKPYSPALLKARIGTQLKLAENARLAQQYRDAIHLLGTAGQGQDADTGAHIWRMAVY
ARVLAEAVGWSPQQAQTLQDAAPLHDAGKIAVPGSILQKPDALDEHERSVVRQHPQAGHDLLRHGRGPVFRLAAEIALHH
HECWDGSGYPQGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQLEPRLVQHFHEALPQILQIK
DAWDSPVA

Sequences:

>Translated_328_residues
MPSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVSRHAPKLILLDVELPDMDGYAVARALKQQPSSNAIPIL
FVTSRNSEHDERLGLEAGAADYVSKPYSPALLKARIGTQLKLAENARLAQQYRDAIHLLGTAGQGQDADTGAHIWRMAVY
ARVLAEAVGWSPQQAQTLQDAAPLHDAGKIAVPGSILQKPDALDEHERSVVRQHPQAGHDLLRHGRGPVFRLAAEIALHH
HECWDGSGYPQGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQLEPRLVQHFHEALPQILQIK
DAWDSPVA
>Mature_327_residues
PSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVSRHAPKLILLDVELPDMDGYAVARALKQQPSSNAIPILF
VTSRNSEHDERLGLEAGAADYVSKPYSPALLKARIGTQLKLAENARLAQQYRDAIHLLGTAGQGQDADTGAHIWRMAVYA
RVLAEAVGWSPQQAQTLQDAAPLHDAGKIAVPGSILQKPDALDEHERSVVRQHPQAGHDLLRHGRGPVFRLAAEIALHHH
ECWDGSGYPQGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQLEPRLVQHFHEALPQILQIKD
AWDSPVA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=114, Percent_Identity=40.3508771929825, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790863, Length=227, Percent_Identity=27.3127753303965, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788394, Length=131, Percent_Identity=38.9312977099237, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=37.1900826446281, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786599, Length=124, Percent_Identity=34.6774193548387, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=36.7521367521368, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787229, Length=126, Percent_Identity=32.5396825396825, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 35750; Mature: 35619

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVSRHAPKLILLDVELPDMD
CCCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCC
GYAVARALKQQPSSNAIPILFVTSRNSEHDERLGLEAGAADYVSKPYSPALLKARIGTQL
HHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCEE
KLAENARLAQQYRDAIHLLGTAGQGQDADTGAHIWRMAVYARVLAEAVGWSPQQAQTLQD
EHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAPLHDAGKIAVPGSILQKPDALDEHERSVVRQHPQAGHDLLRHGRGPVFRLAAEIALHH
HCCCCCCCCEECCHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHH
HECWDGSGYPQGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQ
HHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
LEPRLVQHFHEALPQILQIKDAWDSPVA
CCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVSRHAPKLILLDVELPDMD
CCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCC
GYAVARALKQQPSSNAIPILFVTSRNSEHDERLGLEAGAADYVSKPYSPALLKARIGTQL
HHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCEE
KLAENARLAQQYRDAIHLLGTAGQGQDADTGAHIWRMAVYARVLAEAVGWSPQQAQTLQD
EHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAPLHDAGKIAVPGSILQKPDALDEHERSVVRQHPQAGHDLLRHGRGPVFRLAAEIALHH
HCCCCCCCCEECCHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHH
HECWDGSGYPQGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQ
HHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
LEPRLVQHFHEALPQILQIKDAWDSPVA
CCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]