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The map label for this gene is ispD

Identifier: 21242470

GI number: 21242470

Start: 1982436

End: 1983236

Strand: Direct

Name: ispD

Synonym: XAC1721

Alternate gene names: 21242470

Gene position: 1982436-1983236 (Clockwise)

Preceding gene: 21242469

Following gene: 21242471

Centisome position: 38.3

GC content: 68.04

Gene sequence:

>801_bases
ATGACCGGGTCCATCTGGGCAATCGTGCCGGCCGCCGGGCGCGGCACCCGTTTTGGCGGGCCGCTTCCCAAACAATATTT
GCAGGCCGGCGGTCAGCCACTGATGGCGTACACGTTGGCGGCGCTGGCCGCGCATCCGGCGCTGGCCGGCATCATGGTGG
CAATCGCCCCCGGCGATGCCGATTGGCCCGGCTGGACCGCGGTGCAGTCCAAGCCCGTGCTGACCTGTCTGGGGGGGGCG
ACCCGCGCAGCCTCGGTGCTGTCTGGTTTGCTGGCCTTGCCCGAGAGCGTGCGTGCCGACGATTTCGTGCTGGTGCACGA
TGCCGCACGGCCGAATCTGGCGCTGGCCGATCTGGATCGCCTGCTGGAAATCGGTCGCGGCGATCCGGTCGGCGCAATCC
TGGCTGCACCGGTGCGCGACACGCTCAAGCGTGCCGGCGACGATGGCGGTATCGACGGCACCGAACCGCGCGAACGCCTG
TGGCGCGCACTCACGCCGCAGTTGTTTCGTCGCCATCAACTGATCCGTGGACTCACCGAGGCCTCGGCGGCCGGTGTCGA
GGTCACCGATGAAGCGATGGCGATGGAGCGGATTGGGCTACGCCCATTGCTGGTGGAAGGCGCCGAAGACAACTTCAAGG
TGACGACCCCTGCGGATCTGGCGCGCTTCGAGTTTGAACTGGCCAACCGGGACTGTGGACCCGGGACCCGGGACCCGGAA
AGCGCGCATCCCCAGTCGTCCGTTTCGGCCTCTGCTTTCTCCGGTCCCGGGTCCCGGGCCCCCGGTCCCGAAGAGATCTA
A

Upstream 100 bases:

>100_bases
ACCGCGTGGTCGAGGATGCGCCGCTGCCGGCACCTGCATCGGCTGAGGCGTCGGCCCCCGCACAGCAAGCGCCTGAGCCT
GTCGATCCGGTGGATCACCC

Downstream 100 bases:

>100_bases
TCCATGTCCTTCAATTTCAGAATCGGCCAAGGCTACGACGTGCATGCGTTCGGCCCTGGCGACCATGTGATGCTTGGCGG
CGTGCGCGTGGCGCACAGCC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTGSIWAIVPAAGRGTRFGGPLPKQYLQAGGQPLMAYTLAALAAHPALAGIMVAIAPGDADWPGWTAVQSKPVLTCLGGA
TRAASVLSGLLALPESVRADDFVLVHDAARPNLALADLDRLLEIGRGDPVGAILAAPVRDTLKRAGDDGGIDGTEPRERL
WRALTPQLFRRHQLIRGLTEASAAGVEVTDEAMAMERIGLRPLLVEGAEDNFKVTTPADLARFEFELANRDCGPGTRDPE
SAHPQSSVSASAFSGPGSRAPGPEEI

Sequences:

>Translated_266_residues
MTGSIWAIVPAAGRGTRFGGPLPKQYLQAGGQPLMAYTLAALAAHPALAGIMVAIAPGDADWPGWTAVQSKPVLTCLGGA
TRAASVLSGLLALPESVRADDFVLVHDAARPNLALADLDRLLEIGRGDPVGAILAAPVRDTLKRAGDDGGIDGTEPRERL
WRALTPQLFRRHQLIRGLTEASAAGVEVTDEAMAMERIGLRPLLVEGAEDNFKVTTPADLARFEFELANRDCGPGTRDPE
SAHPQSSVSASAFSGPGSRAPGPEEI
>Mature_265_residues
TGSIWAIVPAAGRGTRFGGPLPKQYLQAGGQPLMAYTLAALAAHPALAGIMVAIAPGDADWPGWTAVQSKPVLTCLGGAT
RAASVLSGLLALPESVRADDFVLVHDAARPNLALADLDRLLEIGRGDPVGAILAAPVRDTLKRAGDDGGIDGTEPRERLW
RALTPQLFRRHQLIRGLTEASAAGVEVTDEAMAMERIGLRPLLVEGAEDNFKVTTPADLARFEFELANRDCGPGTRDPES
AHPQSSVSASAFSGPGSRAPGPEEI

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=237, Percent_Identity=28.6919831223629, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789104, Length=224, Percent_Identity=46.4285714285714, Blast_Score=171, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_XANAC (Q8PLR8)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642052.1
- ProteinModelPortal:   Q8PLR8
- SMR:   Q8PLR8
- GeneID:   1155792
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1721
- NMPDR:   fig|190486.1.peg.1696
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- ProtClustDB:   PRK00155
- BioCyc:   XAXO190486:XAC1721-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 27793; Mature: 27662

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSIWAIVPAAGRGTRFGGPLPKQYLQAGGQPLMAYTLAALAAHPALAGIMVAIAPGDA
CCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCC
DWPGWTAVQSKPVLTCLGGATRAASVLSGLLALPESVRADDFVLVHDAARPNLALADLDR
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCHHCCCCCEEEEECCCCCCCHHHHHHH
LLEIGRGDPVGAILAAPVRDTLKRAGDDGGIDGTEPRERLWRALTPQLFRRHQLIRGLTE
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
ASAAGVEVTDEAMAMERIGLRPLLVEGAEDNFKVTTPADLARFEFELANRDCGPGTRDPE
HHCCCCEEHHHHHHHHHHCCCEEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCC
SAHPQSSVSASAFSGPGSRAPGPEEI
CCCCCCCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGSIWAIVPAAGRGTRFGGPLPKQYLQAGGQPLMAYTLAALAAHPALAGIMVAIAPGDA
CCCEEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCC
DWPGWTAVQSKPVLTCLGGATRAASVLSGLLALPESVRADDFVLVHDAARPNLALADLDR
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCHHCCCCCEEEEECCCCCCCHHHHHHH
LLEIGRGDPVGAILAAPVRDTLKRAGDDGGIDGTEPRERLWRALTPQLFRRHQLIRGLTE
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
ASAAGVEVTDEAMAMERIGLRPLLVEGAEDNFKVTTPADLARFEFELANRDCGPGTRDPE
HHCCCCEEHHHHHHHHHHCCCEEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCC
SAHPQSSVSASAFSGPGSRAPGPEEI
CCCCCCCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217