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The map label for this gene is dosC [H]

Identifier: 21241415

GI number: 21241415

Start: 758738

End: 759808

Strand: Direct

Name: dosC [H]

Synonym: XAC0644

Alternate gene names: 21241415

Gene position: 758738-759808 (Clockwise)

Preceding gene: 21241414

Following gene: 21241416

Centisome position: 14.66

GC content: 66.01

Gene sequence:

>1071_bases
TTGAGCCGGCTGGTGTCGGCGCGCTTAGCGTGCGCTGGCGCTCGCCCACCGCGTGCACGGGATCGCTTGTTGAGGCGAAC
CGGTGCCGCACATGGGTACAACCAGCGAGGTCTGAGACTTCCGTCGCGTTGGCTCCACACGCTCTCGGCGACACTAAGCG
CTCTTTTGCCGGAGTGTTGGATGCGCTTGTACGCCGGGCTCTCGCGCGTGTTTCCCCGCTCCTTCAGCGCCAAACTGCTG
GCGGTGACCTTCCTGGGCATCCATCTGCCGTTGCTGTTGTTGATCGCCTGGTTGGCATCGCAGAGCGAACTGGACGGGCG
CCCGCTGTGGTCGGTGGTGATCGTGGCATTGCTGGCCACGCTGGCCGGCACCGCGCTGACCTTGGTTGCGCTGTATCGGC
TGCTGGCGCCGCTGCGCATCGCCGCCGATGCGCTGGATTCGTATTACGCCGATCAGCGCCTGCCCACGCTTCCTGAACAG
GGCGACGACGAATTGGGCCGGCTGTTGCGCGGCATCAACCGCAGCCTGCGTGGCATCGATGCCGGCATGCGCGATCTCAA
GAAACACGCCTTGTTCGACCCGCTGACCGAGGCGCTGAACCGGCGCGGTTGCGAACAGGCGATGCGCGATTCGGTGATCG
CCTCGCAACGCGAAGGCTGGCCGTTCGTGCTGTTCGTGCTGGACATGGACAACCTCAAACAGATCAACGACCGCTTCGGC
CATCTGGCTGGCGACCGCGTGCTGGTGCGCCTGGTGGACTCGGCCTACGGCTGGTTGGGTGCGCAGGACTGGGTCGGGCG
TTGGGGCGGCGACGAATTCCTGATCGGCATCCATGCCAGCGAAGACGACGCCACGCTCAAGCTCAACCAATGGCTCTCGA
TGCTCGAGCGCGAGGATGCCGGCGATGCGCCGCTGCATGTGAGCGCCGGCAGTGCGGTGTGCGAACAAGGGCTGGATGCC
ACCGAACTGTATCGCCGCGCCGATGCGGCGATGTATCGCGCCAAGTTCTCCGGTGGCCGGCGTCTGGTGCGCGACGGGCA
CGACGCTCCGCGCGGCCATCCGGTGATCTGA

Upstream 100 bases:

>100_bases
GGGCATGATGGGCGAGGCCGGGTTTGCGCGTTGCCACTTCAAGAACCTCACCGGCGGCATCGTGGCGATCCACTCCGGCT
ACAAGATCTAAGCAGGGCGT

Downstream 100 bases:

>100_bases
CCTGGGCAAGCCGGTTGCGATACTGAGTGGCAGGTCATCGCTTATGGCCGCCGATGGCACGGGCAGGCTGCGGCGCAGGG
CGCTAAAATGCCCAATTCTC

Product: response regulator

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSRLVSARLACAGARPPRARDRLLRRTGAAHGYNQRGLRLPSRWLHTLSATLSALLPECWMRLYAGLSRVFPRSFSAKLL
AVTFLGIHLPLLLLIAWLASQSELDGRPLWSVVIVALLATLAGTALTLVALYRLLAPLRIAADALDSYYADQRLPTLPEQ
GDDELGRLLRGINRSLRGIDAGMRDLKKHALFDPLTEALNRRGCEQAMRDSVIASQREGWPFVLFVLDMDNLKQINDRFG
HLAGDRVLVRLVDSAYGWLGAQDWVGRWGGDEFLIGIHASEDDATLKLNQWLSMLEREDAGDAPLHVSAGSAVCEQGLDA
TELYRRADAAMYRAKFSGGRRLVRDGHDAPRGHPVI

Sequences:

>Translated_356_residues
MSRLVSARLACAGARPPRARDRLLRRTGAAHGYNQRGLRLPSRWLHTLSATLSALLPECWMRLYAGLSRVFPRSFSAKLL
AVTFLGIHLPLLLLIAWLASQSELDGRPLWSVVIVALLATLAGTALTLVALYRLLAPLRIAADALDSYYADQRLPTLPEQ
GDDELGRLLRGINRSLRGIDAGMRDLKKHALFDPLTEALNRRGCEQAMRDSVIASQREGWPFVLFVLDMDNLKQINDRFG
HLAGDRVLVRLVDSAYGWLGAQDWVGRWGGDEFLIGIHASEDDATLKLNQWLSMLEREDAGDAPLHVSAGSAVCEQGLDA
TELYRRADAAMYRAKFSGGRRLVRDGHDAPRGHPVI
>Mature_355_residues
SRLVSARLACAGARPPRARDRLLRRTGAAHGYNQRGLRLPSRWLHTLSATLSALLPECWMRLYAGLSRVFPRSFSAKLLA
VTFLGIHLPLLLLIAWLASQSELDGRPLWSVVIVALLATLAGTALTLVALYRLLAPLRIAADALDSYYADQRLPTLPEQG
DDELGRLLRGINRSLRGIDAGMRDLKKHALFDPLTEALNRRGCEQAMRDSVIASQREGWPFVLFVLDMDNLKQINDRFGH
LAGDRVLVRLVDSAYGWLGAQDWVGRWGGDEFLIGIHASEDDATLKLNQWLSMLEREDAGDAPLHVSAGSAVCEQGLDAT
ELYRRADAAMYRAKFSGGRRLVRDGHDAPRGHPVI

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=29.940119760479, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787262, Length=180, Percent_Identity=30.5555555555556, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=30.6748466257669, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=31.875, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788956, Length=172, Percent_Identity=29.6511627906977, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=30.7262569832402, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787816, Length=133, Percent_Identity=33.8345864661654, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39442; Mature: 39311

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVSARLACAGARPPRARDRLLRRTGAAHGYNQRGLRLPSRWLHTLSATLSALLPECW
CCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
MRLYAGLSRVFPRSFSAKLLAVTFLGIHLPLLLLIAWLASQSELDGRPLWSVVIVALLAT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LAGTALTLVALYRLLAPLRIAADALDSYYADQRLPTLPEQGDDELGRLLRGINRSLRGID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AGMRDLKKHALFDPLTEALNRRGCEQAMRDSVIASQREGWPFVLFVLDMDNLKQINDRFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
HLAGDRVLVRLVDSAYGWLGAQDWVGRWGGDEFLIGIHASEDDATLKLNQWLSMLEREDA
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCC
GDAPLHVSAGSAVCEQGLDATELYRRADAAMYRAKFSGGRRLVRDGHDAPRGHPVI
CCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SRLVSARLACAGARPPRARDRLLRRTGAAHGYNQRGLRLPSRWLHTLSATLSALLPECW
CHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
MRLYAGLSRVFPRSFSAKLLAVTFLGIHLPLLLLIAWLASQSELDGRPLWSVVIVALLAT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LAGTALTLVALYRLLAPLRIAADALDSYYADQRLPTLPEQGDDELGRLLRGINRSLRGID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AGMRDLKKHALFDPLTEALNRRGCEQAMRDSVIASQREGWPFVLFVLDMDNLKQINDRFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
HLAGDRVLVRLVDSAYGWLGAQDWVGRWGGDEFLIGIHASEDDATLKLNQWLSMLEREDA
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCC
GDAPLHVSAGSAVCEQGLDATELYRRADAAMYRAKFSGGRRLVRDGHDAPRGHPVI
CCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]