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The map label for this gene is rsuA [C]

Identifier: 21241779

GI number: 21241779

Start: 1170385

End: 1171179

Strand: Direct

Name: rsuA [C]

Synonym: XAC1014

Alternate gene names: 21241779

Gene position: 1170385-1171179 (Clockwise)

Preceding gene: 21241778

Following gene: 21241782

Centisome position: 22.61

GC content: 69.18

Gene sequence:

>795_bases
GTGCGCCCACCCGCTGCCGCATCCGCCAATGCCGCGCCACGCCATGGGCTGGCGCGCGTGCTGTCCAAGCTTGGCGTGTG
TTCGCGCACCGAAGCGGCGCGCTGGATTGCCGCTGGCCGCGTCAGCGTGTCGGCACGTGTGGTGCGCGACCCCGAGTTTC
CGATCCGCGGCGATCAGCAATCGTCCATCGCTGTCGATGGGCAACCGCTTGCGGGACTGGCACGCTGCTACCTGATGCTC
AACAAACCGCGCGGCGTGGTCACCACCGTGCGCGACGAGCAGGGCAGGGACACGGTCTACCGTTGCTTCGATGACGCCGG
CCTACCGTGGATCGCGCCGGTGGGGCGCCTGGACAAGGCCAGCGAAGGCCTGTTGCTTTTCAGCAACGACCCACAGTGGG
CGGCGACGGTGACCGACCCGGCGACCGGGCCGGACAAGACCTACCACGTGCAGATCGATTGCCGTCCCAGTGCCGCGCAA
CTGGTGCAGCTGCATGCCGGTGTGGTCGATCCCGATCCACAGGGCCAGGGCCTACTGTTGCGCGCCAAGCAGGTCAGCGT
ACTGCGCGAAGGCGAGCGCAATGCCTGGCTGGAGATCGTGCTGGACGAAGGGCGCAATCGGCAGATCCGCCGGCTGTTGG
CCGCAGTCGACATCGGCGTACTGCGGCTGGTGCGCGTGGCGATCGGCCCGCTGGCGATGGGGGAGCTGGGCAAAGGCGCG
TGGCGGATGCTGAGCGCCGAGGAAGTCAGTGCGTTGGTGCCGGCGGCGCACGCCAGCGCGCCAGACGCGCGCTGA

Upstream 100 bases:

>100_bases
GTCGAAAACAAGTAAGCCCTGCGGTCGGGACGGCATGCCCCGGCGACCTGCGCCGGCAACGTCCCGGCAGCGTCAGCGTG
GTCCGGGGGCGGGCGGCGCG

Downstream 100 bases:

>100_bases
GCAGGGCGCTGACCACGTCGTAAGCGCGGGCGTCGCCGGCGCCGTTGGCATCGTGGCGGCGACCCTTGGCGTGATTGCAG
CCGGCACAGGCCAGCGCCAG

Product: pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRPPAAASANAAPRHGLARVLSKLGVCSRTEAARWIAAGRVSVSARVVRDPEFPIRGDQQSSIAVDGQPLAGLARCYLML
NKPRGVVTTVRDEQGRDTVYRCFDDAGLPWIAPVGRLDKASEGLLLFSNDPQWAATVTDPATGPDKTYHVQIDCRPSAAQ
LVQLHAGVVDPDPQGQGLLLRAKQVSVLREGERNAWLEIVLDEGRNRQIRRLLAAVDIGVLRLVRVAIGPLAMGELGKGA
WRMLSAEEVSALVPAAHASAPDAR

Sequences:

>Translated_264_residues
MRPPAAASANAAPRHGLARVLSKLGVCSRTEAARWIAAGRVSVSARVVRDPEFPIRGDQQSSIAVDGQPLAGLARCYLML
NKPRGVVTTVRDEQGRDTVYRCFDDAGLPWIAPVGRLDKASEGLLLFSNDPQWAATVTDPATGPDKTYHVQIDCRPSAAQ
LVQLHAGVVDPDPQGQGLLLRAKQVSVLREGERNAWLEIVLDEGRNRQIRRLLAAVDIGVLRLVRVAIGPLAMGELGKGA
WRMLSAEEVSALVPAAHASAPDAR
>Mature_264_residues
MRPPAAASANAAPRHGLARVLSKLGVCSRTEAARWIAAGRVSVSARVVRDPEFPIRGDQQSSIAVDGQPLAGLARCYLML
NKPRGVVTTVRDEQGRDTVYRCFDDAGLPWIAPVGRLDKASEGLLLFSNDPQWAATVTDPATGPDKTYHVQIDCRPSAAQ
LVQLHAGVVDPDPQGQGLLLRAKQVSVLREGERNAWLEIVLDEGRNRQIRRLLAAVDIGVLRLVRVAIGPLAMGELGKGA
WRMLSAEEVSALVPAAHASAPDAR

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=243, Percent_Identity=31.6872427983539, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=34.8314606741573, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787524, Length=248, Percent_Identity=34.2741935483871, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=27.6679841897233, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 28334; Mature: 28334

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPAAASANAAPRHGLARVLSKLGVCSRTEAARWIAAGRVSVSARVVRDPEFPIRGDQQ
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCEEEEEEEEECCCCCCCCCCC
SSIAVDGQPLAGLARCYLMLNKPRGVVTTVRDEQGRDTVYRCFDDAGLPWIAPVGRLDKA
CCEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
SEGLLLFSNDPQWAATVTDPATGPDKTYHVQIDCRPSAAQLVQLHAGVVDPDPQGQGLLL
CCCEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEE
RAKQVSVLREGERNAWLEIVLDEGRNRQIRRLLAAVDIGVLRLVRVAIGPLAMGELGKGA
EHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHH
WRMLSAEEVSALVPAAHASAPDAR
HHHCCHHHHHHHHCHHCCCCCCCC
>Mature Secondary Structure
MRPPAAASANAAPRHGLARVLSKLGVCSRTEAARWIAAGRVSVSARVVRDPEFPIRGDQQ
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCEEEEEEEEECCCCCCCCCCC
SSIAVDGQPLAGLARCYLMLNKPRGVVTTVRDEQGRDTVYRCFDDAGLPWIAPVGRLDKA
CCEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
SEGLLLFSNDPQWAATVTDPATGPDKTYHVQIDCRPSAAQLVQLHAGVVDPDPQGQGLLL
CCCEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEE
RAKQVSVLREGERNAWLEIVLDEGRNRQIRRLLAAVDIGVLRLVRVAIGPLAMGELGKGA
EHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHH
WRMLSAEEVSALVPAAHASAPDAR
HHHCCHHHHHHHHCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA