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The map label for this gene is paaF [C]

Identifier: 21242597

GI number: 21242597

Start: 2148671

End: 2149468

Strand: Reverse

Name: paaF [C]

Synonym: XAC1853

Alternate gene names: 21242597

Gene position: 2149468-2148671 (Counterclockwise)

Preceding gene: 21242615

Following gene: 21242596

Centisome position: 41.53

GC content: 68.55

Gene sequence:

>798_bases
ATGCAGGACACCGGCTTGATTGTCGAAGACCACGGAGCGGTCCGCACCTTGCGATTGCATCGCCCCGCAGTCCACAACGC
CTTCGATGCGACGCTGATCGCCGCGCTGACCGATGCATTGCGCGAGGCTGGCCGGGCCTCGCAGATCCGCATCGTCGTGC
TGACCGGTGATGGCGCGGCCTTTTCGGCCGGCGCAGACCTGAACTGGATGCGCGGCATGGCCAGCGCCAACGAAGCGGAG
AACGCCGCCGACGCACTGGCGCTGGCGCAGTTGATGCGCACCCTGGATGAACTGCCCAAGCCCACCATCGCGCGCGTCAA
CGGGGCCGCCTTCGGCGGTGGTGTCGGCCTGGTGGCGTGTTGCGATATCGCCATCGGTTGTACCGAGGCGCGCTTCGGCC
TGACCGAGAGCAAGCTCGGCCTGCTGCCGGCGGTCATTTCGCCTTATGTGGTTGCAGCGATCGGTGCGCGCCAGGCGCGG
CGCTGGTTCGCAACCGCCGAGATCTTCGATGCCGGCACCGCGCAGTTGCTGGGTCTGCTGCACCAACTGGTGGCCGCCGA
TCAGCTGGATATCGCCGTGGAGCGGCAGGTCCGTCTGCTGCTGGCTGCAGCGCCGCTGGCCACCAGCAGCGCCAAGGCGC
TGGTGCGCTCGGTGCTGCCACCTGCCGACCGCGATGCCATCGATGCCGCCAATGCGGCGTTGATTGCCCGTCTGCGCGTG
TCGCCCGAAGGCCAGGAAGGCCTGGGGGCGTTCCTCGACAAGCGCAGCGCCGCGTGGGTGCCGGAGAGCATGGCGTGA

Upstream 100 bases:

>100_bases
TGTTAGGAATGGTTCTCAATTCTAGCATCGACGCACCGCGTCATGGCTCCGGCGACCCGGCCCGCTATCATGGTTCGCAT
CCCATTCATGGACGTACGCA

Downstream 100 bases:

>100_bases
TCGACCATATCGGCCTGGCCTGCAGCGATCTGCAAACCAGCACCGCGTTCTACCAGGCCGCGCTCGCGCCCCTGGGCATC
GCGCTGCTGGTGGAACTGAC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRIVVLTGDGAAFSAGADLNWMRGMASANEAE
NAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVACCDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQAR
RWFATAEIFDAGTAQLLGLLHQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPPADRDAIDAANAALIARLRV
SPEGQEGLGAFLDKRSAAWVPESMA

Sequences:

>Translated_265_residues
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRIVVLTGDGAAFSAGADLNWMRGMASANEAE
NAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVACCDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQAR
RWFATAEIFDAGTAQLLGLLHQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPPADRDAIDAANAALIARLRV
SPEGQEGLGAFLDKRSAAWVPESMA
>Mature_265_residues
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRIVVLTGDGAAFSAGADLNWMRGMASANEAE
NAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVACCDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQAR
RWFATAEIFDAGTAQLLGLLHQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPPADRDAIDAANAALIARLRV
SPEGQEGLGAFLDKRSAAWVPESMA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=26.1044176706827, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI194097323, Length=269, Percent_Identity=26.7657992565056, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI45643119, Length=256, Percent_Identity=26.171875, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI260275230, Length=256, Percent_Identity=26.171875, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=26.171875, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI68989263, Length=184, Percent_Identity=30.9782608695652, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI157694516, Length=192, Percent_Identity=23.9583333333333, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI213417737, Length=192, Percent_Identity=23.9583333333333, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=33.201581027668, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=30.1587301587302, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=27.1375464684015, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87082183, Length=252, Percent_Identity=25.3968253968254, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=31.1023622047244, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790281, Length=170, Percent_Identity=31.7647058823529, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=206, Percent_Identity=29.6116504854369, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=29.4573643410853, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=25.7383966244726, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=25.78125, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=27.9850746268657, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=25.390625, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129971, Length=253, Percent_Identity=28.8537549407115, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653477, Length=253, Percent_Identity=28.8537549407115, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=25.5506607929515, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27573; Mature: 27573

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRIVVLTGDGAA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
FSAGADLNWMRGMASANEAENAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVAC
EECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCCCCHHHHHH
CDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQARRWFATAEIFDAGTAQLLGLL
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPPADRDAIDAANAALIARLRV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEC
SPEGQEGLGAFLDKRSAAWVPESMA
CCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRIVVLTGDGAA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
FSAGADLNWMRGMASANEAENAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVAC
EECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCCCCHHHHHH
CDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQARRWFATAEIFDAGTAQLLGLL
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPPADRDAIDAANAALIARLRV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEC
SPEGQEGLGAFLDKRSAAWVPESMA
CCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA