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The map label for this gene is holB [H]

Identifier: 21241887

GI number: 21241887

Start: 1282627

End: 1283586

Strand: Direct

Name: holB [H]

Synonym: XAC1132

Alternate gene names: 21241887

Gene position: 1282627-1283586 (Clockwise)

Preceding gene: 21241886

Following gene: 21241888

Centisome position: 24.78

GC content: 67.92

Gene sequence:

>960_bases
ATGACCGCCGCATTTTCGCCCTGGCAGCAGCGCGCCTTCGACCAGACCGTGGCTGCACTGGATGCGGGGCGCCTTGGACA
TGGGTTGCTGATCTGCGGCCCGGAAGGTCTGGGCAAGCGTGCCGTCGCCCTGGCCCTGGCCGAGCACGTCCTGGCCAGCT
CGCCAGACCCGGCACTGGCGCAGCGCACGCGCCAGCTGATTGCCGCCGGTACCCATCCGGACCTGCAACTGGTCTCGTTC
ATTCCCAATCGCACCGGCGACAAGCTGCGTAACGAGATCGTCATCGAGCAGGTGCGCGAGATTTCGCAGAAGCTCGCGCT
GACCCCGCAGTACGGCATTGCCCAGGTCGTGATCGTCGACCCGGCCGATGCGATCAATCGCTCGGCCTGCAATGCCTTGC
TCAAGACCCTGGAAGAGCCGTCGCCGGGGCGCTACCTGTGGTTGATCAGCGCGCAGCCAGCGCGTCTACCGGCGACCATC
CGCAGCCGCTGCCAGCGCCTGGAATTCAAGTTGCCGCCTGCGCACGAGGCGCTGGCCTGGTTGCTCTCGGAAGGTGTGAG
CGAGCGCGCCGCACAGGAAGCGCTGGACGCCGCGCGCGGCCACCCCGGTCTGGCCGCGCAGTGGTTGCGCGAAGATGGCC
TGGCCGTCCGGCGTGCGGTTGCGCAGGATCTGGAGCAGATCGCCAATGGACGCGCCGGCGCAGTCGATGTCGCGCAGCGT
TGGACCAACGATGGCCAGGCCGACCAGCGCCTGCGCCATGCCGCCGATCTGGCATTGGCACAGGCATCGGCCGGCTTGAC
CGATCCATCGCGGTTGCACAAGCTTGCGACCTGGTTCGACGCTGCCAACCGCACCCGCGATCTGCTGCGCACCACCGTCC
GCGCCGACCTGGCGGTGACGGAATTGTTGCTGTCCTGGCGCGAGGGAGAGCGCCAGCCACGTTCTAGGGGAACTCGATGA

Upstream 100 bases:

>100_bases
CGCACGCGTTCTCGGCCACGTTGGCCGAACATAACGCAGCGGTGGCGCGCTATCTGCAGCGCCGCCGGCTGCCGAGCACG
CCGCAGACGGAGCCTATGCC

Downstream 100 bases:

>100_bases
GTGCAATGAATGCACGCCAAGGCATTTTGTCGCTGGCGTTGAAAGACAAGCCAGCGCTCTACAGCGCCTACATGCCGTTC
GTGAAAGGCGGCGGCATCTT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTAAFSPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALAQRTRQLIAAGTHPDLQLVSF
IPNRTGDKLRNEIVIEQVREISQKLALTPQYGIAQVVIVDPADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATI
RSRCQRLEFKLPPAHEALAWLLSEGVSERAAQEALDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIANGRAGAVDVAQR
WTNDGQADQRLRHAADLALAQASAGLTDPSRLHKLATWFDAANRTRDLLRTTVRADLAVTELLLSWREGERQPRSRGTR

Sequences:

>Translated_319_residues
MTAAFSPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALAQRTRQLIAAGTHPDLQLVSF
IPNRTGDKLRNEIVIEQVREISQKLALTPQYGIAQVVIVDPADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATI
RSRCQRLEFKLPPAHEALAWLLSEGVSERAAQEALDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIANGRAGAVDVAQR
WTNDGQADQRLRHAADLALAQASAGLTDPSRLHKLATWFDAANRTRDLLRTTVRADLAVTELLLSWREGERQPRSRGTR
>Mature_318_residues
TAAFSPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALAQRTRQLIAAGTHPDLQLVSFI
PNRTGDKLRNEIVIEQVREISQKLALTPQYGIAQVVIVDPADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATIR
SRCQRLEFKLPPAHEALAWLLSEGVSERAAQEALDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIANGRAGAVDVAQRW
TNDGQADQRLRHAADLALAQASAGLTDPSRLHKLATWFDAANRTRDLLRTTVRADLAVTELLLSWREGERQPRSRGTR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=224, Percent_Identity=35.7142857142857, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786676, Length=167, Percent_Identity=29.3413173652695, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34757; Mature: 34626

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAFSPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHH
QRTRQLIAAGTHPDLQLVSFIPNRTGDKLRNEIVIEQVREISQKLALTPQYGIAQVVIVD
HHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATIRSRCQRLEFKLPPAHEALAW
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
LLSEGVSERAAQEALDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIANGRAGAVDVAQR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
WTNDGQADQRLRHAADLALAQASAGLTDPSRLHKLATWFDAANRTRDLLRTTVRADLAVT
HCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLLSWREGERQPRSRGTR
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAAFSPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALA
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHH
QRTRQLIAAGTHPDLQLVSFIPNRTGDKLRNEIVIEQVREISQKLALTPQYGIAQVVIVD
HHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATIRSRCQRLEFKLPPAHEALAW
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
LLSEGVSERAAQEALDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIANGRAGAVDVAQR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
WTNDGQADQRLRHAADLALAQASAGLTDPSRLHKLATWFDAANRTRDLLRTTVRADLAVT
HCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLLSWREGERQPRSRGTR
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]