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The map label for this gene is lysS [H]

Identifier: 21242378

GI number: 21242378

Start: 1875628

End: 1876590

Strand: Direct

Name: lysS [H]

Synonym: XAC1628

Alternate gene names: 21242378

Gene position: 1875628-1876590 (Clockwise)

Preceding gene: 21242377

Following gene: 21242379

Centisome position: 36.24

GC content: 64.69

Gene sequence:

>963_bases
ATGAACACGGCGCAGGTCGATCTGGCCGCGCTGCAGTTGCGGGCGCGGCTCAACGCCGCGATCCGCGACTTTTTCGCCGA
ACGCGGCGTGCTAGAGGTGGAAACGCCGGTGCTTTCGGAGGCCGGCAATACAGAACCGAATATCGAGAGTTTCAGTACCC
GCTTCACCGGCCATGTCGATGCCGGCGCGCCGCTGCGCTGGTTGCGCACCTCGCCGGAATATCCGCTCAAACGCCTGCTT
GCCGCCGGCGTGGGCGATTGCTACGAACTGGGGCGGGTGTTTCGCAATGGCGAAGCCGGTGGCCGTCACAATCCCGAGTT
CAGCATGCTCGAATGGTATCGGGTGGGTTGGAACGATCGCAGTCTGGCGCAGGAGACCATCGAACTGGTGCGTCGCGCGC
TGGCGCTGGTCGGGCGCCAGGCGAGGGTGCAGGTGCTGACGTATCGCGCGCTCTTCGTGCAGACGTTGGGCATCGACCCA
TTGTGCGATCCGATCGAAACGCTACACGCCGCCTTGCATGACGTGGCGATCGATCCTGTGGGTCTGACCCGCGACGATTG
GCTGGACCTGCTGATGACCCATCGCATCCAGCCGGGCTTTGCCAGCGATACCCTGACGGTGGTGCACGATTGGCCGGCCA
GTCAATGTGCGTTGGCGCGGATCCGCCACGACGACCCACCGGTGGCCGAGCGCTTCGAGCTGTATCTGGGCAGTTTCGAG
GTGGCCAATGGCTATCACGAATTGAACGATGCGCAGGAGCAGCGTGCGCGCTTCGTGCGCGACAATGCCGTGCGTGCAGC
ACGTGGATTGCCGCAACTGCCACTGGACGAACGCCTGCTGGCGGTGTTGTCGCAGTTGCCCGACTGCGCCGGTGTGGCGG
TGGGCGTGGATCGCCTGTTGATGGCCTTGCGCGGCACTACGCAGATTGCCGACGTGCTGGCGTTCGACTTCGCCCGCGCC
TGA

Upstream 100 bases:

>100_bases
AAGAAGCCGGTTCCAAGCTCGACAAGGCGCAATCGCTGGGCGTGGAGATCTGGGACGAAGCGCGTTTGCTGGCCTTCCTG
GGCGACCACGGCCAACAGCC

Downstream 100 bases:

>100_bases
GTCATCTGGCTGTCATCGGCGCCGTATTGCTATCTTCACATCGATGATGCTCTCATCTTCGCGTCCACGGGGGCGCGACG
AAGATCGTTCGGGGTGAGGA

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MNTAQVDLAALQLRARLNAAIRDFFAERGVLEVETPVLSEAGNTEPNIESFSTRFTGHVDAGAPLRWLRTSPEYPLKRLL
AAGVGDCYELGRVFRNGEAGGRHNPEFSMLEWYRVGWNDRSLAQETIELVRRALALVGRQARVQVLTYRALFVQTLGIDP
LCDPIETLHAALHDVAIDPVGLTRDDWLDLLMTHRIQPGFASDTLTVVHDWPASQCALARIRHDDPPVAERFELYLGSFE
VANGYHELNDAQEQRARFVRDNAVRAARGLPQLPLDERLLAVLSQLPDCAGVAVGVDRLLMALRGTTQIADVLAFDFARA

Sequences:

>Translated_320_residues
MNTAQVDLAALQLRARLNAAIRDFFAERGVLEVETPVLSEAGNTEPNIESFSTRFTGHVDAGAPLRWLRTSPEYPLKRLL
AAGVGDCYELGRVFRNGEAGGRHNPEFSMLEWYRVGWNDRSLAQETIELVRRALALVGRQARVQVLTYRALFVQTLGIDP
LCDPIETLHAALHDVAIDPVGLTRDDWLDLLMTHRIQPGFASDTLTVVHDWPASQCALARIRHDDPPVAERFELYLGSFE
VANGYHELNDAQEQRARFVRDNAVRAARGLPQLPLDERLLAVLSQLPDCAGVAVGVDRLLMALRGTTQIADVLAFDFARA
>Mature_320_residues
MNTAQVDLAALQLRARLNAAIRDFFAERGVLEVETPVLSEAGNTEPNIESFSTRFTGHVDAGAPLRWLRTSPEYPLKRLL
AAGVGDCYELGRVFRNGEAGGRHNPEFSMLEWYRVGWNDRSLAQETIELVRRALALVGRQARVQVLTYRALFVQTLGIDP
LCDPIETLHAALHDVAIDPVGLTRDDWLDLLMTHRIQPGFASDTLTVVHDWPASQCALARIRHDDPPVAERFELYLGSFE
VANGYHELNDAQEQRARFVRDNAVRAARGLPQLPLDERLLAVLSQLPDCAGVAVGVDRLLMALRGTTQIADVLAFDFARA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87082379, Length=311, Percent_Identity=45.6591639871383, Blast_Score=254, Evalue=5e-69,
Organism=Escherichia coli, GI1790571, Length=331, Percent_Identity=30.5135951661631, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789256, Length=331, Percent_Identity=29.3051359516616, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320242, Length=339, Percent_Identity=29.4985250737463, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324256, Length=351, Percent_Identity=27.6353276353276, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 35584; Mature: 35584

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAQVDLAALQLRARLNAAIRDFFAERGVLEVETPVLSEAGNTEPNIESFSTRFTGHVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCCCHHHHHHHHCCCCC
AGAPLRWLRTSPEYPLKRLLAAGVGDCYELGRVFRNGEAGGRHNPEFSMLEWYRVGWNDR
CCCCHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCH
SLAQETIELVRRALALVGRQARVQVLTYRALFVQTLGIDPLCDPIETLHAALHDVAIDPV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
GLTRDDWLDLLMTHRIQPGFASDTLTVVHDWPASQCALARIRHDDPPVAERFELYLGSFE
CCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHCCHH
VANGYHELNDAQEQRARFVRDNAVRAARGLPQLPLDERLLAVLSQLPDCAGVAVGVDRLL
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
MALRGTTQIADVLAFDFARA
HHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTAQVDLAALQLRARLNAAIRDFFAERGVLEVETPVLSEAGNTEPNIESFSTRFTGHVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCCCHHHHHHHHCCCCC
AGAPLRWLRTSPEYPLKRLLAAGVGDCYELGRVFRNGEAGGRHNPEFSMLEWYRVGWNDR
CCCCHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCH
SLAQETIELVRRALALVGRQARVQVLTYRALFVQTLGIDPLCDPIETLHAALHDVAIDPV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
GLTRDDWLDLLMTHRIQPGFASDTLTVVHDWPASQCALARIRHDDPPVAERFELYLGSFE
CCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHCCHH
VANGYHELNDAQEQRARFVRDNAVRAARGLPQLPLDERLLAVLSQLPDCAGVAVGVDRLL
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
MALRGTTQIADVLAFDFARA
HHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA