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The map label for this gene is gloB

Identifier: 21241844

GI number: 21241844

Start: 1242424

End: 1243191

Strand: Reverse

Name: gloB

Synonym: XAC1087

Alternate gene names: 21241844

Gene position: 1243191-1242424 (Counterclockwise)

Preceding gene: 21241848

Following gene: 21241843

Centisome position: 24.02

GC content: 64.32

Gene sequence:

>768_bases
ATGCGACTGATCGCCCTGCCCGCATTCGACGACAACTACATCTGGGCGCTGGTCGCCGCAGATGGCCGCGCCATCATTGT
CGACCCCGGTCAAGCCGAGCCTGTCCTGGCTGCCGCGCAGCGCCAGGGCCTGGTGCCGAGCGCCGTCTTGCTGACCCATC
ACCACGGCGACCACATCGGCGGCGTTGCCGAACTCCAGCAGCGCTGGCCGGACCTGGCGCTGTTCGGCCCGGCCGACGAA
CGCATCCCGACCAACGCGCATCATGTGGGTCGCGGCGAGCGCTTGCGCCTGCTCGATGTCGAGTTCCAGGTGATCAAGGT
GCCGGGCCACACCCGCAGCCATATCGCCTTTCTCGCTGACGGCCACCTGTTCAGCGGTGATACGCTGTTCAGCCTAGGCT
GTGGGCGGATGTTCGAAGGTACCGCTCCCCAGATGTTCGACTCGTTGCAGCGCCTGGCCTCCCTGCCTGGCGAAACGCTT
GTGTGTTGCGGCCACGAATACACGTTGGCCAACGCGGCCTTCGCGCTGCATGTCGATCCCACCAACGCTGCCTTGCAGCG
CCGTCAACAGGAAGCCCAGGCCATGCGTCATGCAGCCCGTCCTACCCTGCCGATTTCGCTCAAGAGTGAACTGGCCACCA
ATCCGTTCTTACGCACCAGCCACCCCGAGATCCGCGCTGCAGTGGCACCACGCGCAGCCAGCGCACTTTCCTCTGAAGTG
GATATTTTTGCCGAACTTCGGCGCTGGAAAGACGAATTTCGGGCATGA

Upstream 100 bases:

>100_bases
GACGCTCCAGCTGAAGCAGGCCGCGCCCCGCGCCGGTTTCAAACCAGGATGAGACGCCAGCTTGACGGGAGAATGGCGGG
GCAGGCATGGTCCCAATTCT

Downstream 100 bases:

>100_bases
GGCGCCTGGTTTTGGCAGTGCTGGCCGCGTGTTCGGCCATTGCACCGGCAGCCGCATCGCCCAACGACGCAAGCTCCGCC
TCCGCAGGTGGCCAGGCCGC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRLIALPAFDDNYIWALVAADGRAIIVDPGQAEPVLAAAQRQGLVPSAVLLTHHHGDHIGGVAELQQRWPDLALFGPADE
RIPTNAHHVGRGERLRLLDVEFQVIKVPGHTRSHIAFLADGHLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETL
VCCGHEYTLANAAFALHVDPTNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTSHPEIRAAVAPRAASALSSEV
DIFAELRRWKDEFRA

Sequences:

>Translated_255_residues
MRLIALPAFDDNYIWALVAADGRAIIVDPGQAEPVLAAAQRQGLVPSAVLLTHHHGDHIGGVAELQQRWPDLALFGPADE
RIPTNAHHVGRGERLRLLDVEFQVIKVPGHTRSHIAFLADGHLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETL
VCCGHEYTLANAAFALHVDPTNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTSHPEIRAAVAPRAASALSSEV
DIFAELRRWKDEFRA
>Mature_255_residues
MRLIALPAFDDNYIWALVAADGRAIIVDPGQAEPVLAAAQRQGLVPSAVLLTHHHGDHIGGVAELQQRWPDLALFGPADE
RIPTNAHHVGRGERLRLLDVEFQVIKVPGHTRSHIAFLADGHLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETL
VCCGHEYTLANAAFALHVDPTNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTSHPEIRAAVAPRAASALSSEV
DIFAELRRWKDEFRA

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=35.361216730038, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=35.361216730038, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI116642887, Length=266, Percent_Identity=33.8345864661654, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI21703352, Length=266, Percent_Identity=33.8345864661654, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI14150041, Length=233, Percent_Identity=38.6266094420601, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI46361987, Length=202, Percent_Identity=39.6039603960396, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1786406, Length=253, Percent_Identity=45.4545454545455, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1787158, Length=190, Percent_Identity=30.5263157894737, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=255, Percent_Identity=34.9019607843137, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320478, Length=249, Percent_Identity=30.5220883534137, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324614, Length=271, Percent_Identity=30.2583025830258, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24667711, Length=265, Percent_Identity=37.3584905660377, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI21356335, Length=265, Percent_Identity=37.3584905660377, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24667703, Length=265, Percent_Identity=37.3584905660377, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_XANAC (Q8PNI0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641426.1
- HSSP:   Q9SID3
- ProteinModelPortal:   Q8PNI0
- SMR:   Q8PNI0
- GeneID:   1155158
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1087
- NMPDR:   fig|190486.1.peg.1070
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK497122
- BioCyc:   XAXO190486:XAC1087-MONOMER
- BRENDA:   3.1.2.6
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 27887; Mature: 27887

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIALPAFDDNYIWALVAADGRAIIVDPGQAEPVLAAAQRQGLVPSAVLLTHHHGDHIG
CEEEEEECCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCC
GVAELQQRWPDLALFGPADERIPTNAHHVGRGERLRLLDVEFQVIKVPGHTRSHIAFLAD
CHHHHHHHCCCEEEECCCCCCCCCCCHHCCCCCEEEEEEEEEEEEECCCCCCCEEEEEEC
GHLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETLVCCGHEYTLANAAFALHVDP
CEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCEEEEEEECC
TNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTSHPEIRAAVAPRAASALSSEV
CHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCEECCCCHHHHHHCCHHHHHHHHHH
DIFAELRRWKDEFRA
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLIALPAFDDNYIWALVAADGRAIIVDPGQAEPVLAAAQRQGLVPSAVLLTHHHGDHIG
CEEEEEECCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCC
GVAELQQRWPDLALFGPADERIPTNAHHVGRGERLRLLDVEFQVIKVPGHTRSHIAFLAD
CHHHHHHHCCCEEEECCCCCCCCCCCHHCCCCCEEEEEEEEEEEEECCCCCCCEEEEEEC
GHLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETLVCCGHEYTLANAAFALHVDP
CEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCEEEEEEECC
TNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTSHPEIRAAVAPRAASALSSEV
CHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCEECCCCHHHHHHCCHHHHHHHHHH
DIFAELRRWKDEFRA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217