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The map label for this gene is pdeA [H]

Identifier: 77748615

GI number: 77748615

Start: 2192683

End: 2194869

Strand: Reverse

Name: pdeA [H]

Synonym: XAC1887

Alternate gene names: 77748615

Gene position: 2194869-2192683 (Counterclockwise)

Preceding gene: 21242632

Following gene: 21242626

Centisome position: 42.41

GC content: 64.61

Gene sequence:

>2187_bases
ATGGCCAAGGCCGGCGAGCAGCCCGAGCAGCGCCGTTCGCGCCTGCTCGCAGAAGCCCTCGATGGCAGCAGCAGCGCCAT
TCTCATCTGCGATCTGCAATCGCGCCTGCTGTACGCCAACGACGGCTTCCAGCGCATGTTCGGCTACACCGAAGCCGAGC
TGGTCGGTCTGCGTCCCTCCGATGTCTTGACCCGCGCCCAAAGCGACCAAAGCGAGGTCGCCCGCGTTCGCGATCGCGCC
GATGCCTTGCAGCACACCCGCGCCGACTTGCTGGTCTATCGCAAGGACGGCACACCGCTGTGGATCTCGCTCAACTTCAA
CCCGATCTACGACGCCAACCACGCTCCCTCGCATTACGTGGCGGTATTGACCGACATCACCGACACCAAGATGCACGAGG
TATTGCAGCACCGCGTGCTGGAAGCCTTGGTGCAGGAACGCCCGTTGATCGAGGTGATGACGCTGATCTGCACCGAGGTC
GAACGCATCGCACCGGACGTGCTGGCCACGGTGATGAGTGCGGACAATCAAGGTCGCCTGCATTCGCTGGCTGCGCCCAG
CCTGCCGCCCGAATACGCCGATGCGGTCAACGGCTTGAAGATCGGCCCGGGCACAGGCGCCTGCGGCACCGCGGCCTGGC
GCGGTCGCGCGGTGATGGTGGAAGACATCGCCACCGACCCATTGTTCGAGGCGTATCGCGACACCCTCTTGCCTTGGGGC
CTGCGCGCCTGCTGGAGCACGCCGATCCGCTCCAACAGCGCGCGCGTGCTTGGCACCTTCGCGCTGTATTACCGCAAGCC
GCAACGTGCCGATGCCTGGCATATGCGGCTCACCGATTTGTGCCAGCAGCTGTGCACCTTGGCGCTGGAACGCGAGCAGA
TCCGCCAGCGCGTGCATCAACTGGCGTTCTACGACACGCTCACGGGCCTGCCCAACCGCATCATGTTCAGCGCGCGTGCC
GAGCAGGCGCTGACGCAGGCCGAATACGCAGGCGAACCGGCCGCGTTGATGTTCATCAACATCGATCGCTTCAAGCTCAT
CAACGATAGCCAGGGACACGCCGCAGGCGATGGGCTGCTGCGCGATATCGCCTTGCGCGTCAACGACCAGGTCGCCGCCA
CGGCGATGCTGGCGCGACTCTCCGGCGACGAATTCGCGCTTGCGCTGCCGCAATGCAGCGCAGAGCAGGCCAGTGCCGCC
GCCGAGCGTTTGTTGGTCAGCATCGCCAGCCCCCTGCCGGTCGGCCACATGACCGTGCATTCGTCGGCCAGTATCGGCGT
GGCGATGTTTCCCGAGGATGGCCAAGACATCAACATCCTGCTGCGCCACGCCGATCTGGCGATGAATCGCGCCAAGAAGG
AAGGCGGTGGGCGCTTCCGTTTCTTCAGTTCCGACATGAACCGCATGGCGCAAGAACGCGTTGCGCTGGAAGCGGAACTG
CGTCAGGCCATCGACCGCGATCAACTGCAATTGCATTACCAACCGCAGATGCACAGCGCCGCGCCGCACACGCTCTATGG
CGTGGAAGCGCTGCTGCGCTGGAATCACCCGCAGTTGGGTGCAGTGTCGCCGGCGCGCTTCCTGCCCGTGGCCGAAGAAC
AAGGCATGATGCCGCAGATCAACGCGTGGGTCCTGCGCAGCGCCTGCCAGCAGGTCGCCGACTGGCGCCAGCGTGGCGTG
CCGGTCGCACGCGTGTCGGTGAACCTGTCGGCGAGTGGTTTCGAAAGTCCGCGCATGCTGTCGGATCTGCTCCGGCTCAT
CGCCGAGACGGGCGTACAGCCCTCCGATCTGACCCTGGAACTGACCGAAAGCGTGATGCTGTCCGGGCAACCGGGCGTAT
TGGAAAACCTGCATGCCATTCGCGAAGCCGGCATCTCGCTGTCGCTGGACGATTTCGGCACCGGCTATTCCAGCCTGAGC
CATCTGCATCGCCTGCCGATCGACGAACTCAAATTGGACATGAGCTTCGTCCGCGATATCGAACACAGCGAAGACGCGCG
CGCGCTCACCACCTCGGTATTGCGCATCGGCGAGCACCTGCGCAAGCACGTGGTCGCCGAAGGTGTGGAAAACGAAGCGC
AGCGCCTGTTCCTCGCCGACCTGGGCTGTGAGGTGCTGCAAGGCTATCTGTTCTCGCGGCCCCTACCCGCCCCGGCACTG
GAAGCCTGGCTGGACGCGCAACCGTAA

Upstream 100 bases:

>100_bases
CGTGGATGCAGCGATGGACGGCATGCCGCTGACGGCTGAGCCAATCCATGCCAACGCCCCAAGCGCTGGTCTGGTCAGGC
CCGGCCCGGTCACCGCCGGC

Downstream 100 bases:

>100_bases
TCGCGCAGCTTCGAACTCCCTTCCAGCCAGGAAAGGCGGGCCATGGCGTGACAAGGAACGAGCCTCCCTGCTGCAGGAGA
GCAGCGCTGCAAGCCCTTCC

Product: c-di-GMP phosphodiesterase A

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MAKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYANDGFQRMFGYTEAELVGLRPSDVLTRAQSDQSEVARVRDRA
DALQHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYVAVLTDITDTKMHEVLQHRVLEALVQERPLIEVMTLICTEV
ERIAPDVLATVMSADNQGRLHSLAAPSLPPEYADAVNGLKIGPGTGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWG
LRACWSTPIRSNSARVLGTFALYYRKPQRADAWHMRLTDLCQQLCTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARA
EQALTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLLRDIALRVNDQVAATAMLARLSGDEFALALPQCSAEQASAA
AERLLVSIASPLPVGHMTVHSSASIGVAMFPEDGQDINILLRHADLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAEL
RQAIDRDQLQLHYQPQMHSAAPHTLYGVEALLRWNHPQLGAVSPARFLPVAEEQGMMPQINAWVLRSACQQVADWRQRGV
PVARVSVNLSASGFESPRMLSDLLRLIAETGVQPSDLTLELTESVMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLS
HLHRLPIDELKLDMSFVRDIEHSEDARALTTSVLRIGEHLRKHVVAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAPAL
EAWLDAQP

Sequences:

>Translated_728_residues
MAKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYANDGFQRMFGYTEAELVGLRPSDVLTRAQSDQSEVARVRDRA
DALQHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYVAVLTDITDTKMHEVLQHRVLEALVQERPLIEVMTLICTEV
ERIAPDVLATVMSADNQGRLHSLAAPSLPPEYADAVNGLKIGPGTGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWG
LRACWSTPIRSNSARVLGTFALYYRKPQRADAWHMRLTDLCQQLCTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARA
EQALTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLLRDIALRVNDQVAATAMLARLSGDEFALALPQCSAEQASAA
AERLLVSIASPLPVGHMTVHSSASIGVAMFPEDGQDINILLRHADLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAEL
RQAIDRDQLQLHYQPQMHSAAPHTLYGVEALLRWNHPQLGAVSPARFLPVAEEQGMMPQINAWVLRSACQQVADWRQRGV
PVARVSVNLSASGFESPRMLSDLLRLIAETGVQPSDLTLELTESVMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLS
HLHRLPIDELKLDMSFVRDIEHSEDARALTTSVLRIGEHLRKHVVAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAPAL
EAWLDAQP
>Mature_727_residues
AKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYANDGFQRMFGYTEAELVGLRPSDVLTRAQSDQSEVARVRDRAD
ALQHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYVAVLTDITDTKMHEVLQHRVLEALVQERPLIEVMTLICTEVE
RIAPDVLATVMSADNQGRLHSLAAPSLPPEYADAVNGLKIGPGTGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWGL
RACWSTPIRSNSARVLGTFALYYRKPQRADAWHMRLTDLCQQLCTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARAE
QALTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLLRDIALRVNDQVAATAMLARLSGDEFALALPQCSAEQASAAA
ERLLVSIASPLPVGHMTVHSSASIGVAMFPEDGQDINILLRHADLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAELR
QAIDRDQLQLHYQPQMHSAAPHTLYGVEALLRWNHPQLGAVSPARFLPVAEEQGMMPQINAWVLRSACQQVADWRQRGVP
VARVSVNLSASGFESPRMLSDLLRLIAETGVQPSDLTLELTESVMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLSH
LHRLPIDELKLDMSFVRDIEHSEDARALTTSVLRIGEHLRKHVVAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAPALE
AWLDAQP

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=721, Percent_Identity=29.126213592233, Blast_Score=340, Evalue=2e-94,
Organism=Escherichia coli, GI1787541, Length=434, Percent_Identity=36.405529953917, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI226510982, Length=451, Percent_Identity=29.9334811529933, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1790496, Length=264, Percent_Identity=31.8181818181818, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87081845, Length=263, Percent_Identity=31.5589353612167, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87081743, Length=244, Percent_Identity=34.0163934426229, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788502, Length=249, Percent_Identity=31.7269076305221, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788849, Length=469, Percent_Identity=26.0127931769723, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1787055, Length=366, Percent_Identity=24.5901639344262, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI87081980, Length=263, Percent_Identity=32.319391634981, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1786507, Length=242, Percent_Identity=30.5785123966942, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788381, Length=431, Percent_Identity=25.5220417633411, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87082096, Length=247, Percent_Identity=29.1497975708502, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI145693134, Length=195, Percent_Identity=29.7435897435897, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=32.9479768786127, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI87081977, Length=225, Percent_Identity=27.5555555555556, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=33.5329341317365, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789650, Length=474, Percent_Identity=21.3080168776371, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=32.9192546583851, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=197, Percent_Identity=28.9340101522843, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787262, Length=269, Percent_Identity=25.2788104089219, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787410, Length=150, Percent_Identity=28, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=134, Percent_Identity=36.5671641791045, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=28.0487804878049, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 80476; Mature: 80345

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYANDGFQRMFGYTEAELVGLRPS
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCHHHHHCCCCCEEECCCCH
DVLTRAQSDQSEVARVRDRADALQHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYV
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCEE
AVLTDITDTKMHEVLQHRVLEALVQERPLIEVMTLICTEVERIAPDVLATVMSADNQGRL
EEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HSLAAPSLPPEYADAVNGLKIGPGTGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWG
CCCCCCCCCHHHHHHCCCCEECCCCCCCCCHHHCCCEEEEEHHCCCHHHHHHHHHHCCCH
LRACWSTPIRSNSARVLGTFALYYRKPQRADAWHMRLTDLCQQLCTLALEREQIRQRVHQ
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLL
HHHHHHHHCCCHHHEEHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCHHHHH
RDIALRVNDQVAATAMLARLSGDEFALALPQCSAEQASAAAERLLVSIASPLPVGHMTVH
HHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
SSASIGVAMFPEDGQDINILLRHADLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAEL
CCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
RQAIDRDQLQLHYQPQMHSAAPHTLYGVEALLRWNHPQLGAVSPARFLPVAEEQGMMPQI
HHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHCCCCCCHH
NAWVLRSACQQVADWRQRGVPVARVSVNLSASGFESPRMLSDLLRLIAETGVQPSDLTLE
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCE
LTESVMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDI
EHHHHHCCCCCCHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHC
EHSEDARALTTSVLRIGEHLRKHVVAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAPAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
EAWLDAQP
HHHCCCCC
>Mature Secondary Structure 
AKAGEQPEQRRSRLLAEALDGSSSAILICDLQSRLLYANDGFQRMFGYTEAELVGLRPS
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCHHHHHCCCCCEEECCCCH
DVLTRAQSDQSEVARVRDRADALQHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYV
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCEE
AVLTDITDTKMHEVLQHRVLEALVQERPLIEVMTLICTEVERIAPDVLATVMSADNQGRL
EEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HSLAAPSLPPEYADAVNGLKIGPGTGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWG
CCCCCCCCCHHHHHHCCCCEECCCCCCCCCHHHCCCEEEEEHHCCCHHHHHHHHHHCCCH
LRACWSTPIRSNSARVLGTFALYYRKPQRADAWHMRLTDLCQQLCTLALEREQIRQRVHQ
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLL
HHHHHHHHCCCHHHEEHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCHHHHH
RDIALRVNDQVAATAMLARLSGDEFALALPQCSAEQASAAAERLLVSIASPLPVGHMTVH
HHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
SSASIGVAMFPEDGQDINILLRHADLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAEL
CCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
RQAIDRDQLQLHYQPQMHSAAPHTLYGVEALLRWNHPQLGAVSPARFLPVAEEQGMMPQI
HHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHCCCCCCHH
NAWVLRSACQQVADWRQRGVPVARVSVNLSASGFESPRMLSDLLRLIAETGVQPSDLTLE
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCE
LTESVMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDI
EHHHHHCCCCCCHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHC
EHSEDARALTTSVLRIGEHLRKHVVAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAPAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
EAWLDAQP
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]