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The map label for this gene is ligA

Identifier: 21242377

GI number: 21242377

Start: 1873130

End: 1875631

Strand: Direct

Name: ligA

Synonym: XAC1627

Alternate gene names: 21242377

Gene position: 1873130-1875631 (Clockwise)

Preceding gene: 21242375

Following gene: 21242378

Centisome position: 36.19

GC content: 66.51

Gene sequence:

>2502_bases
ATGACTGCCAGCCCGGATCCCGCTCAGCGCATCGATGCCCTGCGCCGTCGAATCGAAGACGCCAACTACCGGTATCACGT
ACTCGACGAGCCGCAGATGGCCGATGCCGATTACGACAAGCTGATGCGCGAGCTTGAGGCCTTGGAGCGCGCGCACCCGG
AATTGGCCAGCGCCGATTCGCCAACCCAGCGCGTCGGCCATCTGGCCGCCTCGCGGTTTGCCGAAGTGCGGCATGCCATG
CCGATGCTGTCGTTGGGCAATGCCTTCAGCGATGAGGAAGTGACCGAGTTCGTGCGGCGCATCAGCGAGCGGCTGGAGGT
CAGACAACCGCTGTTTTCGGCCGAACCCAAACTCGATGGCCTGGCGATCAGCCTGCGCTACGAAAATGGCGAGTTCGTGC
AGGGCGCCACGCGCGGCGATGGCGCCACCGGCGAGGATGTCAGCGCCAACCTGCGCACCGTCAAAGCGATTCCGTTGCGT
CTGCGCGGCGAGGGTTGGCCGCGGGTGCTGGAAGTGCGCGGCGAGGTCTACATGCCACGTGCTGCGTTCGAAGCCTACAA
CGCGCAGATGCGTGCCCAGGGCGGCAAGATACTGGCCAACCCGCGCAATGGCGCGGCCGGCTCGCTGCGGCAGCTGGATG
CGCGCATCACCGCGCAGCGCCCGCTGAGCTTCTTCGCCTACGGCGTTGGCGAAGTGAGCGAAGGCGCATTGCCTCAGGCC
CATTCGGCGATCCTGGCGCAGCTGCGCGCGTGGGGTTTTCCGGTCAGCGCGCTGGTCGAGGTCGTGCAGGGCAGCGATGG
TCTGCTGGCGTATTACCAGCGCATCGGTGAGGCGCGCGATGGCCTGGCCTTCGATATCGATGGCGTGGTGTACAAGCTCG
ACGATCTGGCCGGGCAACGCGAAATGGGCTTCGTCTCGCGCGCGCCGCGTTGGGCGATCGCGCACAAGTTCCCCGCACAG
GAGCAGTCCACCACGGTGGAGGCGATCGAGATCCAGATCGGCCGCACCGGTGCGGCCACGCCGGTGGCGCGCCTGAAGCC
GGTGCATGTGGCCGGGGTGATCGTCACCAATGCCACGCTGCACAACGCCGACCAGATCGCGCGGCTGGATGTGCGCGTGG
GCGACACGGTGATCGTGCGCCGCGCCGGTGATGTCATCCCTGAAGTGGCCGCGGTGGTGGCCGACCAGCGCCCGCCCGGC
ACCCAGGCCTGGCAGATGCCCACGCAGTGCCCGGTGTGCGGTTCGGAGATCGTGCGCGAAGAAGGCCAGGCGGTCTGGCG
CTGTTCCGGCGAGTTGACCTGCCCGGCGCAGCGCAAGGAGGCGTTTCGGCATTTCGTCTCGCGGCGCGCGATGGATGTGG
ACGGCCTGGGCGAAAAATTCATCGAAGTGCTGGTCGACAGTGGCCTGGTGAAGGGCGTGGCCGACCTGTATCTGCTGAGC
GTGGACCAGCTGCTGCAATTGCGCCTGATCAGCACGGCCGACAGCCCGCACGCGTTCTTGCGCGAGGCGCGCGAGCATCT
GGCCAGCGGTGCATATGCGCAGCTGGAAGCCAGCGTGGTCGGGATCGGCGTGGACCTGGCCGGGGAGCGCGACGTGCCGC
AGACCTGGCAGGCCGATCTGCTCCGCGCAGGCTTGCCGAGCTTCGACTGGAACCGCAAGAAGATCGCCACCAAGTGGGCG
GAGAATCTGATCGAGGCGATCGAAATATCGCGCGATACCACGCTGGAGCGTTTTCTGTTTGCGCTGGGCATCGAGCATGT
GGGCGAAAGCACGGCCAAGGCCTTGTCGGCCTGGTTCGGCGATCTGGAATTGATCCGGCATCTGCCGTGGCCGCTGTTCA
AGCGCGTGCCGGATATCGGCGGCGAAGTGGCGCGCTCGCTGGGGCACTTCTTCGATCAGGCCGGCAATCAGAAGGCCATC
GATCATCTGCTGGCGCGCAAGGTGCGCATCGGCGACACCCATCCGCCTTCGCCGAAGCTGCGCGGCGAGCTGAGCTTGGC
CAATTTGCTGGAGGACCTGGAGATTCCCAAGGTGACGCCGATTCGCGCCGCCCAGATCGCGACTGCGTTTGGTTCCATCG
ATGCCCTGCGCAATGGCGGTCCCGAGCCTCTCGTCGAAGCCGGTGTACCGCAGTCAGTGGCCGAGTCGCTCGCGGCCTGG
CTGTTGGTGCCGGCCAACGATACGCTCGCGGTTAATGCCCAGAGAAAGCTTTCCGAGCTGCTGGCGATGCTGCCGGAGGC
CGGCGAGGAAAAGACCGGCCCGCTCGATGGCCAGACCGTGGTCATCACCGGGACGCTGGCTGCGCTGACGCGCGATGCGG
CCAAGCAGCGGCTGGAAGCGCTGGGCGCCAAGGTCGCCGGTAGCGTCTCCAAGAAGACCGCGTTTCTGGTGGCCGGCGAA
GAAGCCGGTTCCAAGCTCGACAAGGCGCAATCGCTGGGCGTGGAGATCTGGGACGAAGCGCGTTTGCTGGCCTTCCTGGG
CGACCACGGCCAACAGCCATGA

Upstream 100 bases:

>100_bases
TCATCCATCGCTCAGTCATCGCGAACGGCGCCCGCGCCGCTGACCTTGGCATCGGTGTGGCCCGTTAGAATGGGCGGCCC
AGAGCCAGCCCTGCGATCGT

Downstream 100 bases:

>100_bases
ACACGGCGCAGGTCGATCTGGCCGCGCTGCAGTTGCGGGCGCGGCTCAACGCCGCGATCCGCGACTTTTTCGCCGAACGC
GGCGTGCTAGAGGTGGAAAC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MTASPDPAQRIDALRRRIEDANYRYHVLDEPQMADADYDKLMRELEALERAHPELASADSPTQRVGHLAASRFAEVRHAM
PMLSLGNAFSDEEVTEFVRRISERLEVRQPLFSAEPKLDGLAISLRYENGEFVQGATRGDGATGEDVSANLRTVKAIPLR
LRGEGWPRVLEVRGEVYMPRAAFEAYNAQMRAQGGKILANPRNGAAGSLRQLDARITAQRPLSFFAYGVGEVSEGALPQA
HSAILAQLRAWGFPVSALVEVVQGSDGLLAYYQRIGEARDGLAFDIDGVVYKLDDLAGQREMGFVSRAPRWAIAHKFPAQ
EQSTTVEAIEIQIGRTGAATPVARLKPVHVAGVIVTNATLHNADQIARLDVRVGDTVIVRRAGDVIPEVAAVVADQRPPG
TQAWQMPTQCPVCGSEIVREEGQAVWRCSGELTCPAQRKEAFRHFVSRRAMDVDGLGEKFIEVLVDSGLVKGVADLYLLS
VDQLLQLRLISTADSPHAFLREAREHLASGAYAQLEASVVGIGVDLAGERDVPQTWQADLLRAGLPSFDWNRKKIATKWA
ENLIEAIEISRDTTLERFLFALGIEHVGESTAKALSAWFGDLELIRHLPWPLFKRVPDIGGEVARSLGHFFDQAGNQKAI
DHLLARKVRIGDTHPPSPKLRGELSLANLLEDLEIPKVTPIRAAQIATAFGSIDALRNGGPEPLVEAGVPQSVAESLAAW
LLVPANDTLAVNAQRKLSELLAMLPEAGEEKTGPLDGQTVVITGTLAALTRDAAKQRLEALGAKVAGSVSKKTAFLVAGE
EAGSKLDKAQSLGVEIWDEARLLAFLGDHGQQP

Sequences:

>Translated_833_residues
MTASPDPAQRIDALRRRIEDANYRYHVLDEPQMADADYDKLMRELEALERAHPELASADSPTQRVGHLAASRFAEVRHAM
PMLSLGNAFSDEEVTEFVRRISERLEVRQPLFSAEPKLDGLAISLRYENGEFVQGATRGDGATGEDVSANLRTVKAIPLR
LRGEGWPRVLEVRGEVYMPRAAFEAYNAQMRAQGGKILANPRNGAAGSLRQLDARITAQRPLSFFAYGVGEVSEGALPQA
HSAILAQLRAWGFPVSALVEVVQGSDGLLAYYQRIGEARDGLAFDIDGVVYKLDDLAGQREMGFVSRAPRWAIAHKFPAQ
EQSTTVEAIEIQIGRTGAATPVARLKPVHVAGVIVTNATLHNADQIARLDVRVGDTVIVRRAGDVIPEVAAVVADQRPPG
TQAWQMPTQCPVCGSEIVREEGQAVWRCSGELTCPAQRKEAFRHFVSRRAMDVDGLGEKFIEVLVDSGLVKGVADLYLLS
VDQLLQLRLISTADSPHAFLREAREHLASGAYAQLEASVVGIGVDLAGERDVPQTWQADLLRAGLPSFDWNRKKIATKWA
ENLIEAIEISRDTTLERFLFALGIEHVGESTAKALSAWFGDLELIRHLPWPLFKRVPDIGGEVARSLGHFFDQAGNQKAI
DHLLARKVRIGDTHPPSPKLRGELSLANLLEDLEIPKVTPIRAAQIATAFGSIDALRNGGPEPLVEAGVPQSVAESLAAW
LLVPANDTLAVNAQRKLSELLAMLPEAGEEKTGPLDGQTVVITGTLAALTRDAAKQRLEALGAKVAGSVSKKTAFLVAGE
EAGSKLDKAQSLGVEIWDEARLLAFLGDHGQQP
>Mature_832_residues
TASPDPAQRIDALRRRIEDANYRYHVLDEPQMADADYDKLMRELEALERAHPELASADSPTQRVGHLAASRFAEVRHAMP
MLSLGNAFSDEEVTEFVRRISERLEVRQPLFSAEPKLDGLAISLRYENGEFVQGATRGDGATGEDVSANLRTVKAIPLRL
RGEGWPRVLEVRGEVYMPRAAFEAYNAQMRAQGGKILANPRNGAAGSLRQLDARITAQRPLSFFAYGVGEVSEGALPQAH
SAILAQLRAWGFPVSALVEVVQGSDGLLAYYQRIGEARDGLAFDIDGVVYKLDDLAGQREMGFVSRAPRWAIAHKFPAQE
QSTTVEAIEIQIGRTGAATPVARLKPVHVAGVIVTNATLHNADQIARLDVRVGDTVIVRRAGDVIPEVAAVVADQRPPGT
QAWQMPTQCPVCGSEIVREEGQAVWRCSGELTCPAQRKEAFRHFVSRRAMDVDGLGEKFIEVLVDSGLVKGVADLYLLSV
DQLLQLRLISTADSPHAFLREAREHLASGAYAQLEASVVGIGVDLAGERDVPQTWQADLLRAGLPSFDWNRKKIATKWAE
NLIEAIEISRDTTLERFLFALGIEHVGESTAKALSAWFGDLELIRHLPWPLFKRVPDIGGEVARSLGHFFDQAGNQKAID
HLLARKVRIGDTHPPSPKLRGELSLANLLEDLEIPKVTPIRAAQIATAFGSIDALRNGGPEPLVEAGVPQSVAESLAAWL
LVPANDTLAVNAQRKLSELLAMLPEAGEEKTGPLDGQTVVITGTLAALTRDAAKQRLEALGAKVAGSVSKKTAFLVAGEE
AGSKLDKAQSLGVEIWDEARLLAFLGDHGQQP

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=73, Percent_Identity=50.6849315068493, Blast_Score=67, Evalue=9e-11,
Organism=Escherichia coli, GI1788750, Length=651, Percent_Identity=47.7726574500768, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI87082305, Length=399, Percent_Identity=24.812030075188, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_XANAC (Q8PM07)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641959.1
- HSSP:   O87703
- ProteinModelPortal:   Q8PM07
- GeneID:   1155698
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1627
- NMPDR:   fig|190486.1.peg.1603
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   XAXO190486:XAC1627-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 90485; Mature: 90354

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 117-117 BINDING 115-115 BINDING 138-138 BINDING 175-175 BINDING 292-292 BINDING 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPDPAQRIDALRRRIEDANYRYHVLDEPQMADADYDKLMRELEALERAHPELASADS
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PTQRVGHLAASRFAEVRHAMPMLSLGNAFSDEEVTEFVRRISERLEVRQPLFSAEPKLDG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCC
LAISLRYENGEFVQGATRGDGATGEDVSANLRTVKAIPLRLRGEGWPRVLEVRGEVYMPR
EEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEEECEEECCCCCCEEEEECCCEECCH
AAFEAYNAQMRAQGGKILANPRNGAAGSLRQLDARITAQRPLSFFAYGVGEVSEGALPQA
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHH
HSAILAQLRAWGFPVSALVEVVQGSDGLLAYYQRIGEARDGLAFDIDGVVYKLDDLAGQR
HHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCEEEEHHHCCCH
EMGFVSRAPRWAIAHKFPAQEQSTTVEAIEIQIGRTGAATPVARLKPVHVAGVIVTNATL
HCCHHHCCCCEEHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHCCCEEEEEEEEECCCC
HNADQIARLDVRVGDTVIVRRAGDVIPEVAAVVADQRPPGTQAWQMPTQCPVCGSEIVRE
CCCHHHEEEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
EGQAVWRCSGELTCPAQRKEAFRHFVSRRAMDVDGLGEKFIEVLVDSGLVKGVADLYLLS
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VDQLLQLRLISTADSPHAFLREAREHLASGAYAQLEASVVGIGVDLAGERDVPQTWQADL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHEEEEEEEEEEEEECCCCCCCHHHHHHH
LRAGLPSFDWNRKKIATKWAENLIEAIEISRDTTLERFLFALGIEHVGESTAKALSAWFG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
DLELIRHLPWPLFKRVPDIGGEVARSLGHFFDQAGNQKAIDHLLARKVRIGDTHPPSPKL
HHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
RGELSLANLLEDLEIPKVTPIRAAQIATAFGSIDALRNGGPEPLVEAGVPQSVAESLAAW
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHE
LLVPANDTLAVNAQRKLSELLAMLPEAGEEKTGPLDGQTVVITGTLAALTRDAAKQRLEA
EEECCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
LGAKVAGSVSKKTAFLVAGEEAGSKLDKAQSLGVEIWDEARLLAFLGDHGQQP
HHHHHHCCCCCCEEEEEECCHHCHHHHHHHHCCCEECCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
TASPDPAQRIDALRRRIEDANYRYHVLDEPQMADADYDKLMRELEALERAHPELASADS
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PTQRVGHLAASRFAEVRHAMPMLSLGNAFSDEEVTEFVRRISERLEVRQPLFSAEPKLDG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCC
LAISLRYENGEFVQGATRGDGATGEDVSANLRTVKAIPLRLRGEGWPRVLEVRGEVYMPR
EEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEEECEEECCCCCCEEEEECCCEECCH
AAFEAYNAQMRAQGGKILANPRNGAAGSLRQLDARITAQRPLSFFAYGVGEVSEGALPQA
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHH
HSAILAQLRAWGFPVSALVEVVQGSDGLLAYYQRIGEARDGLAFDIDGVVYKLDDLAGQR
HHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCEEEEHHHCCCH
EMGFVSRAPRWAIAHKFPAQEQSTTVEAIEIQIGRTGAATPVARLKPVHVAGVIVTNATL
HCCHHHCCCCEEHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHCCCEEEEEEEEECCCC
HNADQIARLDVRVGDTVIVRRAGDVIPEVAAVVADQRPPGTQAWQMPTQCPVCGSEIVRE
CCCHHHEEEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
EGQAVWRCSGELTCPAQRKEAFRHFVSRRAMDVDGLGEKFIEVLVDSGLVKGVADLYLLS
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VDQLLQLRLISTADSPHAFLREAREHLASGAYAQLEASVVGIGVDLAGERDVPQTWQADL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHEEEEEEEEEEEEECCCCCCCHHHHHHH
LRAGLPSFDWNRKKIATKWAENLIEAIEISRDTTLERFLFALGIEHVGESTAKALSAWFG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
DLELIRHLPWPLFKRVPDIGGEVARSLGHFFDQAGNQKAIDHLLARKVRIGDTHPPSPKL
HHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
RGELSLANLLEDLEIPKVTPIRAAQIATAFGSIDALRNGGPEPLVEAGVPQSVAESLAAW
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHE
LLVPANDTLAVNAQRKLSELLAMLPEAGEEKTGPLDGQTVVITGTLAALTRDAAKQRLEA
EEECCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
LGAKVAGSVSKKTAFLVAGEEAGSKLDKAQSLGVEIWDEARLLAFLGDHGQQP
HHHHHHCCCCCCEEEEEECCHHCHHHHHHHHCCCEECCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217