Click here to switch to the map view.

The map label for this gene is lolB

Identifier: 21241717

GI number: 21241717

Start: 1110291

End: 1110944

Strand: Direct

Name: lolB

Synonym: XAC0947

Alternate gene names: 21241717

Gene position: 1110291-1110944 (Clockwise)

Preceding gene: 21241716

Following gene: 21241718

Centisome position: 21.45

GC content: 72.63

Gene sequence:

>654_bases
ATGAGCAAGACGGTCCGAACGCTGGCCCTGGGCGGGCTGGTGCTGGCCGGGCTGTCGGCCTGCGTCAGCGTGCCGCGCGG
GCAGGGCAGCGGTGCGGCGGTGGTCGATCACGTCTCCGACAGTGCGCGCCAGGCCGAAGCCGCTCGCCAGGCCTGGTTGC
AGGCGCATCCCCACTGGTCCTTCCAGGGCCGGGTGGCGATCAGCAAGGACCGCAACGGCGGCAGCGGCCGCATCGACTGG
CAGCAGGATGGCCCGCGCTACCGCGTGCAACTCAGTGCGCCGGTCACCCGGCAAAGCTGGGTGCTCACCGGCGACACCAC
CAGTGGCGCCGGCCGGCTGGAAGGGCTGGAGGGCGGTCCGCGCAGTGGCCCCGATGCCGAGCAGGTGCTGCTGGAAGCAA
CCGGCTGGACCATCCCGGTCAACCAGATGCCGGACTGGGTGCGCGCGCTGCGCATCGCCGGTGCGGGTGCCGCACGCGTC
GACCTGGATGCCGCCGGGCGCCCGCGCACCGTGCAGCAGGACGGCTGGACGATCGACTTTCTGGCCTGGACGCCAGCCAG
CGCCGAGCAGCCCGAGCTGCCGCAGCGGATCGAAGCCCGTAGCGGCGAGGCCAAGGTGCGCCTGCTGGTCGACCAATGGA
CCGTATCGCCCTGA

Upstream 100 bases:

>100_bases
TTAGCGGCAAGCAGGACGAAGCACGTCGCTACTTCGATGAAGCGCGCCGTCTCGACCCCGACAACCGTGCGCTGCAGCGT
GCGGTGGAGAAGTTCGGGCT

Downstream 100 bases:

>100_bases
TGGACGCGCCGGACACGGACTGGTCGGCCTGGCCTGCGCCGGCCAAGCTGAATTTGTTTCTGCAAATCACCGGGCGCCGG
GCCGACGGCTACCACCTGCT

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSKTVRTLALGGLVLAGLSACVSVPRGQGSGAAVVDHVSDSARQAEAARQAWLQAHPHWSFQGRVAISKDRNGGSGRIDW
QQDGPRYRVQLSAPVTRQSWVLTGDTTSGAGRLEGLEGGPRSGPDAEQVLLEATGWTIPVNQMPDWVRALRIAGAGAARV
DLDAAGRPRTVQQDGWTIDFLAWTPASAEQPELPQRIEARSGEAKVRLLVDQWTVSP

Sequences:

>Translated_217_residues
MSKTVRTLALGGLVLAGLSACVSVPRGQGSGAAVVDHVSDSARQAEAARQAWLQAHPHWSFQGRVAISKDRNGGSGRIDW
QQDGPRYRVQLSAPVTRQSWVLTGDTTSGAGRLEGLEGGPRSGPDAEQVLLEATGWTIPVNQMPDWVRALRIAGAGAARV
DLDAAGRPRTVQQDGWTIDFLAWTPASAEQPELPQRIEARSGEAKVRLLVDQWTVSP
>Mature_216_residues
SKTVRTLALGGLVLAGLSACVSVPRGQGSGAAVVDHVSDSARQAEAARQAWLQAHPHWSFQGRVAISKDRNGGSGRIDWQ
QDGPRYRVQLSAPVTRQSWVLTGDTTSGAGRLEGLEGGPRSGPDAEQVLLEATGWTIPVNQMPDWVRALRIAGAGAARVD
LDAAGRPRTVQQDGWTIDFLAWTPASAEQPELPQRIEARSGEAKVRLLVDQWTVSP

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_XANAC (Q8PNU2)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641299.1
- ProteinModelPortal:   Q8PNU2
- SMR:   Q8PNU2
- GeneID:   1155018
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0947
- NMPDR:   fig|190486.1.peg.943
- HOGENOM:   HBG701397
- OMA:   RIEASNG
- ProtClustDB:   PRK00022
- BioCyc:   XAXO190486:XAC0947-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 23201; Mature: 23070

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTVRTLALGGLVLAGLSACVSVPRGQGSGAAVVDHVSDSARQAEAARQAWLQAHPHWS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHCCCCCC
FQGRVAISKDRNGGSGRIDWQQDGPRYRVQLSAPVTRQSWVLTGDTTSGAGRLEGLEGGP
CCCEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCC
RSGPDAEQVLLEATGWTIPVNQMPDWVRALRIAGAGAARVDLDAAGRPRTVQQDGWTIDF
CCCCCHHHEEEEECCCEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCEEEE
LAWTPASAEQPELPQRIEARSGEAKVRLLVDQWTVSP
EEECCCCCCCCCCCHHHHCCCCCEEEEEEEEEEECCC
>Mature Secondary Structure 
SKTVRTLALGGLVLAGLSACVSVPRGQGSGAAVVDHVSDSARQAEAARQAWLQAHPHWS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHCCCCCC
FQGRVAISKDRNGGSGRIDWQQDGPRYRVQLSAPVTRQSWVLTGDTTSGAGRLEGLEGGP
CCCEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCC
RSGPDAEQVLLEATGWTIPVNQMPDWVRALRIAGAGAARVDLDAAGRPRTVQQDGWTIDF
CCCCCHHHEEEEECCCEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCEEEE
LAWTPASAEQPELPQRIEARSGEAKVRLLVDQWTVSP
EEECCCCCCCCCCCHHHHCCCCCEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12024217