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The map label for this gene is hadH2 [H]

Identifier: 21242594

GI number: 21242594

Start: 2146382

End: 2147152

Strand: Reverse

Name: hadH2 [H]

Synonym: XAC1850

Alternate gene names: 21242594

Gene position: 2147152-2146382 (Counterclockwise)

Preceding gene: 21242595

Following gene: 21242593

Centisome position: 41.49

GC content: 67.32

Gene sequence:

>771_bases
ATGCAGCCATCTTCTGTCCTTGCCATCGTGACCGGCGGCGTCTCCGGCCTCGGCCTGGCTGTCGCACAGCGTCTCGTGGC
CGACGGCGGCAAGGTCGCGCTGTTCGATGTCAACGCCGACAAGGCCGAGGCGGCGCTGGCGCTGCTCGGTGCCGGCAACG
CGCATTACCTCGCCACCGATGTCACCGATGAGGCCCAGGTCGCGGCCAATGTGGCGCAAGCCGCGCAGGCGCTGGGCGGA
TTGAATCTGGTGGTCAATTGCGCTGGCATCCTCGGCGCCGGCCGCGTATTGGGCAAGGAAGCGCCAATGCCGTTGGCCAC
CTTCCGCAACACCGTGCTGGTGAATCTGGTCGGCAGCTTCAATGTGGCCAAGGCCGCGGCCGATGTCATGCAACACAACA
CCACCGGCGATGACGGCGAGCGCGGCGTGATCGTCAATACCGCCTCGGTCGCAGCATTCGAGGGACAGATCGGCCAGGCA
GCATATGCGGCGAGCAAAGGCGGCGTGGTCGGCATGACCTTGCCGATGGCGCGCGAACTGGCCCGCTTCGGCATCCGCGT
GATGACGATCGCGCCGGGCGTGTTCTGGACCCCGATGGTCGACGGCATGCCCGAGGCCGTGCAGCAATCGCTGGCCGCCT
CCATCCCGTTCCCCTCGCGCCTCGGCCAGCCCGACGAATACGCCGACACCGTCGCCTTCATCCTGCGCAACCGCTACCTC
AATGGCGAAGTGATCCGCCTGGACGGCGGCGTGCGGTTGGCGCCCAAGTAG

Upstream 100 bases:

>100_bases
CCGCGATCACGCAGGGGCTTGGGCTAGAATGGCCGACCGCTCCGCCGATCGTTCGTTTGCCGCCACCACCGGCCATCAAC
GCGACTCAGGGAACCACCGC

Downstream 100 bases:

>100_bases
CGGGGATTGGGGAATCGGGAATGGGGAATCGTAACGGCGCCCTGCTCTCAGCACCCCGGACCCCGGTTTTTCGATTCCCC
ATTCCCTACTCCCGATTCCC

Product: 3-hydroxyacyl-CoA dehydrogenase type II

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MQPSSVLAIVTGGVSGLGLAVAQRLVADGGKVALFDVNADKAEAALALLGAGNAHYLATDVTDEAQVAANVAQAAQALGG
LNLVVNCAGILGAGRVLGKEAPMPLATFRNTVLVNLVGSFNVAKAAADVMQHNTTGDDGERGVIVNTASVAAFEGQIGQA
AYAASKGGVVGMTLPMARELARFGIRVMTIAPGVFWTPMVDGMPEAVQQSLAASIPFPSRLGQPDEYADTVAFILRNRYL
NGEVIRLDGGVRLAPK

Sequences:

>Translated_256_residues
MQPSSVLAIVTGGVSGLGLAVAQRLVADGGKVALFDVNADKAEAALALLGAGNAHYLATDVTDEAQVAANVAQAAQALGG
LNLVVNCAGILGAGRVLGKEAPMPLATFRNTVLVNLVGSFNVAKAAADVMQHNTTGDDGERGVIVNTASVAAFEGQIGQA
AYAASKGGVVGMTLPMARELARFGIRVMTIAPGVFWTPMVDGMPEAVQQSLAASIPFPSRLGQPDEYADTVAFILRNRYL
NGEVIRLDGGVRLAPK
>Mature_256_residues
MQPSSVLAIVTGGVSGLGLAVAQRLVADGGKVALFDVNADKAEAALALLGAGNAHYLATDVTDEAQVAANVAQAAQALGG
LNLVVNCAGILGAGRVLGKEAPMPLATFRNTVLVNLVGSFNVAKAAADVMQHNTTGDDGERGVIVNTASVAAFEGQIGQA
AYAASKGGVVGMTLPMARELARFGIRVMTIAPGVFWTPMVDGMPEAVQQSLAASIPFPSRLGQPDEYADTVAFILRNRYL
NGEVIRLDGGVRLAPK

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=252, Percent_Identity=51.984126984127, Blast_Score=240, Evalue=8e-64,
Organism=Homo sapiens, GI83715985, Length=252, Percent_Identity=48.4126984126984, Blast_Score=214, Evalue=4e-56,
Organism=Homo sapiens, GI15277342, Length=260, Percent_Identity=36.5384615384615, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI40254992, Length=244, Percent_Identity=34.8360655737705, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI31542939, Length=242, Percent_Identity=27.6859504132231, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI59889578, Length=245, Percent_Identity=26.530612244898, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI10190704, Length=259, Percent_Identity=28.957528957529, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=26.8, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5031737, Length=253, Percent_Identity=27.6679841897233, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4503817, Length=197, Percent_Identity=27.9187817258883, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=26.5151515151515, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787335, Length=255, Percent_Identity=30.9803921568627, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=30.3501945525292, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI2367175, Length=254, Percent_Identity=34.251968503937, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790717, Length=255, Percent_Identity=28.6274509803922, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=31.2, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789057, Length=265, Percent_Identity=28.3018867924528, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082160, Length=249, Percent_Identity=28.5140562248996, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=27.7992277992278, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786812, Length=262, Percent_Identity=30.5343511450382, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788459, Length=254, Percent_Identity=29.1338582677165, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789208, Length=250, Percent_Identity=28, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=251, Percent_Identity=45.0199203187251, Blast_Score=203, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI25147288, Length=254, Percent_Identity=32.2834645669291, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=33.8645418326693, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17561402, Length=260, Percent_Identity=32.3076923076923, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=252, Percent_Identity=26.5873015873016, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=271, Percent_Identity=28.0442804428044, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=201, Percent_Identity=30.8457711442786, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=26.8482490272374, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17563726, Length=268, Percent_Identity=25.3731343283582, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=266, Percent_Identity=25.5639097744361, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=202, Percent_Identity=30.1980198019802, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324126, Length=257, Percent_Identity=26.4591439688716, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737361, Length=251, Percent_Identity=50.199203187251, Blast_Score=231, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=37.3493975903614, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24665243, Length=205, Percent_Identity=30.7317073170732, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI221331218, Length=205, Percent_Identity=30.7317073170732, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355319, Length=251, Percent_Identity=29.8804780876494, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI23397609, Length=265, Percent_Identity=30.9433962264151, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24665247, Length=206, Percent_Identity=31.0679611650485, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221331216, Length=206, Percent_Identity=31.0679611650485, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571526, Length=252, Percent_Identity=30.5555555555556, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 26152; Mature: 26152

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSSVLAIVTGGVSGLGLAVAQRLVADGGKVALFDVNADKAEAALALLGAGNAHYLATD
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHEEEEECCCCEEEEEC
VTDEAQVAANVAQAAQALGGLNLVVNCAGILGAGRVLGKEAPMPLATFRNTVLVNLVGSF
CCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCHHHCCCCCCCHHHHHCEEEEEECCCC
NVAKAAADVMQHNTTGDDGERGVIVNTASVAAFEGQIGQAAYAASKGGVVGMTLPMAREL
HHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHCCCCEEEEEHHHHHHH
ARFGIRVMTIAPGVFWTPMVDGMPEAVQQSLAASIPFPSRLGQPDEYADTVAFILRNRYL
HHCCEEEEEECCCCEECCCCCCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCC
NGEVIRLDGGVRLAPK
CCEEEEECCCEEECCC
>Mature Secondary Structure
MQPSSVLAIVTGGVSGLGLAVAQRLVADGGKVALFDVNADKAEAALALLGAGNAHYLATD
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHEEEEECCCCEEEEEC
VTDEAQVAANVAQAAQALGGLNLVVNCAGILGAGRVLGKEAPMPLATFRNTVLVNLVGSF
CCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCHHHCCCCCCCHHHHHCEEEEEECCCC
NVAKAAADVMQHNTTGDDGERGVIVNTASVAAFEGQIGQAAYAASKGGVVGMTLPMAREL
HHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEECCCCCHHHHHHCCCCEEEEEHHHHHHH
ARFGIRVMTIAPGVFWTPMVDGMPEAVQQSLAASIPFPSRLGQPDEYADTVAFILRNRYL
HHCCEEEEEECCCCEECCCCCCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCC
NGEVIRLDGGVRLAPK
CCEEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]