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The map label for this gene is gltD [H]

Identifier: 21240806

GI number: 21240806

Start: 37522

End: 39003

Strand: Reverse

Name: gltD [H]

Synonym: XAC0032

Alternate gene names: 21240806

Gene position: 39003-37522 (Counterclockwise)

Preceding gene: 21240807

Following gene: 21240804

Centisome position: 0.75

GC content: 68.15

Gene sequence:

>1482_bases
ATGAGCCGCAAACAAGCCTTCCAATTCCTCGACCTGCCCCGGACCATGCCCACGCGCATTCCGGTGGAACTGCGCACGTC
CGGCGACTGGGGCGAGTTGTACGGCAGGTTCGACAAGGCCGACGCGCAATACCAGGCCGGCCGTTGCCTGGACTGCGGTA
ATCCGTATTGCAGCTGGAAATGCCCGGTGCACAACGCCATCCCGCAATGGCTGCAGCTGGTGCAGGAAGACCGCATCATC
GAGGCCGCCGCGTTGTGCCATTCGACCAACCCGCTGCCGGAAGTGTGCGGGCGGGTGTGTCCGCAGGACCGCCTGTGCGA
AGGCAGCTGCACGCTGGAAGAATTCGGCGCGGTCACCATCGGCGCGGTGGAGAAATACATCGTCGACACCGCCTTCAAGA
TGGGCTGGCGCCCGGACCTGGGCAACGTGCAGGCCAGCGGCCACCGTGTGGCGGTGATCGGCGCCGGCCCGGCCGGCCTG
GCGTGTGCGGACCGCCTGGCGCGCGCCGGTATCGAAGCGGTGGTGTACGACCGCTATGAGCAGATCGGCGGCCTGCTGCA
GTTCGGCATCCCCAGCTTCAAGCTGGACAAATCGGTGATCGGCAAGCGCCGCGAGATCCTCGAAGGCATGGGCGTGCAGT
TCCGCCTGGGCGTGGAGGTCGGCCGCGACGTCAGCATCGAGCAGTTGCTGGGCGAATACGACGCAGTGTTCTTAGGGACC
GGCGCGTATCGCTACACCGACGGCGGCCTGCCCGGGCAGGACCTGAAGAACGTGCTGCCGGCCCTGCCGTTCCTGGTGCA
GAACAGCCGCATCGTCAGCGGCAACGACCCGCACGGCCGGCCGATCGCTGGCTGGGAAGACCAGCTCGCCCTGCCCGACC
TCACCGGCAAGCGCGTGGTGGTGCTGGGCGGTGGCGACACCGGCATGGACTGCGTGCGCAGCGCCATTCGTCTGGGCGCA
GCCAAGGTCACCTGCGCGTATCGCCGCGACGAAGCCAGCATGCCCGGCTCGGCACGCGAGGTTGCCAACGCACGCGAAGA
AGGCGTGCGCTTCCTGTTCAACCGCCAACCGCTGTCGATCGAATCCGGCGCCGACGACGAAGCCATCGGCGTGACCGTAG
TGGAAACCAAGCTGGGCGAACCCGACGCCAGCGGCCGCCGCAATGCGGTGCCGGTGGAAGGCAGCGAATCGCTGCTGGAA
GCGGACGTGGTGATCATCGCCTTCGGCTTCTCGCCTACCCTGCCCGACTGGTTGGCCTCGCAAGGCGTGGACGCCGGCAG
CAATGGCCGCATCGTCGCCCCGTCCGACGGCAACGGCCGCCTGCCCTACCAGACCACCAACCCGCGCCTGTTCGCCGGCG
GCGACTGCGTGCGCGGCGCCGATCTGGTGGTGACCGCAGTAGCCGAAGGCCGCGATGCGGCGGGAAGCATCGTGCAGCTG
CTCGGGGTCAAAGCGCAGGTCAAGGAGCCGGCTGCGGCGTAG

Upstream 100 bases:

>100_bases
GAGGGGCTGGGGGTGAGGGCAGCGGCCTGCCCCCCTCATCCGCCCCTTCGGGGCACCTTCTCCCGCACGCGGGAGAAGCG
AATCAGCTACGGATTTCTTT

Downstream 100 bases:

>100_bases
CCGTCGTGGGTGCACAAGATATGAGATTGCAGGTACCAGAGCAGGCCGTAGCGCTCATTTCCCTCTGACTTCCTTGCAAC
CGTCGCCAGACTCGATCTGG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGRFDKADAQYQAGRCLDCGNPYCSWKCPVHNAIPQWLQLVQEDRII
EAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFKMGWRPDLGNVQASGHRVAVIGAGPAGL
ACADRLARAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEVGRDVSIEQLLGEYDAVFLGT
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLTGKRVVVLGGGDTGMDCVRSAIRLGA
AKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLE
ADVVIIAFGFSPTLPDWLASQGVDAGSNGRIVAPSDGNGRLPYQTTNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
LGVKAQVKEPAAA

Sequences:

>Translated_493_residues
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGRFDKADAQYQAGRCLDCGNPYCSWKCPVHNAIPQWLQLVQEDRII
EAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFKMGWRPDLGNVQASGHRVAVIGAGPAGL
ACADRLARAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEVGRDVSIEQLLGEYDAVFLGT
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLTGKRVVVLGGGDTGMDCVRSAIRLGA
AKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLE
ADVVIIAFGFSPTLPDWLASQGVDAGSNGRIVAPSDGNGRLPYQTTNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
LGVKAQVKEPAAA
>Mature_492_residues
SRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGRFDKADAQYQAGRCLDCGNPYCSWKCPVHNAIPQWLQLVQEDRIIE
AAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFKMGWRPDLGNVQASGHRVAVIGAGPAGLA
CADRLARAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEVGRDVSIEQLLGEYDAVFLGTG
AYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLTGKRVVVLGGGDTGMDCVRSAIRLGAA
KVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEA
DVVIIAFGFSPTLPDWLASQGVDAGSNGRIVAPSDGNGRLPYQTTNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQLL
GVKAQVKEPAAA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=454, Percent_Identity=29.295154185022, Blast_Score=151, Evalue=2e-36,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=57.6763485477178, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1788811, Length=476, Percent_Identity=55.2521008403361, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI87082180, Length=458, Percent_Identity=53.2751091703057, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1788468, Length=452, Percent_Identity=29.8672566371681, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1789243, Length=443, Percent_Identity=32.0541760722348, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17570289, Length=491, Percent_Identity=33.8085539714868, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI71984108, Length=459, Percent_Identity=30.718954248366, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17543860, Length=172, Percent_Identity=29.6511627906977, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=476, Percent_Identity=34.8739495798319, Blast_Score=249, Evalue=8e-67,
Organism=Drosophila melanogaster, GI28574881, Length=477, Percent_Identity=36.6876310272537, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24665539, Length=477, Percent_Identity=36.6876310272537, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24665547, Length=478, Percent_Identity=37.0292887029289, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24665543, Length=478, Percent_Identity=37.0292887029289, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24640763, Length=458, Percent_Identity=30.3493449781659, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18858217, Length=458, Percent_Identity=30.3493449781659, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52682; Mature: 52551

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGRFDKADAQYQAGRCLDCGNPYCSWK
CCHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCEEECCCCCCEEE
CPVHNAIPQWLQLVQEDRIIEAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEH
GAVEKYIVDTAFKMGWRPDLGNVQASGHRVAVIGAGPAGLACADRLARAGIEAVVYDRYE
HHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
QIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEVGRDVSIEQLLGEYDAVFLGT
HHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCEEEEEC
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLTGKRVV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
VLGGGDTGMDCVRSAIRLGAAKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSI
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCCC
ESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEADVVIIAFGFSPTLPDWLAS
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHH
QGVDAGSNGRIVAPSDGNGRLPYQTTNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
CCCCCCCCCCEEECCCCCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
LGVKAQVKEPAAA
HCCHHHCCCCCCC
>Mature Secondary Structure 
SRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGRFDKADAQYQAGRCLDCGNPYCSWK
CHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCEEECCCCCCEEE
CPVHNAIPQWLQLVQEDRIIEAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEH
GAVEKYIVDTAFKMGWRPDLGNVQASGHRVAVIGAGPAGLACADRLARAGIEAVVYDRYE
HHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
QIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEVGRDVSIEQLLGEYDAVFLGT
HHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCEEEEEC
GAYRYTDGGLPGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQLALPDLTGKRVV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
VLGGGDTGMDCVRSAIRLGAAKVTCAYRRDEASMPGSAREVANAREEGVRFLFNRQPLSI
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCCC
ESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEADVVIIAFGFSPTLPDWLAS
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHH
QGVDAGSNGRIVAPSDGNGRLPYQTTNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
CCCCCCCCCCEEECCCCCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
LGVKAQVKEPAAA
HCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]