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The map label for this gene is gyrB [H]

Identifier: 21240778

GI number: 21240778

Start: 5020

End: 7464

Strand: Direct

Name: gyrB [H]

Synonym: XAC0004

Alternate gene names: 21240778

Gene position: 5020-7464 (Clockwise)

Preceding gene: 21240777

Following gene: 21240779

Centisome position: 0.1

GC content: 64.95

Gene sequence:

>2445_bases
ATGACCGACGAACAAAACACCCCGCCAACACCCAACGGCACCTACGACTCCAGCAAGATCACCGTGCTGCGTGGCCTGGA
AGCCGTCCGCAAGCGTCCCGGCATGTATATCGGCGACGTCCATGACGGCACCGGCCTGCATCACATGGTGTTCGAGGTGG
TCGACAACTCGGTCGACGAAGCCCTTGCCGGGCATGCCGACGACATCGTGGTAAAAATCCTGGCCGATGGCTCGGTGGCG
GTCTCCGACAACGGGCGCGGCGTGCCGGTCGACATCCACAAGGAAGAAGGCGTGTCGGCGGCCGAGGTGATCCTCACCGT
GCTCCACGCCGGCGGCAAGTTCGACGACAACAGCTACAAGGTCTCCGGCGGCCTGCACGGCGTTGGCGTCTCGGTGGTCA
ACGCGTTGTCAGAGCACCTGTGGCTGGATATCTGGCGCGACGGCTTCCACTACCAGCAGGAATACGCGCTGGGCGAGCCG
CAGTACCCGCTCAAGCAGCTGGAAGCCTCGACCAAGCGCGGTACCACGCTGCGCTTCAAGCCGTCCGTGGCCATCTTCAG
CGACGTCGAGTTCCATTACGACATCCTGGCGCGGCGCCTGCGCGAGCTGTCCTTCCTCAATTCTGGCGTCAAGATCACCT
TGATCGACGAGCGCGGCGAAGGCCGTCGCGACGATTTCCATTACGAAGGCGGCATCCGCAGCTTCGTGGAGCATCTGGCG
CAGCTGAAGTCGCCGCTGCACCCGAATGTGATCTCGGTGACCGGCGAGCACAACGGCATCATGGTGGACGTGGCCCTGCA
ATGGACCGACGCCTACCAGGAAACCATGTACTGCTTCACCAACAACATCCCGCAGAAGGACGGCGGCACCCACCTGGCCG
GCTTCCGTGCGGCGCTGACGCGCGTGCTCAGCACCTACATCGAACAGAACGGCATCGCCAAGCAGGCCAAGGTCGCGCTG
ACCGGCGACGACATGCGCGAAGGCATGATCGCGGTGCTTTCGGTCAAGGTGCCCGACCCCAGCTTCTCTTCGCAGACCAA
GGAAAAGCTGGTCAGTTCGGATGTGCGCCCGGCGGTGGAAAACGCCTTCGGTGCGCGCCTGCAGGAGTTCCTGCAGGAGA
ACCCGAACGAAGCCAAGGCCATCACCGGCAAGATCGTCGATGCCGCGCGTGCGCGCGAAGCGGCGCGCAAGGCGCGCGAC
CTCACCCGCCGCAAGGGTGCGCTGGACATCGCAGGCCTGCCCGGCAAGCTGGCCGACTGCCAGGAAAAGGATCCGGCACT
GTCGGAACTGTTCATCGTCGAGGGTGACTCGGCAGGTGGCTCGGCCAAGCAGGGCCGCAACCGCAAGAACCAGGCGGTGC
TGCCGCTGCGCGGCAAGATCCTCAACGTGGAACGCGCCCGCTTCGACCGCATGCTGGCCTCCGACCAGGTCGGCACGCTG
ATCACCGCGCTCGGTACCGGCATCGGCCGCGACGAGTACAACCCGGACAAGCTGCGCTACCACCGCATCATCCTGATGAC
CGACGCCGACGTCGACGGCTCGCACATCCGCACCCTGCTGCTGACCTTCTTCTACCGGCAGATGCCGGAGTTGATCGAGC
GCGGCTACATCTACATCGGCCTGCCGCCGCTGTACAAGCTCAAGCAGGGCAAGAGCGAGCTGTATCTGAAGGACGACGCG
GCGCTCAACGCCTACCTGGCCAGCAATGCGGTCGAAGGTGCGGCGCTGATTCCGGCCACCGACGAGCCACCGATCACCGG
CGAAGCGCTGGAGAAATTGTTGATGCTGTTCACCAGCGCCAACGAAGCGATCGCGCGCAACGCCCACCGCTACGACCCGG
CCCTGCTCACCGCGCTGATCGACCTGCCGCCACTGGATGTGGAAAAACTGCAGGCCGAAGGCGACCAGCATCCGACTTTG
GATGCGCTGCAGGCCGTGCTCAATCGCGGCACCCTGGGCACCGCGCGCTATCAGTTGCGCTTCGACCCGGGCAGCGACAA
CGCGCCTGCCACGTTGGTGGCCATCCGCCGCCACATGGGCGAAGAGTTCACCCAGGTGCTGCCGATGGGGGCCTTCGAAA
GCGGCGAGCTGCGCCCGCTGCGTGAGGTGTCGCTGGCCCTGCACGACCTGGTGCGCGAAGGCGCCCAGATCGTCCGCGGC
AACAAGAGCCACCCGATCACCAGCTTCGCGCAGGCCCATGCCTGGTTGCTGGACGAGGCCAAGAAGGGCCGCCAGGTGCA
GCGCTTCAAGGGCCTGGGCGAAATGAATGCCGAGCAGCTATGGGAAACCACGGTCAACCCCGACACGCGTCGCCTGTTGC
AGGTGCGCATCGAGGACGCGGTGGCCGCCGACCAGATCTTCAGCACCTTGATGGGCGATGTGGTCGAACCACGCCGCGAC
TTCATCGAGGACAACGCGCTGAAGGTGTCCAACCTGGATATCTGA

Upstream 100 bases:

>100_bases
CACGGGCCGACTGGTATAATTCGTCCGCTATCCCCCTTTTCCCACGGCGCGGAGCGGTCATCGCTGCCTTCGCCCGTGTT
GTGGAGCCAACGGCACGCGC

Downstream 100 bases:

>100_bases
GTCACCAGCACCGGCCGGTACGTCGCAACCGCGGCGTGCCGGCCGGTGCGGTGCTCGCAGGACGACGATGCGCACCGATC
TTCCACCGCCCCTGGCACCA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 814; Mature: 813

Protein sequence:

>814_residues
MTDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHADDIVVKILADGSVA
VSDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEP
QYPLKQLEASTKRGTTLRFKPSVAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLA
QLKSPLHPNVISVTGEHNGIMVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALTRVLSTYIEQNGIAKQAKVAL
TGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVENAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARD
LTRRKGALDIAGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTL
ITALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIGLPPLYKLKQGKSELYLKDDA
ALNAYLASNAVEGAALIPATDEPPITGEALEKLLMLFTSANEAIARNAHRYDPALLTALIDLPPLDVEKLQAEGDQHPTL
DALQAVLNRGTLGTARYQLRFDPGSDNAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRG
NKSHPITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDAVAADQIFSTLMGDVVEPRRD
FIEDNALKVSNLDI

Sequences:

>Translated_814_residues
MTDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHADDIVVKILADGSVA
VSDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEP
QYPLKQLEASTKRGTTLRFKPSVAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLA
QLKSPLHPNVISVTGEHNGIMVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALTRVLSTYIEQNGIAKQAKVAL
TGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVENAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARD
LTRRKGALDIAGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTL
ITALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIGLPPLYKLKQGKSELYLKDDA
ALNAYLASNAVEGAALIPATDEPPITGEALEKLLMLFTSANEAIARNAHRYDPALLTALIDLPPLDVEKLQAEGDQHPTL
DALQAVLNRGTLGTARYQLRFDPGSDNAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRG
NKSHPITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDAVAADQIFSTLMGDVVEPRRD
FIEDNALKVSNLDI
>Mature_813_residues
TDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHADDIVVKILADGSVAV
SDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEPQ
YPLKQLEASTKRGTTLRFKPSVAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLAQ
LKSPLHPNVISVTGEHNGIMVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALTRVLSTYIEQNGIAKQAKVALT
GDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVENAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARDL
TRRKGALDIAGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTLI
TALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIGLPPLYKLKQGKSELYLKDDAA
LNAYLASNAVEGAALIPATDEPPITGEALEKLLMLFTSANEAIARNAHRYDPALLTALIDLPPLDVEKLQAEGDQHPTLD
ALQAVLNRGTLGTARYQLRFDPGSDNAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRGN
KSHPITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDAVAADQIFSTLMGDVVEPRRDF
IEDNALKVSNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=595, Percent_Identity=26.5546218487395, Blast_Score=150, Evalue=6e-36,
Organism=Homo sapiens, GI19913406, Length=571, Percent_Identity=25.0437828371278, Blast_Score=138, Evalue=3e-32,
Organism=Escherichia coli, GI48994957, Length=808, Percent_Identity=61.6336633663366, Blast_Score=972, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=547, Percent_Identity=40.9506398537477, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535065, Length=582, Percent_Identity=25.4295532646048, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=510, Percent_Identity=24.7058823529412, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=154, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=585, Percent_Identity=25.982905982906, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17136538, Length=584, Percent_Identity=24.8287671232877, Blast_Score=136, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89659; Mature: 89528

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHH
ALAGHADDIVVKILADGSVAVSDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYK
HHCCCCCCEEEEEEECCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEPQYPLKQLEASTKRGTTLRFK
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHCCCCEEEEC
PSVAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLA
CCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
QLKSPLHPNVISVTGEHNGIMVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALT
HHHCCCCCCEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RVLSTYIEQNGIAKQAKVALTGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVE
HHHHHHHHHCCCCCCEEEEECCCHHHCCEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHH
NAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARDLTRRKGALDIAGLPGKLADC
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
QEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTL
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHH
ITALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIG
HHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEE
LPPLYKLKQGKSELYLKDDAALNAYLASNAVEGAALIPATDEPPITGEALEKLLMLFTSA
CCCHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCC
NEAIARNAHRYDPALLTALIDLPPLDVEKLQAEGDQHPTLDALQAVLNRGTLGTARYQLR
CHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEE
FDPGSDNAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRG
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
NKSHPITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDA
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
VAADQIFSTLMGDVVEPRRDFIEDNALKVSNLDI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
>Mature Secondary Structure 
TDEQNTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHH
ALAGHADDIVVKILADGSVAVSDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYK
HHCCCCCCEEEEEEECCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEPQYPLKQLEASTKRGTTLRFK
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHCCCCEEEEC
PSVAIFSDVEFHYDILARRLRELSFLNSGVKITLIDERGEGRRDDFHYEGGIRSFVEHLA
CCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
QLKSPLHPNVISVTGEHNGIMVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALT
HHHCCCCCCEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RVLSTYIEQNGIAKQAKVALTGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVE
HHHHHHHHHCCCCCCEEEEECCCHHHCCEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHH
NAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARDLTRRKGALDIAGLPGKLADC
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
QEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTL
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHH
ITALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIG
HHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEE
LPPLYKLKQGKSELYLKDDAALNAYLASNAVEGAALIPATDEPPITGEALEKLLMLFTSA
CCCHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCC
NEAIARNAHRYDPALLTALIDLPPLDVEKLQAEGDQHPTLDALQAVLNRGTLGTARYQLR
CHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEE
FDPGSDNAPATLVAIRRHMGEEFTQVLPMGAFESGELRPLREVSLALHDLVREGAQIVRG
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
NKSHPITSFAQAHAWLLDEAKKGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDA
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
VAADQIFSTLMGDVVEPRRDFIEDNALKVSNLDI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]