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The map label for this gene is rpfF [H]

Identifier: 21242623

GI number: 21242623

Start: 2181541

End: 2182410

Strand: Reverse

Name: rpfF [H]

Synonym: XAC1879

Alternate gene names: 21242623

Gene position: 2182410-2181541 (Counterclockwise)

Preceding gene: 21242624

Following gene: 21242617

Centisome position: 42.17

GC content: 62.53

Gene sequence:

>870_bases
ATGAACACGATTGAAAAGATTTCCCTCGGCCGCCCCCACTCCACCATTCGTGTTGATTCGTCGGCCGTCGACGGTACCCA
TTGGCTGTTCATGCATGCCGATGCGCGGGTCGGCGTGCGCCCTTGCTGTCGCCCCGAACTGCTGGAAGAAATGTGGGATG
TCATGGGCTCCATCACCCGCGCTCCGCACGAGCGCCGCAGTGGCGAACTACGCCACTTCATCCTCGCCTCCGATTCGAGC
GCCTTCAATCTCGGCGGGGATCTGGACCTGTTCGCACGGCTGATCCGCGAACAGAACCGCGAGCAGTTGCTGCGCTACGC
CAAGCGTTGCGTGGAAGGCGTGCACCATCTGCATGTCGGCTTCGGCGGCGACGTGCGCAGCATCGCCTTGCTGCAGGGCG
ATGCGCTGGGAGGCGGCCTGGAAATGGCATTGGCCTGCCACACCATCGTGGCCGAGGAAGGCATCGGCATGGGACTTCCC
GAAGTCATGTTCGGCCTGTTTCCCGGCATGGGCGCATATTCCTTCCTGTGCCGCCGCGTCGCCCCTCAACTGGCCGAGAA
AATCATTCTCGAAGGACGCATCTACAGCAGCGAAGAAATGCACGCGATGGGCATCGTCGACGTGCTGGTGCCGAAGGGAC
AGGGCGTCGTTGCGACCGAAAACCTGATCCGCCAGCAAAAGCGCATTTCGCACGCGTACCTGGCCATGAATGCGGCACGC
AATCTGGCGCAGTCGGTTCCCTACGAGGAACTGCTGCGCATCACCGAAGTGTGGGTGGACACGGCCCTGGCGCTCAATGA
AAAGTCGCTGCGAACGATGGACCGGTTGGTCAAGGCGCAGAACCGGCGCGTCGGCATGGGCGTCGCCTGA

Upstream 100 bases:

>100_bases
CGTTCATGGAGATAGTGCCGGTGTAGCATCGATCGGGAGTGGATCGTGCGTGCCCGCCGACATCCGCTCTCCCACCGCGG
GCCTTTCCAGGATCAAACCC

Downstream 100 bases:

>100_bases
TTTTTGATACGTCGCATCTTCAGGGGGAATCAGGCCCGGCCGGATGCCGGGCTTTACTTAATCAGGGATCGCCATTACCG
CTGGCCCCGCCCTCGGTCTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MNTIEKISLGRPHSTIRVDSSAVDGTHWLFMHADARVGVRPCCRPELLEEMWDVMGSITRAPHERRSGELRHFILASDSS
AFNLGGDLDLFARLIREQNREQLLRYAKRCVEGVHHLHVGFGGDVRSIALLQGDALGGGLEMALACHTIVAEEGIGMGLP
EVMFGLFPGMGAYSFLCRRVAPQLAEKIILEGRIYSSEEMHAMGIVDVLVPKGQGVVATENLIRQQKRISHAYLAMNAAR
NLAQSVPYEELLRITEVWVDTALALNEKSLRTMDRLVKAQNRRVGMGVA

Sequences:

>Translated_289_residues
MNTIEKISLGRPHSTIRVDSSAVDGTHWLFMHADARVGVRPCCRPELLEEMWDVMGSITRAPHERRSGELRHFILASDSS
AFNLGGDLDLFARLIREQNREQLLRYAKRCVEGVHHLHVGFGGDVRSIALLQGDALGGGLEMALACHTIVAEEGIGMGLP
EVMFGLFPGMGAYSFLCRRVAPQLAEKIILEGRIYSSEEMHAMGIVDVLVPKGQGVVATENLIRQQKRISHAYLAMNAAR
NLAQSVPYEELLRITEVWVDTALALNEKSLRTMDRLVKAQNRRVGMGVA
>Mature_289_residues
MNTIEKISLGRPHSTIRVDSSAVDGTHWLFMHADARVGVRPCCRPELLEEMWDVMGSITRAPHERRSGELRHFILASDSS
AFNLGGDLDLFARLIREQNREQLLRYAKRCVEGVHHLHVGFGGDVRSIALLQGDALGGGLEMALACHTIVAEEGIGMGLP
EVMFGLFPGMGAYSFLCRRVAPQLAEKIILEGRIYSSEEMHAMGIVDVLVPKGQGVVATENLIRQQKRISHAYLAMNAAR
NLAQSVPYEELLRITEVWVDTALALNEKSLRTMDRLVKAQNRRVGMGVA

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=30.3370786516854, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788682, Length=150, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787659, Length=113, Percent_Identity=35.3982300884956, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790281, Length=166, Percent_Identity=29.5180722891566, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653139, Length=233, Percent_Identity=29.1845493562232, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 32008; Mature: 32008

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIEKISLGRPHSTIRVDSSAVDGTHWLFMHADARVGVRPCCRPELLEEMWDVMGSITR
CCCHHHCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHC
APHERRSGELRHFILASDSSAFNLGGDLDLFARLIREQNREQLLRYAKRCVEGVHHLHVG
CCCHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECC
FGGDVRSIALLQGDALGGGLEMALACHTIVAEEGIGMGLPEVMFGLFPGMGAYSFLCRRV
CCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHH
APQLAEKIILEGRIYSSEEMHAMGIVDVLVPKGQGVVATENLIRQQKRISHAYLAMNAAR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
NLAQSVPYEELLRITEVWVDTALALNEKSLRTMDRLVKAQNRRVGMGVA
HHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNTIEKISLGRPHSTIRVDSSAVDGTHWLFMHADARVGVRPCCRPELLEEMWDVMGSITR
CCCHHHCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHC
APHERRSGELRHFILASDSSAFNLGGDLDLFARLIREQNREQLLRYAKRCVEGVHHLHVG
CCCHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECC
FGGDVRSIALLQGDALGGGLEMALACHTIVAEEGIGMGLPEVMFGLFPGMGAYSFLCRRV
CCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHH
APQLAEKIILEGRIYSSEEMHAMGIVDVLVPKGQGVVATENLIRQQKRISHAYLAMNAAR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
NLAQSVPYEELLRITEVWVDTALALNEKSLRTMDRLVKAQNRRVGMGVA
HHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA