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The map label for this gene is bioH

Identifier: 21241159

GI number: 21241159

Start: 456054

End: 456815

Strand: Reverse

Name: bioH

Synonym: XAC0385

Alternate gene names: 21241159

Gene position: 456815-456054 (Counterclockwise)

Preceding gene: 21241160

Following gene: 21241158

Centisome position: 8.83

GC content: 68.64

Gene sequence:

>762_bases
ATGCATATCGATGTCATCGGCCACGGGCCTGCGCTGGTTCTCCTGCACGGTTGGGCACTGCACGGCGGCGTGTTCGCACC
GCTGGTGGAGCGCCTGGCGCCGCACTATCAATTGCATCTGGTCGACCTGCCCGGGCACGGCTTCAGTCGCGACGACAGCA
CACCGCTGGCCTTGCCCTATGTGGTTGCAGAAATTGCGGCCGCCACACCGCCGGCGGTATGGCTGGGCTGGTCGCTGGGC
GGGCTGTTTGCGCTGCATGCGGCCGCCACCCTGCCGCAGGTGCGTGGGTTGGCGATGATTGCCGCCACGCCGCGTTTCGT
GCGTGGCAGCGATTGGCCGAGTGCCGTCCAGCGCGAGGTGTTCGTGCAATTCGGCGTGGAGTTGTCGCGCGACTATCGCG
GCACGCTGGAGCGTTTTCTTGCGCTGGACACGCTCGGCTCGGCGCATGCACGCAGCGAACTGCGCAGCCTGCGCGAGACG
CTGACCGCGCGCGGCGAACCGGCACCGGAAGCGTTGCAGCAAGGGCTCACCCTGCTCGAACGCACCGATCTGCGCCGCAC
CGTGCCGCAGCTCGCACGCCCCAGCCTGTGGATCGCCGGGCAACGCGATCGCCTGGTGCCTGCCGCCGGCATGCATGCCG
CCGCCGCGCTCAGCCCGCACGCGCAGGCGCTCACCATTGCCGGCGGTGGTCACGCGCCGTTTCTTGGTCATGCAGATCAG
GTGAGCGAGGCACTGCAACGCTTCGTTGCGTCCGTGCCATGA

Upstream 100 bases:

>100_bases
CGCCAGGACCCTGCACCCAACATCGGATCTGTGTGCCTCCAATCACCAGCGCGTATCCCGCGATGCGTGCCGGCACGCCA
CAATCGGCGACAATGCGCGC

Downstream 100 bases:

>100_bases
GCCGATCGTCGATCATGCGACATCGCTGATCGAGGGACACTCGCATGGATCTGGGAATCGCTGGCCGCTGGGCGTTGGTC
TGCGCCGCAAGCAAAGGGCT

Product: biotin biosynthesis protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSRDDSTPLALPYVVAEIAAATPPAVWLGWSLG
GLFALHAAATLPQVRGLAMIAATPRFVRGSDWPSAVQREVFVQFGVELSRDYRGTLERFLALDTLGSAHARSELRSLRET
LTARGEPAPEALQQGLTLLERTDLRRTVPQLARPSLWIAGQRDRLVPAAGMHAAAALSPHAQALTIAGGGHAPFLGHADQ
VSEALQRFVASVP

Sequences:

>Translated_253_residues
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSRDDSTPLALPYVVAEIAAATPPAVWLGWSLG
GLFALHAAATLPQVRGLAMIAATPRFVRGSDWPSAVQREVFVQFGVELSRDYRGTLERFLALDTLGSAHARSELRSLRET
LTARGEPAPEALQQGLTLLERTDLRRTVPQLARPSLWIAGQRDRLVPAAGMHAAAALSPHAQALTIAGGGHAPFLGHADQ
VSEALQRFVASVP
>Mature_253_residues
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSRDDSTPLALPYVVAEIAAATPPAVWLGWSLG
GLFALHAAATLPQVRGLAMIAATPRFVRGSDWPSAVQREVFVQFGVELSRDYRGTLERFLALDTLGSAHARSELRSLRET
LTARGEPAPEALQQGLTLLERTDLRRTVPQLARPSLWIAGQRDRLVPAAGMHAAAALSPHAQALTIAGGGHAPFLGHADQ
VSEALQRFVASVP

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=227, Percent_Identity=39.647577092511, Blast_Score=154, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_XANAC (Q8PQE0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640741.1
- ProteinModelPortal:   Q8PQE0
- GeneID:   1154456
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0385
- NMPDR:   fig|190486.1.peg.385
- HOGENOM:   HBG748003
- OMA:   CTIASSP
- ProtClustDB:   CLSK446229
- BioCyc:   XAXO190486:XAC0385-MONOMER
- BRENDA:   3.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 27068; Mature: 27068

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: ACT_SITE 78-78 ACT_SITE 203-203 ACT_SITE 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSRDDSTPLALPY
CEEEEECCCCEEEEEECHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHH
VVAEIAAATPPAVWLGWSLGGLFALHAAATLPQVRGLAMIAATPRFVRGSDWPSAVQREV
HHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHH
FVQFGVELSRDYRGTLERFLALDTLGSAHARSELRSLRETLTARGEPAPEALQQGLTLLE
HHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RTDLRRTVPQLARPSLWIAGQRDRLVPAAGMHAAAALSPHAQALTIAGGGHAPFLGHADQ
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCCCCCHHH
VSEALQRFVASVP
HHHHHHHHHHCCC
>Mature Secondary Structure
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSRDDSTPLALPY
CEEEEECCCCEEEEEECHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHH
VVAEIAAATPPAVWLGWSLGGLFALHAAATLPQVRGLAMIAATPRFVRGSDWPSAVQREV
HHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHH
FVQFGVELSRDYRGTLERFLALDTLGSAHARSELRSLRETLTARGEPAPEALQQGLTLLE
HHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RTDLRRTVPQLARPSLWIAGQRDRLVPAAGMHAAAALSPHAQALTIAGGGHAPFLGHADQ
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCCCCCHHH
VSEALQRFVASVP
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217