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The map label for this gene is thyA

Identifier: 21241612

GI number: 21241612

Start: 1002519

End: 1003313

Strand: Direct

Name: thyA

Synonym: XAC0842

Alternate gene names: 21241612

Gene position: 1002519-1003313 (Clockwise)

Preceding gene: 21241611

Following gene: 21241613

Centisome position: 19.37

GC content: 62.64

Gene sequence:

>795_bases
GTGAAGCCGTACCTGGAGTTGTTGCAGCACGTGCTGGACCACGGCGCAGAGAAGTCCGACCGCACCGGTACCGGCACGCG
CAGCGTGTTCGGCTGGCAGATGCGCTTCGATCTCAACGACGGCTTTCCGTTGGTGACCACCAAGAAGCTGCACCTGCGCT
CGATCATCCATGAGTTGCTGTGGTTCCTGAAGGGCGACACCAATATCGGCTACCTCAGCGACCACCAGGTGCGCATCTGG
GACGAATGGGCCGACGACAACGGCGATCTCGGTCCGGTGTACGGCAAGCAGTGGCGGCGCTGGACCGGCCCGGACGGTGT
CGAGATCGACCAGATGCAGTGGCTGGTGGATGAGATCAAGCGCAATCCGGATTCGCGTCGTCTGGTGATCAGCGCCTGGA
ATGTCGGCGAGCTGCCGCAGATGGCGCTGATGCCTTGCCACAGCCTGTTCCAGTTCTACGTGGTGAACGGCAAGCTCAGT
TGCCAGCTCTATCAGCGCAGCGGCGATATCTTTCTGGGCGTGCCGTTCAATATCGCCAGCTATGCGCTGCTGACGCACAT
GGTGGCCCAGGCCACCGGCCTGGGCGTTGGCGATTTCGTGCACACGCTGGGCGATGCGCACCTGTATTCCAATCACTTCG
AGCAGGCACGCGAGCAGCTGACGCGCACACCGCGTGCGCTGCCCAGCTTGCGTCTGAATCCCGAGGTCACCGACCTGTTC
GCGTTCCGCTTCGAAGACATCGCCATCGAAGGCTACGACCCGCATCCGGCAATCAAGGCCCCGGTGGCGGTGTGA

Upstream 100 bases:

>100_bases
TGCCGTTGGTTGCGGTTGGCCTGGTGCTGCTGGCGATGTCGCGCCGCGCGCCGGTGTTGCAGCCGGTGCTGCCGGTGCCC
GCGGGCGTGGAGGCTGCCAA

Downstream 100 bases:

>100_bases
CGAGCGAGTTGCGGCATGGTCCGCTGGTATCCACTTGAGACGGCAGCACTGTGATGGCCAAGCATTCGATCAAGGCACGG
GAAAAACTGGCTGATTCGCT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKPYLELLQHVLDHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELLWFLKGDTNIGYLSDHQVRIW
DEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIKRNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLS
CQLYQRSGDIFLGVPFNIASYALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFEQAREQLTRTPRALPSLRLNPEVTDLF
AFRFEDIAIEGYDPHPAIKAPVAV

Sequences:

>Translated_264_residues
MKPYLELLQHVLDHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELLWFLKGDTNIGYLSDHQVRIW
DEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIKRNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLS
CQLYQRSGDIFLGVPFNIASYALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFEQAREQLTRTPRALPSLRLNPEVTDLF
AFRFEDIAIEGYDPHPAIKAPVAV
>Mature_264_residues
MKPYLELLQHVLDHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELLWFLKGDTNIGYLSDHQVRIW
DEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIKRNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLS
CQLYQRSGDIFLGVPFNIASYALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFEQAREQLTRTPRALPSLRLNPEVTDLF
AFRFEDIAIEGYDPHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=53.7366548042705, Blast_Score=299, Evalue=1e-81,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=68.5606060606061, Blast_Score=389, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=46.4539007092199, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324648, Length=290, Percent_Identity=38.6206896551724, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=51.5901060070671, Blast_Score=288, Evalue=3e-78,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_XANAC (Q8PP46)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641194.1
- ProteinModelPortal:   Q8PP46
- SMR:   Q8PP46
- GeneID:   1154913
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0842
- NMPDR:   fig|190486.1.peg.838
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   XAXO190486:XAC0842-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30130; Mature: 30130

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYLELLQHVLDHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLKGDTNIGYLSDHQVRIWDEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIK
HHHCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLSCQLYQRSGDIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFEQAREQLTRTPRALPSLRLNPEVTDLF
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHH
AFRFEDIAIEGYDPHPAIKAPVAV
HHEEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKPYLELLQHVLDHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLKGDTNIGYLSDHQVRIWDEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIK
HHHCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLSCQLYQRSGDIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFEQAREQLTRTPRALPSLRLNPEVTDLF
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHH
AFRFEDIAIEGYDPHPAIKAPVAV
HHEEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217