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The map label for this gene is pcnB [H]

Identifier: 21242528

GI number: 21242528

Start: 2052441

End: 2053808

Strand: Direct

Name: pcnB [H]

Synonym: XAC1783

Alternate gene names: 21242528

Gene position: 2052441-2053808 (Clockwise)

Preceding gene: 21242527

Following gene: 21242529

Centisome position: 39.66

GC content: 64.25

Gene sequence:

>1368_bases
TTGGGCAATCCTGCAACACAAGACGGAAGCGCCATCATCGAACCCTCAGTTACATCTCCGTTCACGCTGCGCGTCATTCC
ACGCGACCAGCACACCATCTCGCGCAAGGACATCAGCCCCAACGCGTTACGCGTGTTGTACCGCCTGCGCGAATCGGGCT
TTGGTGCCTATCTGGTCGGCGGTGCGGTCCGCGACCTGCTGGTTGGCGGCCACCCGAAGGATTTCGACGTGGCCACCGAC
GCGACGCCGGAAGAGGTCAAGGCGCTGTTCCGCAACTGTCGCCTGATCGGCCGCCGTTTCCGTCTGGCACATGTGGTGTT
CGGTCGCGAAATCATCGAAGTGGCCACCTTCCGCGCCAATGTCGACGATGGCAGCGGCGACCGCGAACTCGATAACGGCC
GGCTGGTGCGCGACAACGTCTACGGCACCATCGAAGACGATGCGATCCGCCGCGACTTCACCTGCAATGCCTTGTATTAC
GCCATCGAGGATTTCTCGGTACGCGATTACTGCGGTGGCTTCGAAGACGTGCAGGCGCGCCTGATGAAGTTGATCGGCGA
CCCGCAGTTGCGTTACCAGGAAGACCCGGTGCGCATGCTGCGCGCGGTGCGTCTGGCTGCCAAGTTGAATTTCGACATCG
AAGCCGGAACCGCCGAACCCATTCCGCGTCTGGCCGGGCTGCTGTCCGAAGCTGCGCCTGCGCGGTTGTTCGAAGAAATT
CTCAAGCTGTTCCTGTCCGGGCATGGTGTGGCCAGCTTCGAAGGCCTGGAGCGTTACGGCCTGCTCGGTGCGCTGTTCCC
GGAAAGCGCGGCGGCATTGAAGTCCAACCGCAGTGGTGCGTTGCGCGCGATGGTGCTGGAAGGCCTGCGCAATACCGATG
CACGCGTGGCCAACGACGAGCCAGTGTCGCCGGCCTTCCTGTTTGCGTTGCTGCTGTGGCCTGCGTTCTGCCGCACCTTG
ATGGGGCTGCAGGCGCAAGGTGTGCAACCGGAGGATGCGCAGCGCCGCGCGGCCGATCGCGTCACCTTGCATCAGTTGGA
GCGCGTCGCGTTGCCGCGCCGTTTCTCTCTGCCGATGCAGGAAATCTGGTTGCTGCAGACGCGTTTCTCCTCGCGTCAGC
GCAAGCGGGTATTCCGCACCTTGTCGCATCCGCGCTTCCGCGCGGCATTCGATTTCCTGGTGTTGCGTCAGTTCGCGTCG
CCCGATCACGCCGCCGATGTGGAGTTCTGGCGCGAGGCGCAGAAGTCGTCCGGCCAGGAGCTGGTGGATGCGATCGAATC
GGCGCAGGCCGACCACGAGAGCGATGAGGGCGCACCGCGCAAGCGTCGCCGACGCCGCCGTCGGACCGGCGCACCGGCCG
GCGAGTAG

Upstream 100 bases:

>100_bases
CCCTGTTTTAGCCTTGAGAGGGCCACGTCCTAACCATTAGACGAAGGGAGCGCTTTTGTCACGTCTTGTGCGGCGCGCTA
GTATAGTGAGGTAATAGCCG

Downstream 100 bases:

>100_bases
GGCATGCAGACCGCCTTTGTCGGCCTGGGTGCCAACCTGGGCCCGGCCGAGGCCAGCGTGCGCGCGGCCATCGCCGCGCT
GGGCGCCGTGCCGCAAACCA

Product: polynucleotide adenyltransferase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MGNPATQDGSAIIEPSVTSPFTLRVIPRDQHTISRKDISPNALRVLYRLRESGFGAYLVGGAVRDLLVGGHPKDFDVATD
ATPEEVKALFRNCRLIGRRFRLAHVVFGREIIEVATFRANVDDGSGDRELDNGRLVRDNVYGTIEDDAIRRDFTCNALYY
AIEDFSVRDYCGGFEDVQARLMKLIGDPQLRYQEDPVRMLRAVRLAAKLNFDIEAGTAEPIPRLAGLLSEAAPARLFEEI
LKLFLSGHGVASFEGLERYGLLGALFPESAAALKSNRSGALRAMVLEGLRNTDARVANDEPVSPAFLFALLLWPAFCRTL
MGLQAQGVQPEDAQRRAADRVTLHQLERVALPRRFSLPMQEIWLLQTRFSSRQRKRVFRTLSHPRFRAAFDFLVLRQFAS
PDHAADVEFWREAQKSSGQELVDAIESAQADHESDEGAPRKRRRRRRRTGAPAGE

Sequences:

>Translated_455_residues
MGNPATQDGSAIIEPSVTSPFTLRVIPRDQHTISRKDISPNALRVLYRLRESGFGAYLVGGAVRDLLVGGHPKDFDVATD
ATPEEVKALFRNCRLIGRRFRLAHVVFGREIIEVATFRANVDDGSGDRELDNGRLVRDNVYGTIEDDAIRRDFTCNALYY
AIEDFSVRDYCGGFEDVQARLMKLIGDPQLRYQEDPVRMLRAVRLAAKLNFDIEAGTAEPIPRLAGLLSEAAPARLFEEI
LKLFLSGHGVASFEGLERYGLLGALFPESAAALKSNRSGALRAMVLEGLRNTDARVANDEPVSPAFLFALLLWPAFCRTL
MGLQAQGVQPEDAQRRAADRVTLHQLERVALPRRFSLPMQEIWLLQTRFSSRQRKRVFRTLSHPRFRAAFDFLVLRQFAS
PDHAADVEFWREAQKSSGQELVDAIESAQADHESDEGAPRKRRRRRRRTGAPAGE
>Mature_454_residues
GNPATQDGSAIIEPSVTSPFTLRVIPRDQHTISRKDISPNALRVLYRLRESGFGAYLVGGAVRDLLVGGHPKDFDVATDA
TPEEVKALFRNCRLIGRRFRLAHVVFGREIIEVATFRANVDDGSGDRELDNGRLVRDNVYGTIEDDAIRRDFTCNALYYA
IEDFSVRDYCGGFEDVQARLMKLIGDPQLRYQEDPVRMLRAVRLAAKLNFDIEAGTAEPIPRLAGLLSEAAPARLFEEIL
KLFLSGHGVASFEGLERYGLLGALFPESAAALKSNRSGALRAMVLEGLRNTDARVANDEPVSPAFLFALLLWPAFCRTLM
GLQAQGVQPEDAQRRAADRVTLHQLERVALPRRFSLPMQEIWLLQTRFSSRQRKRVFRTLSHPRFRAAFDFLVLRQFASP
DHAADVEFWREAQKSSGQELVDAIESAQADHESDEGAPRKRRRRRRRTGAPAGE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=199, Percent_Identity=31.1557788944724, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87081691, Length=417, Percent_Identity=50.8393285371703, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1789436, Length=218, Percent_Identity=33.4862385321101, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=258, Percent_Identity=26.3565891472868, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281360112, Length=214, Percent_Identity=29.4392523364486, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357337, Length=214, Percent_Identity=29.4392523364486, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 51029; Mature: 50897

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNPATQDGSAIIEPSVTSPFTLRVIPRDQHTISRKDISPNALRVLYRLRESGFGAYLVG
CCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHH
GAVRDLLVGGHPKDFDVATDATPEEVKALFRNCRLIGRRFRLAHVVFGREIIEVATFRAN
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDDGSGDRELDNGRLVRDNVYGTIEDDAIRRDFTCNALYYAIEDFSVRDYCGGFEDVQAR
CCCCCCCCCCCCCEEEECCCCEEECHHHHHCCCCCCHHEEHHHCCCHHHHHCCHHHHHHH
LMKLIGDPQLRYQEDPVRMLRAVRLAAKLNFDIEAGTAEPIPRLAGLLSEAAPARLFEEI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHH
LKLFLSGHGVASFEGLERYGLLGALFPESAAALKSNRSGALRAMVLEGLRNTDARVANDE
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
PVSPAFLFALLLWPAFCRTLMGLQAQGVQPEDAQRRAADRVTLHQLERVALPRRFSLPMQ
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EIWLLQTRFSSRQRKRVFRTLSHPRFRAAFDFLVLRQFASPDHAADVEFWREAQKSSGQE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHH
LVDAIESAQADHESDEGAPRKRRRRRRRTGAPAGE
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GNPATQDGSAIIEPSVTSPFTLRVIPRDQHTISRKDISPNALRVLYRLRESGFGAYLVG
CCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHH
GAVRDLLVGGHPKDFDVATDATPEEVKALFRNCRLIGRRFRLAHVVFGREIIEVATFRAN
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDDGSGDRELDNGRLVRDNVYGTIEDDAIRRDFTCNALYYAIEDFSVRDYCGGFEDVQAR
CCCCCCCCCCCCCEEEECCCCEEECHHHHHCCCCCCHHEEHHHCCCHHHHHCCHHHHHHH
LMKLIGDPQLRYQEDPVRMLRAVRLAAKLNFDIEAGTAEPIPRLAGLLSEAAPARLFEEI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHH
LKLFLSGHGVASFEGLERYGLLGALFPESAAALKSNRSGALRAMVLEGLRNTDARVANDE
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
PVSPAFLFALLLWPAFCRTLMGLQAQGVQPEDAQRRAADRVTLHQLERVALPRRFSLPMQ
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EIWLLQTRFSSRQRKRVFRTLSHPRFRAAFDFLVLRQFASPDHAADVEFWREAQKSSGQE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHH
LVDAIESAQADHESDEGAPRKRRRRRRRTGAPAGE
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]