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The map label for this gene is topB [H]

Identifier: 21242947

GI number: 21242947

Start: 2596003

End: 2598039

Strand: Direct

Name: topB [H]

Synonym: XAC2212

Alternate gene names: 21242947

Gene position: 2596003-2598039 (Clockwise)

Preceding gene: 21242946

Following gene: 21242948

Centisome position: 50.16

GC content: 68.58

Gene sequence:

>2037_bases
ATGCGCGTGGTTCTGTGCGAGAAGCCGTCCCAGGGCAAGGACATCGCCCGGGTCCTGGGCGCACACCGGCGAGAAGCCGG
CTGCCTGGGTGGGCCGGGCATCACAGTCACCTGGGGCAGCGGCCACCTGATCGAAGCCGCGCCCCCGGAGGCCTACGGCG
AGCACTTCCAGCGCTGGACGCTGCCGATCGTTCCCCCGCGGTGGCAGTGGGTCGTCAAGGCACGCAGCGCCGCGCAGTTC
AAGGTCGTCAAACGGCTGCTCGGCGAGGCCTCCGAGCTCATCATCGCCACCGACGCCGATCGCGAAGGCGAGATGATCGC
CCGCGAGATGATCGACTACTGCGGCTACCGCGGCCCGGTCCAGCGTCTGTGGCTGTCGGCGCTCAACGATGCGTCGATCC
GCAAGGCGTTCGGCGCACTGCGGGCCTCCAGCGAGACGCTCTCCCTCTACCACTGCGCGCTCGCGCGTTCCCGCGCCGAC
TGGCTGGTCGGCATCAATCTGACCCGCCTGTTCACGCTGCTCGGGCGCCATGCCGGCCACGACGGCGTGCTCTCCGTGGG
GCGCGTGCAGACGCCCACCCTCAAACTGGTCGTGGACCGCGACCGGGAGATCGCGGCATTCGTGCCGGTTCCGTTCTGGT
CCATCGATGTGTCTCTTTCCGCGGCAAATGTGCCGTTCTCCGCCCATTGGCTTGCGCCCCGCACCGCAACGGACGATGCG
GGACGCTGTCTGCGGCAGTCCGTCGCACGCCATGCGGCCGACGCCATCCGCGCCGCCGGCACCGCGCAGGTCGTGCACAC
CGAGACCGAGCGCGTGCGCGAAGGCCCGCCCCTTCCCTTCGATCTGAGCACGTTGCAGGAAGTCTGTTCACGGCGCCTCG
GCCTGGAGGTGCAGGAAACCCTCGACATCGCGCAAGCCCTCTACGAGACGCACAAGGCGACGACGTATCCCCGCACCGAT
GCGGGTTACCTGCCCGAGAACATGCTCGCGGAGGTTCCCACGGTCATCCAAAGTCTGGTGAAGACCGACCCCGCGCTGCA
GCCGCTGATCGACCGCCTCGACTTCCGCCAGCGTTCCCGGGCCTGGAACGATGCGAAGATCACCGCGCACCACGCCATCA
TTCCCACGCTGGAACCTGCCGATATTTCTGCGATGTCCGCGAAGGAGCTTGCGGTGTACCGGCTCATCCGCGCCCACTAT
CTCGCGCAGTTCCTTCCCCATCACGAGTTCGACCGAACCGTCGTGCGCTTCCGATGCGGCGGCGAGACGCTGCAGGCGGT
CGGCAAGCGCATCGCCGTTCCGGGCTGGCAGCACGCATTCTCGGCACCGTCGCTGCCGCACGTCGCGGCCGATGGGGCTG
ACACCGAAGGCAGCGACGAAGCGCCCCGCGAGCAGATGCTGCCGGTACTGCAGGAAGGCATATCGTGTCAGGTGAGCGCG
CTGGATCTGAAGTCGCTGAGCACCAAGCCGCCACGGCCCTATACCCAGGGCGAACTCGTCAAGGCGATGAAGACGGTCGC
CCGGCTCGTGACCGATCCGCGCCTCAAGCAAAAGCTCAAGGACACCACCGGCATCGGCACGGAGGCAACGCGCGCGAACA
TCATCAGCGGGCTGCTTTCCCGGGGATTCCTGATCCGCAAGGGCCGCGCCGTACGGGCATCGGACACGGCGATGATGCTT
ATCGACGCCGTTCCCGCGGCCATCGCGGACCCGGGCACCACGGCGATCTGGGAGCAGGCCCTGGACATGATCGAAGCGCG
CCAGATGGGACTGGAGTCCTTCGTCGAGAAGCAGGCCGCCTGGGTCACGCAGCTGGTGCGGCAGCATCTCGGCACCACCC
TCGCGATACGCAGTCCCGAGGGCCCGCCTTGCCCGCTGTGCGGCGCGGCGACGCGCCAGCGCACTGGCAGGAGCGGGCCG
TTCTGGTCCTGCAGCCGCTACCCGGACTGCAAGGGAACGGCCCCGGTCGCACCCGCGCAAGCGGGCCCGGACGGGCAGAA
GCGATCCAGGAGCCGCGGCGCTCGCGCGCAACGCTAG

Upstream 100 bases:

>100_bases
CGGAGCCTGCCGCATGCGCGTTGTTGGCGGCGCCTCCGAGGCATCCCCGCGATGCTGACGGCCATCTGATAACCGTCATG
CATTTCCTGGAGGTGGCTTC

Downstream 100 bases:

>100_bases
CCCCTCCCTCTGGCGTCCTTACTCCCGTATCGCGTTTCAAGCTTTCCCGCACGCACCCCGGGTGCGGCACACCCTCGCCC
ACCCGCGAGGTCCCCCAGCA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MRVVLCEKPSQGKDIARVLGAHRREAGCLGGPGITVTWGSGHLIEAAPPEAYGEHFQRWTLPIVPPRWQWVVKARSAAQF
KVVKRLLGEASELIIATDADREGEMIAREMIDYCGYRGPVQRLWLSALNDASIRKAFGALRASSETLSLYHCALARSRAD
WLVGINLTRLFTLLGRHAGHDGVLSVGRVQTPTLKLVVDRDREIAAFVPVPFWSIDVSLSAANVPFSAHWLAPRTATDDA
GRCLRQSVARHAADAIRAAGTAQVVHTETERVREGPPLPFDLSTLQEVCSRRLGLEVQETLDIAQALYETHKATTYPRTD
AGYLPENMLAEVPTVIQSLVKTDPALQPLIDRLDFRQRSRAWNDAKITAHHAIIPTLEPADISAMSAKELAVYRLIRAHY
LAQFLPHHEFDRTVVRFRCGGETLQAVGKRIAVPGWQHAFSAPSLPHVAADGADTEGSDEAPREQMLPVLQEGISCQVSA
LDLKSLSTKPPRPYTQGELVKAMKTVARLVTDPRLKQKLKDTTGIGTEATRANIISGLLSRGFLIRKGRAVRASDTAMML
IDAVPAAIADPGTTAIWEQALDMIEARQMGLESFVEKQAAWVTQLVRQHLGTTLAIRSPEGPPCPLCGAATRQRTGRSGP
FWSCSRYPDCKGTAPVAPAQAGPDGQKRSRSRGARAQR

Sequences:

>Translated_678_residues
MRVVLCEKPSQGKDIARVLGAHRREAGCLGGPGITVTWGSGHLIEAAPPEAYGEHFQRWTLPIVPPRWQWVVKARSAAQF
KVVKRLLGEASELIIATDADREGEMIAREMIDYCGYRGPVQRLWLSALNDASIRKAFGALRASSETLSLYHCALARSRAD
WLVGINLTRLFTLLGRHAGHDGVLSVGRVQTPTLKLVVDRDREIAAFVPVPFWSIDVSLSAANVPFSAHWLAPRTATDDA
GRCLRQSVARHAADAIRAAGTAQVVHTETERVREGPPLPFDLSTLQEVCSRRLGLEVQETLDIAQALYETHKATTYPRTD
AGYLPENMLAEVPTVIQSLVKTDPALQPLIDRLDFRQRSRAWNDAKITAHHAIIPTLEPADISAMSAKELAVYRLIRAHY
LAQFLPHHEFDRTVVRFRCGGETLQAVGKRIAVPGWQHAFSAPSLPHVAADGADTEGSDEAPREQMLPVLQEGISCQVSA
LDLKSLSTKPPRPYTQGELVKAMKTVARLVTDPRLKQKLKDTTGIGTEATRANIISGLLSRGFLIRKGRAVRASDTAMML
IDAVPAAIADPGTTAIWEQALDMIEARQMGLESFVEKQAAWVTQLVRQHLGTTLAIRSPEGPPCPLCGAATRQRTGRSGP
FWSCSRYPDCKGTAPVAPAQAGPDGQKRSRSRGARAQR
>Mature_678_residues
MRVVLCEKPSQGKDIARVLGAHRREAGCLGGPGITVTWGSGHLIEAAPPEAYGEHFQRWTLPIVPPRWQWVVKARSAAQF
KVVKRLLGEASELIIATDADREGEMIAREMIDYCGYRGPVQRLWLSALNDASIRKAFGALRASSETLSLYHCALARSRAD
WLVGINLTRLFTLLGRHAGHDGVLSVGRVQTPTLKLVVDRDREIAAFVPVPFWSIDVSLSAANVPFSAHWLAPRTATDDA
GRCLRQSVARHAADAIRAAGTAQVVHTETERVREGPPLPFDLSTLQEVCSRRLGLEVQETLDIAQALYETHKATTYPRTD
AGYLPENMLAEVPTVIQSLVKTDPALQPLIDRLDFRQRSRAWNDAKITAHHAIIPTLEPADISAMSAKELAVYRLIRAHY
LAQFLPHHEFDRTVVRFRCGGETLQAVGKRIAVPGWQHAFSAPSLPHVAADGADTEGSDEAPREQMLPVLQEGISCQVSA
LDLKSLSTKPPRPYTQGELVKAMKTVARLVTDPRLKQKLKDTTGIGTEATRANIISGLLSRGFLIRKGRAVRASDTAMML
IDAVPAAIADPGTTAIWEQALDMIEARQMGLESFVEKQAAWVTQLVRQHLGTTLAIRSPEGPPCPLCGAATRQRTGRSGP
FWSCSRYPDCKGTAPVAPAQAGPDGQKRSRSRGARAQR

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=714, Percent_Identity=24.7899159663866, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI4507635, Length=593, Percent_Identity=22.9342327150084, Blast_Score=113, Evalue=5e-25,
Organism=Escherichia coli, GI1788061, Length=662, Percent_Identity=38.9728096676737, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1787529, Length=605, Percent_Identity=25.9504132231405, Blast_Score=139, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=578, Percent_Identity=24.5674740484429, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=569, Percent_Identity=23.5500878734622, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=505, Percent_Identity=26.5346534653465, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24585251, Length=693, Percent_Identity=24.3867243867244, Blast_Score=120, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24640096, Length=480, Percent_Identity=23.3333333333333, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 74165; Mature: 74165

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVLCEKPSQGKDIARVLGAHRREAGCLGGPGITVTWGSGHLIEAAPPEAYGEHFQRWT
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHCC
LPIVPPRWQWVVKARSAAQFKVVKRLLGEASELIIATDADREGEMIAREMIDYCGYRGPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHH
QRLWLSALNDASIRKAFGALRASSETLSLYHCALARSRADWLVGINLTRLFTLLGRHAGH
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCC
DGVLSVGRVQTPTLKLVVDRDREIAAFVPVPFWSIDVSLSAANVPFSAHWLAPRTATDDA
CCCEECCCCCCCEEEEEEECCCCEEEEECCCEEEEEEEEEECCCCEEEEECCCCCCCCHH
GRCLRQSVARHAADAIRAAGTAQVVHTETERVREGPPLPFDLSTLQEVCSRRLGLEVQET
HHHHHHHHHHHHHHHHHHCCCHHEEECHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHH
LDIAQALYETHKATTYPRTDAGYLPENMLAEVPTVIQSLVKTDPALQPLIDRLDFRQRSR
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
AWNDAKITAHHAIIPTLEPADISAMSAKELAVYRLIRAHYLAQFLPHHEFDRTVVRFRCG
CCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GETLQAVGKRIAVPGWQHAFSAPSLPHVAADGADTEGSDEAPREQMLPVLQEGISCQVSA
CHHHHHHHHHCCCCCHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEE
LDLKSLSTKPPRPYTQGELVKAMKTVARLVTDPRLKQKLKDTTGIGTEATRANIISGLLS
EEHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RGFLIRKGRAVRASDTAMMLIDAVPAAIADPGTTAIWEQALDMIEARQMGLESFVEKQAA
CCEEEECCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVTQLVRQHLGTTLAIRSPEGPPCPLCGAATRQRTGRSGPFWSCSRYPDCKGTAPVAPAQ
HHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGPDGQKRSRSRGARAQR
CCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MRVVLCEKPSQGKDIARVLGAHRREAGCLGGPGITVTWGSGHLIEAAPPEAYGEHFQRWT
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHCC
LPIVPPRWQWVVKARSAAQFKVVKRLLGEASELIIATDADREGEMIAREMIDYCGYRGPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHH
QRLWLSALNDASIRKAFGALRASSETLSLYHCALARSRADWLVGINLTRLFTLLGRHAGH
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCC
DGVLSVGRVQTPTLKLVVDRDREIAAFVPVPFWSIDVSLSAANVPFSAHWLAPRTATDDA
CCCEECCCCCCCEEEEEEECCCCEEEEECCCEEEEEEEEEECCCCEEEEECCCCCCCCHH
GRCLRQSVARHAADAIRAAGTAQVVHTETERVREGPPLPFDLSTLQEVCSRRLGLEVQET
HHHHHHHHHHHHHHHHHHCCCHHEEECHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHH
LDIAQALYETHKATTYPRTDAGYLPENMLAEVPTVIQSLVKTDPALQPLIDRLDFRQRSR
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
AWNDAKITAHHAIIPTLEPADISAMSAKELAVYRLIRAHYLAQFLPHHEFDRTVVRFRCG
CCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GETLQAVGKRIAVPGWQHAFSAPSLPHVAADGADTEGSDEAPREQMLPVLQEGISCQVSA
CHHHHHHHHHCCCCCHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEE
LDLKSLSTKPPRPYTQGELVKAMKTVARLVTDPRLKQKLKDTTGIGTEATRANIISGLLS
EEHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RGFLIRKGRAVRASDTAMMLIDAVPAAIADPGTTAIWEQALDMIEARQMGLESFVEKQAA
CCEEEECCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVTQLVRQHLGTTLAIRSPEGPPCPLCGAATRQRTGRSGPFWSCSRYPDCKGTAPVAPAQ
HHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGPDGQKRSRSRGARAQR
CCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]