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The map label for this gene is ispF

Identifier: 21242471

GI number: 21242471

Start: 1983240

End: 1983740

Strand: Direct

Name: ispF

Synonym: XAC1722

Alternate gene names: 21242471

Gene position: 1983240-1983740 (Clockwise)

Preceding gene: 21242470

Following gene: 21242472

Centisome position: 38.32

GC content: 65.07

Gene sequence:

>501_bases
ATGTCCTTCAATTTCAGAATCGGCCAAGGCTACGACGTGCATGCGTTCGGCCCTGGCGACCATGTGATGCTTGGCGGCGT
GCGCGTGGCGCACAGCCATGGCGTGCTCGCCCATAGCGATGGCGATGTGGTGTTGCACGCCCTGTGCGATGCGATGCTGG
GCGCGCTGGCGCTGGGCGATATCGGCCGGCATTTCCCGCCTTCCGATGCGCGCTGGAAAGATGCCGATAGCGCGCAGTTT
CTGCAGCATTGCGATGGCTTGCTGCGCGAGCGCGGCTGGCGCGTCGGCAATGCCGACATCACCGTGATCTGCGAGCGCCC
CAAGGTCGGTCCGCATGCGGTGGCCATGCGCGAGCGCATCGCCGACCTGTTGGCGATTGAACTCGATGCGGTCAGCGTCA
AGGCCACCACCAGCGAACAATTGGGCTTCACCGGCCGCGGCGAAGGCATCGCTGCACAGGCCGCGGTGTTGCTGGGCAGG
ATCGCTGCGCCTCATCAATAG

Upstream 100 bases:

>100_bases
GACCCGGGACCCGGAAAGCGCGCATCCCCAGTCGTCCGTTTCGGCCTCTGCTTTCTCCGGTCCCGGGTCCCGGGCCCCCG
GTCCCGAAGAGATCTAATCC

Downstream 100 bases:

>100_bases
CGACACGTGCAGCGCTTGGCGCCGGAGCGGCGCCGATGCTGCCCGATGCAATCACCGCGCCGAACCACCCGGCGCACAGC
GCGCCACCTCCGGCGCGAAG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSFNFRIGQGYDVHAFGPGDHVMLGGVRVAHSHGVLAHSDGDVVLHALCDAMLGALALGDIGRHFPPSDARWKDADSAQF
LQHCDGLLRERGWRVGNADITVICERPKVGPHAVAMRERIADLLAIELDAVSVKATTSEQLGFTGRGEGIAAQAAVLLGR
IAAPHQ

Sequences:

>Translated_166_residues
MSFNFRIGQGYDVHAFGPGDHVMLGGVRVAHSHGVLAHSDGDVVLHALCDAMLGALALGDIGRHFPPSDARWKDADSAQF
LQHCDGLLRERGWRVGNADITVICERPKVGPHAVAMRERIADLLAIELDAVSVKATTSEQLGFTGRGEGIAAQAAVLLGR
IAAPHQ
>Mature_165_residues
SFNFRIGQGYDVHAFGPGDHVMLGGVRVAHSHGVLAHSDGDVVLHALCDAMLGALALGDIGRHFPPSDARWKDADSAQFL
QHCDGLLRERGWRVGNADITVICERPKVGPHAVAMRERIADLLAIELDAVSVKATTSEQLGFTGRGEGIAAQAAVLLGRI
AAPHQ

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=53.8961038961039, Blast_Score=155, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_XANAC (Q8PLR7)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642053.1
- ProteinModelPortal:   Q8PLR7
- SMR:   Q8PLR7
- GeneID:   1155793
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1722
- NMPDR:   fig|190486.1.peg.1697
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   XAXO190486:XAC1722-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17645; Mature: 17514

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNFRIGQGYDVHAFGPGDHVMLGGVRVAHSHGVLAHSDGDVVLHALCDAMLGALALGD
CCCEEECCCCEEEEEECCCCEEEECCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHH
IGRHFPPSDARWKDADSAQFLQHCDGLLRERGWRVGNADITVICERPKVGPHAVAMRERI
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHH
ADLLAIELDAVSVKATTSEQLGFTGRGEGIAAQAAVLLGRIAAPHQ
HHHHHHEECCEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SFNFRIGQGYDVHAFGPGDHVMLGGVRVAHSHGVLAHSDGDVVLHALCDAMLGALALGD
CCEEECCCCEEEEEECCCCEEEECCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHH
IGRHFPPSDARWKDADSAQFLQHCDGLLRERGWRVGNADITVICERPKVGPHAVAMRERI
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHH
ADLLAIELDAVSVKATTSEQLGFTGRGEGIAAQAAVLLGRIAAPHQ
HHHHHHEECCEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217