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The map label for this gene is recF

Identifier: 21240777

GI number: 21240777

Start: 3799

End: 4905

Strand: Direct

Name: recF

Synonym: XAC0003

Alternate gene names: 21240777

Gene position: 3799-4905 (Clockwise)

Preceding gene: 21240776

Following gene: 21240778

Centisome position: 0.07

GC content: 65.4

Gene sequence:

>1107_bases
ATGCATGTAGTGCGGTTGTCCATTCATCGGCTTCGTCGGTTTCAAACCGTCGAGCTTCATCCCTCCAGTGCCTTGAATCT
GCTGACCGGCGACAACGGCGCGGGCAAGACCAGCGTGCTCGAAGCGCTACACCTGATGGCTTACGGCCGCAGCTTCCGCG
GGCGCGTCCGCGACGGCCTGATCCAACAAGGCGCCAACGACCTCGAAGTGTTCGTGGAGTGGAAAGAAGGCGGCGGCGCT
GCGGTCGAGCGGACGCGTCGGGCTGGCTTGCGTCATAGCGGGCAGGAATGGACAGGGCGCCTGGACGGGGAAGACGTGGC
GCAGCTTGGCTCTCTTTGCGCTGCGCTGGCAGTGGTGACGTTCGAGCCCGGCAGCCACGTATTGATCAGTGGCGGTGGTG
AACCCCGCCGCCGTTTTCTGGATTGGGGCCTGTTCCACGTGGAACCCGATTTTCTAACCTTGTGGCGCCGCTATGCGCGA
GCCCTCAAACAACGCAATGCGTTGCTGAAACAGGGCGCGCAGCCACGCATGCTGGACGCATGGGACAACGAACTCGCCGA
ATCCGGCGAAACCCTGACGTCCCGGCGCATGCGCTACCTGGAACGCCTGCAGGACCGGCTGGTTCCCGTGGCAGATGCCA
TCGCGCCGGCGCTGGGACTGTCTGCGCTGACGTTCGCCCCAGGCTGGAAGCGCCACGAGGTCTCGCTGGCAGACGCCCTC
CTGCTTGCGCGTGAGCGTGATCGCCAGAACGGCTATACCTCGCAAGGCCCGCACCGCGCAGATTGGATGCCCAGCTTCCA
CGCACTCCCGGGCAAGGACGCGCTTTCCCGGGGACAGGCCAAGCTCACGGCCCTTGCCTGTCTGCTCGCACAGGCCGAGG
ACTTCGCCTTCGAACGCGGCGAATGGCCGGTCATCGCGCTGGACGATCTGGGCTCGGAGCTGGACCGGCATCATCAGGGG
CGCGTGCTGCAGCGGCTCGCTTCCGCGCCGGCGCAGGTGCTCATCACCGCAACCGAGACCCCACCGGGCCTTGCAGACGC
AGCTGCACTATTGCAGCAGTTCCACGTGGAACACGGCCAGATTGCGCGACAGGCAACGGTCAACTGA

Upstream 100 bases:

>100_bases
TCCGGTCATTCCTGGCGGCCATGTCTGCACGGTTTCATGCCGATCCCGTATCCTTCGAACCGACCGGCATGCCGGATTAC
AGCCCAGAGCACACCGATCG

Downstream 100 bases:

>100_bases
GCCGCGACGGCCGGCACGGGCCGACTGGTATAATTCGTCCGCTATCCCCCTTTTCCCACGGCGCGGAGCGGTCATCGCTG
CCTTCGCCCGTGTTGTGGAG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MHVVRLSIHRLRRFQTVELHPSSALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGLIQQGANDLEVFVEWKEGGGA
AVERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVTFEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLTLWRRYAR
ALKQRNALLKQGAQPRMLDAWDNELAESGETLTSRRMRYLERLQDRLVPVADAIAPALGLSALTFAPGWKRHEVSLADAL
LLARERDRQNGYTSQGPHRADWMPSFHALPGKDALSRGQAKLTALACLLAQAEDFAFERGEWPVIALDDLGSELDRHHQG
RVLQRLASAPAQVLITATETPPGLADAAALLQQFHVEHGQIARQATVN

Sequences:

>Translated_368_residues
MHVVRLSIHRLRRFQTVELHPSSALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGLIQQGANDLEVFVEWKEGGGA
AVERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVTFEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLTLWRRYAR
ALKQRNALLKQGAQPRMLDAWDNELAESGETLTSRRMRYLERLQDRLVPVADAIAPALGLSALTFAPGWKRHEVSLADAL
LLARERDRQNGYTSQGPHRADWMPSFHALPGKDALSRGQAKLTALACLLAQAEDFAFERGEWPVIALDDLGSELDRHHQG
RVLQRLASAPAQVLITATETPPGLADAAALLQQFHVEHGQIARQATVN
>Mature_368_residues
MHVVRLSIHRLRRFQTVELHPSSALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGLIQQGANDLEVFVEWKEGGGA
AVERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVTFEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLTLWRRYAR
ALKQRNALLKQGAQPRMLDAWDNELAESGETLTSRRMRYLERLQDRLVPVADAIAPALGLSALTFAPGWKRHEVSLADAL
LLARERDRQNGYTSQGPHRADWMPSFHALPGKDALSRGQAKLTALACLLAQAEDFAFERGEWPVIALDDLGSELDRHHQG
RVLQRLASAPAQVLITATETPPGLADAAALLQQFHVEHGQIARQATVN

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=363, Percent_Identity=35.2617079889807, Blast_Score=191, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_XANAC (Q8PRG0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640359.1
- ProteinModelPortal:   Q8PRG0
- SMR:   Q8PRG0
- GeneID:   1154074
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0003
- NMPDR:   fig|190486.1.peg.3
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   XAXO190486:XAC0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40642; Mature: 40642

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVVRLSIHRLRRFQTVELHPSSALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGL
CEEEEHHHHHHHHHEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IQQGANDLEVFVEWKEGGGAAVERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVT
HHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHEE
FEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLTLWRRYARALKQRNALLKQGAQPRMLDA
ECCCCEEEEECCCCHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
WDNELAESGETLTSRRMRYLERLQDRLVPVADAIAPALGLSALTFAPGWKRHEVSLADAL
HHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LLARERDRQNGYTSQGPHRADWMPSFHALPGKDALSRGQAKLTALACLLAQAEDFAFERG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EWPVIALDDLGSELDRHHQGRVLQRLASAPAQVLITATETPPGLADAAALLQQFHVEHGQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
IARQATVN
HHHHHCCC
>Mature Secondary Structure
MHVVRLSIHRLRRFQTVELHPSSALNLLTGDNGAGKTSVLEALHLMAYGRSFRGRVRDGL
CEEEEHHHHHHHHHEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IQQGANDLEVFVEWKEGGGAAVERTRRAGLRHSGQEWTGRLDGEDVAQLGSLCAALAVVT
HHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHEE
FEPGSHVLISGGGEPRRRFLDWGLFHVEPDFLTLWRRYARALKQRNALLKQGAQPRMLDA
ECCCCEEEEECCCCHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
WDNELAESGETLTSRRMRYLERLQDRLVPVADAIAPALGLSALTFAPGWKRHEVSLADAL
HHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LLARERDRQNGYTSQGPHRADWMPSFHALPGKDALSRGQAKLTALACLLAQAEDFAFERG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EWPVIALDDLGSELDRHHQGRVLQRLASAPAQVLITATETPPGLADAAALLQQFHVEHGQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
IARQATVN
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217