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The map label for this gene is cheB

Identifier: 21242632

GI number: 21242632

Start: 2195047

End: 2196123

Strand: Reverse

Name: cheB

Synonym: XAC1888

Alternate gene names: 21242632

Gene position: 2196123-2195047 (Counterclockwise)

Preceding gene: 21242633

Following gene: 77748615

Centisome position: 42.43

GC content: 66.02

Gene sequence:

>1077_bases
ATGACGCTGGAAACTCCTGTGCGTGTATTGATCGTCGACGATTCGGCGGTCGTGCGTCAGATGCTCACCGAAATTTTGTC
GCGCGACGCCGGCATCGAAGTGGTCGGCTCGGCCGCCGACCCGTTGCTGGCGCGCGAAAAGATCAAGCGCCTCAATCCAG
ACGTCATCACGCTGGATGTGGAAATGCCGCGCATGGACGGCCTGGTGTTTCTGGAAAACCTGATGCGCCTGCGCCCTACC
CCGGTGGTGATGATTTCATCGCTGACCGAGCGCGGCGCCGACACCACCTTGCAGGCTTTGTCGCTGGGCGCCGTGGACTT
CGTGTCCAAGCCCAAGATCGACGTTGCACGCGGGCTGGAAGGCTACGCCGAAGAGATCGTCAGCAAGGTCAAAATGGCCG
CCAAGGCCAAGGTCAGCGCACTCAATCGCCCGAGCGCGCCCAAAGTCACGCTGGATATGCAATCGGCACCGGTGGCCGGC
AGCGCGCTGCGCTTTCGCACGACCGACCGTTTGATCGCCATCGGCGCCTCCGCCGGTGGTACCGAAGCGCTGCGCGTGGT
GCTCGAACACATGCCCGCCGATGCGCCCGCGGTGGTGATGACCCAGCATCTGCCTGCCAGCTTCAGCACCGCATTCGCCG
AGCGCCTCAACCGCCATTCGGCCATGTCGGTACGCGAAGCCACCGATGGCGAGGCGATCCTGCCAGGTCATGCGTATCTG
CCGCCGGGCGGCCAGCATCTGCGCATCATTCGCGACGGAGCGCGTTGGCGCTGCCGTATCGACGACGGCCCGCCGGTCAA
TCGCCACAAACCGGCAGTGGACGTGCTGTTTCGTTCCGTTGCCGCCAATGCCGGCCCGAATGCGGTGGGCGCCATCCTCA
CCGGCATGGGCGACGACGGCGCACGCGGCCTGCTGGAAATGCTGCAGGCCGGCGCGCCGACGCTGGTGCAGGACGAAGCC
AGCAGCGTGGTCTGGGGCATGCCCGGTGCGGCGTACAAGCTTGGCGCCGCACAGGAAGTGGTGCCGCTGGACCGCGTAGC
CGAGCGCTTGCTCGCATTGTCGGCGCAGGCGCGCTGA

Upstream 100 bases:

>100_bases
GTTTGCCGCACGCACACGAAGCCGAAGTCGCCGCCACCGAATCGGCCGTGCGCGCCCGTCTGTCCAAAGCACCGGTCACC
GGTGGAGTGGAGTTGTTCGA

Downstream 100 bases:

>100_bases
TCCGGCATGGCCGCGCATCCCTCGCACCCGACCAACCAAACGCTTCCTCCAGCACCGGGAAGCGCGGCGGGCGCTGGCGT
GGATGCAGCGATGGACGGCA

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTLETPVRVLIVDDSAVVRQMLTEILSRDAGIEVVGSAADPLLAREKIKRLNPDVITLDVEMPRMDGLVFLENLMRLRPT
PVVMISSLTERGADTTLQALSLGAVDFVSKPKIDVARGLEGYAEEIVSKVKMAAKAKVSALNRPSAPKVTLDMQSAPVAG
SALRFRTTDRLIAIGASAGGTEALRVVLEHMPADAPAVVMTQHLPASFSTAFAERLNRHSAMSVREATDGEAILPGHAYL
PPGGQHLRIIRDGARWRCRIDDGPPVNRHKPAVDVLFRSVAANAGPNAVGAILTGMGDDGARGLLEMLQAGAPTLVQDEA
SSVVWGMPGAAYKLGAAQEVVPLDRVAERLLALSAQAR

Sequences:

>Translated_358_residues
MTLETPVRVLIVDDSAVVRQMLTEILSRDAGIEVVGSAADPLLAREKIKRLNPDVITLDVEMPRMDGLVFLENLMRLRPT
PVVMISSLTERGADTTLQALSLGAVDFVSKPKIDVARGLEGYAEEIVSKVKMAAKAKVSALNRPSAPKVTLDMQSAPVAG
SALRFRTTDRLIAIGASAGGTEALRVVLEHMPADAPAVVMTQHLPASFSTAFAERLNRHSAMSVREATDGEAILPGHAYL
PPGGQHLRIIRDGARWRCRIDDGPPVNRHKPAVDVLFRSVAANAGPNAVGAILTGMGDDGARGLLEMLQAGAPTLVQDEA
SSVVWGMPGAAYKLGAAQEVVPLDRVAERLLALSAQAR
>Mature_357_residues
TLETPVRVLIVDDSAVVRQMLTEILSRDAGIEVVGSAADPLLAREKIKRLNPDVITLDVEMPRMDGLVFLENLMRLRPTP
VVMISSLTERGADTTLQALSLGAVDFVSKPKIDVARGLEGYAEEIVSKVKMAAKAKVSALNRPSAPKVTLDMQSAPVAGS
ALRFRTTDRLIAIGASAGGTEALRVVLEHMPADAPAVVMTQHLPASFSTAFAERLNRHSAMSVREATDGEAILPGHAYLP
PGGQHLRIIRDGARWRCRIDDGPPVNRHKPAVDVLFRSVAANAGPNAVGAILTGMGDDGARGLLEMLQAGAPTLVQDEAS
SVVWGMPGAAYKLGAAQEVVPLDRVAERLLALSAQAR

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=348, Percent_Identity=54.5977011494253, Blast_Score=381, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_XANAC (Q8PLB4)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642214.1
- ProteinModelPortal:   Q8PLB4
- SMR:   Q8PLB4
- GeneID:   1155959
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1888
- HOGENOM:   HBG705324
- OMA:   PPSVADF
- ProtClustDB:   PRK12555
- BioCyc:   XAXO190486:XAC1888-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38029; Mature: 37897

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 177-177 ACT_SITE 203-203 ACT_SITE 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLETPVRVLIVDDSAVVRQMLTEILSRDAGIEVVGSAADPLLAREKIKRLNPDVITLDV
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEE
EMPRMDGLVFLENLMRLRPTPVVMISSLTERGADTTLQALSLGAVDFVSKPKIDVARGLE
CCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCCHHHHHHHHCHHHHCCCCCHHHHHCCH
GYAEEIVSKVKMAAKAKVSALNRPSAPKVTLDMQSAPVAGSALRFRTTDRLIAIGASAGG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCEEEEEECCCCC
TEALRVVLEHMPADAPAVVMTQHLPASFSTAFAERLNRHSAMSVREATDGEAILPGHAYL
HHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
PPGGQHLRIIRDGARWRCRIDDGPPVNRHKPAVDVLFRSVAANAGPNAVGAILTGMGDDG
CCCCCEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCH
ARGLLEMLQAGAPTLVQDEASSVVWGMPGAAYKLGAAQEVVPLDRVAERLLALSAQAR
HHHHHHHHHCCCCCEECCCCCCEEECCCCCCEECCCCHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLETPVRVLIVDDSAVVRQMLTEILSRDAGIEVVGSAADPLLAREKIKRLNPDVITLDV
CCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEE
EMPRMDGLVFLENLMRLRPTPVVMISSLTERGADTTLQALSLGAVDFVSKPKIDVARGLE
CCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCCHHHHHHHHCHHHHCCCCCHHHHHCCH
GYAEEIVSKVKMAAKAKVSALNRPSAPKVTLDMQSAPVAGSALRFRTTDRLIAIGASAGG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCEEEEEECCCCC
TEALRVVLEHMPADAPAVVMTQHLPASFSTAFAERLNRHSAMSVREATDGEAILPGHAYL
HHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
PPGGQHLRIIRDGARWRCRIDDGPPVNRHKPAVDVLFRSVAANAGPNAVGAILTGMGDDG
CCCCCEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCH
ARGLLEMLQAGAPTLVQDEASSVVWGMPGAAYKLGAAQEVVPLDRVAERLLALSAQAR
HHHHHHHHHCCCCCEECCCCCCEEECCCCCCEECCCCHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217