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The map label for this gene is ctsE [H]

Identifier: 57238494

GI number: 57238494

Start: 1543718

End: 1545277

Strand: Reverse

Name: ctsE [H]

Synonym: CJE1644

Alternate gene names: 57238494

Gene position: 1545277-1543718 (Counterclockwise)

Preceding gene: 57238495

Following gene: 57238493

Centisome position: 86.92

GC content: 29.23

Gene sequence:

>1560_bases
ATGGAAAGTAGAATGGATAAAATTTTTCAAGCTTATATTGATAATGAAATAAGTCTTGATGAAATTTGCACTAAATTTAA
TATCACTTCTTGGGATTTTTTTAACAAATTAGCAAATTTTTGTAATTTACATTTTGTTGATTTAGATGAAGATAATGATT
TTATTTATGAAGGTATACCTTTTTCTTTACTTTTAAAATTTAAATTTCTGCTTATTAAAAACAATGACGGCTTTATGATT
ATTAGATCCAAACCTTGTTCTTTAGAGCTTTTAGAGCAAGTTAAGACTTTTATGATTTGTGAAAAAATCGACACTGCAAT
TGCCGATGAATTAAAGATAGCTAAAATTTTAAATCAAATTCGCATTCAAGAGGAGATTAAAAGATTAAGCATTAAGCTTA
GACTTGAATGGCAAGAAAATTACAAAAGAGATGATCAAAGTTGCATCAGTCAAATTTTTGATTTTTTATTACATGAAATT
TTATCTTTTCATGCGAGTGATATTCATATAGAGGCAAGAAATGATGATGCTTTGATTAGGTTTAGGGTTGATGGAATTTT
GCGTGAATTTGCTATTTTTGAGAAGGATATTTATGAAGCTTTGGTTTTTCATGTCAAATTTTTAGCTTGTTTGAATTTAG
CTGAATCAAGAAAAACACAAGATGGAAGTTTTGAATTAGATTTTGAAAATGAAAGATATGATTTTCGTGTTTCATGTTTG
CCTTTGATTTATGGAGAAAGTGTTGTTATAAGAATTTTAAAACATGATAAAGAAATTTTGGATTTGTATAAACTAAATCT
TGGAAATAAAAATTTAGAAATTTTAAAAAAGATTTTACATCGTCCCAATGGAATGATTTTATTAACCGGTCCTACAGGTA
GTGGTAAAAGCACAACTTTATACGCGTGTTTAAATGAGTTGAAAAGTATTGAAAAAAAGATTATTTCCGCAGAAGATCCT
ATAGAATATAAAATTCCTTTAGTGCAGCAAATTTTGCTCAATTCAAAAGTTGGAGTCGAGTTTAATAGTGTTTTAAGAGC
AATTTTAAGGCAAGATCCTGATATTATCATGATAGGAGAAATTCGCGATGAAGAAAGTTTAGATATTGCACTTAAAGCTT
CTTTAACAGGTCATTTGCTTTTAAGCACCTTGCATACTAATGATGCTCTTAGTACCATTGATAGGCTTTTGGATATGCAA
GCAAAAAGTTACTTGATAGCATCGGCTTTGAGTCTTGTTATAGCACAGCGTTTGGTTAGAAAACTTTGTCCTTGGTGTAA
GCAAAAAAGCAAAAAACATTATATTGAATTTGAAGGTGAATTTTTTGAACCAAAAGGATGTGAGCGTTGTCATCATAGTG
GATTTTTTGGCAGAGAATTAATCGCTGAGTGCTTAGAGATCAATGAAGATTTAGCTTGTGCAATTAGGGAAAATCAAGAT
AAAACGATTTTAATGGAGCTTGCAAAAAAATATGGCTTTCAAACTATGTTTGAACAAGGTTTAAAAAAAGCCAAAGAAGG
TCTTACAAGTATAGATGAGCTTTTAAGGGTTGTAAGATGA

Upstream 100 bases:

>100_bases
TGGCTGATTTTTGCTCAGGCTAAAAGAGCTTTAGGCGAAGAAAAAGAAGCTCAAAGTGCTTTAGATGCTTATATAAATTA
TTATGGTTTAATTGAATTAG

Downstream 100 bases:

>100_bases
AATTTTATGAAGTGGAATTTCTTAAAAACAATCAAAATTATACAAAAACTATAAAAGCTGAAAATTTAAATACAGCACAA
GCAAAAGCCTTATCAAAGAA

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MESRMDKIFQAYIDNEISLDEICTKFNITSWDFFNKLANFCNLHFVDLDEDNDFIYEGIPFSLLLKFKFLLIKNNDGFMI
IRSKPCSLELLEQVKTFMICEKIDTAIADELKIAKILNQIRIQEEIKRLSIKLRLEWQENYKRDDQSCISQIFDFLLHEI
LSFHASDIHIEARNDDALIRFRVDGILREFAIFEKDIYEALVFHVKFLACLNLAESRKTQDGSFELDFENERYDFRVSCL
PLIYGESVVIRILKHDKEILDLYKLNLGNKNLEILKKILHRPNGMILLTGPTGSGKSTTLYACLNELKSIEKKIISAEDP
IEYKIPLVQQILLNSKVGVEFNSVLRAILRQDPDIIMIGEIRDEESLDIALKASLTGHLLLSTLHTNDALSTIDRLLDMQ
AKSYLIASALSLVIAQRLVRKLCPWCKQKSKKHYIEFEGEFFEPKGCERCHHSGFFGRELIAECLEINEDLACAIRENQD
KTILMELAKKYGFQTMFEQGLKKAKEGLTSIDELLRVVR

Sequences:

>Translated_519_residues
MESRMDKIFQAYIDNEISLDEICTKFNITSWDFFNKLANFCNLHFVDLDEDNDFIYEGIPFSLLLKFKFLLIKNNDGFMI
IRSKPCSLELLEQVKTFMICEKIDTAIADELKIAKILNQIRIQEEIKRLSIKLRLEWQENYKRDDQSCISQIFDFLLHEI
LSFHASDIHIEARNDDALIRFRVDGILREFAIFEKDIYEALVFHVKFLACLNLAESRKTQDGSFELDFENERYDFRVSCL
PLIYGESVVIRILKHDKEILDLYKLNLGNKNLEILKKILHRPNGMILLTGPTGSGKSTTLYACLNELKSIEKKIISAEDP
IEYKIPLVQQILLNSKVGVEFNSVLRAILRQDPDIIMIGEIRDEESLDIALKASLTGHLLLSTLHTNDALSTIDRLLDMQ
AKSYLIASALSLVIAQRLVRKLCPWCKQKSKKHYIEFEGEFFEPKGCERCHHSGFFGRELIAECLEINEDLACAIRENQD
KTILMELAKKYGFQTMFEQGLKKAKEGLTSIDELLRVVR
>Mature_519_residues
MESRMDKIFQAYIDNEISLDEICTKFNITSWDFFNKLANFCNLHFVDLDEDNDFIYEGIPFSLLLKFKFLLIKNNDGFMI
IRSKPCSLELLEQVKTFMICEKIDTAIADELKIAKILNQIRIQEEIKRLSIKLRLEWQENYKRDDQSCISQIFDFLLHEI
LSFHASDIHIEARNDDALIRFRVDGILREFAIFEKDIYEALVFHVKFLACLNLAESRKTQDGSFELDFENERYDFRVSCL
PLIYGESVVIRILKHDKEILDLYKLNLGNKNLEILKKILHRPNGMILLTGPTGSGKSTTLYACLNELKSIEKKIISAEDP
IEYKIPLVQQILLNSKVGVEFNSVLRAILRQDPDIIMIGEIRDEESLDIALKASLTGHLLLSTLHTNDALSTIDRLLDMQ
AKSYLIASALSLVIAQRLVRKLCPWCKQKSKKHYIEFEGEFFEPKGCERCHHSGFFGRELIAECLEINEDLACAIRENQD
KTILMELAKKYGFQTMFEQGLKKAKEGLTSIDELLRVVR

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=408, Percent_Identity=40.6862745098039, Blast_Score=308, Evalue=6e-85,
Organism=Escherichia coli, GI1786296, Length=375, Percent_Identity=40.8, Blast_Score=291, Evalue=9e-80,
Organism=Escherichia coli, GI87082188, Length=209, Percent_Identity=38.2775119617225, Blast_Score=119, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 60146; Mature: 60146

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESRMDKIFQAYIDNEISLDEICTKFNITSWDFFNKLANFCNLHFVDLDEDNDFIYEGIP
CCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCEEEEEECCCCCEEECCCC
FSLLLKFKFLLIKNNDGFMIIRSKPCSLELLEQVKTFMICEKIDTAIADELKIAKILNQI
HHHHHEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIQEEIKRLSIKLRLEWQENYKRDDQSCISQIFDFLLHEILSFHASDIHIEARNDDALIR
HHHHHHHHEEEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEE
FRVDGILREFAIFEKDIYEALVFHVKFLACLNLAESRKTQDGSFELDFENERYDFRVSCL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEE
PLIYGESVVIRILKHDKEILDLYKLNLGNKNLEILKKILHRPNGMILLTGPTGSGKSTTL
EEECCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHH
YACLNELKSIEKKIISAEDPIEYKIPLVQQILLNSKVGVEFNSVLRAILRQDPDIIMIGE
HHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEC
IRDEESLDIALKASLTGHLLLSTLHTNDALSTIDRLLDMQAKSYLIASALSLVIAQRLVR
CCCCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLCPWCKQKSKKHYIEFEGEFFEPKGCERCHHSGFFGRELIAECLEINEDLACAIRENQD
HHCHHHHCCCCCCEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCC
KTILMELAKKYGFQTMFEQGLKKAKEGLTSIDELLRVVR
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MESRMDKIFQAYIDNEISLDEICTKFNITSWDFFNKLANFCNLHFVDLDEDNDFIYEGIP
CCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCEEEEEECCCCCEEECCCC
FSLLLKFKFLLIKNNDGFMIIRSKPCSLELLEQVKTFMICEKIDTAIADELKIAKILNQI
HHHHHEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIQEEIKRLSIKLRLEWQENYKRDDQSCISQIFDFLLHEILSFHASDIHIEARNDDALIR
HHHHHHHHEEEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEE
FRVDGILREFAIFEKDIYEALVFHVKFLACLNLAESRKTQDGSFELDFENERYDFRVSCL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEE
PLIYGESVVIRILKHDKEILDLYKLNLGNKNLEILKKILHRPNGMILLTGPTGSGKSTTL
EEECCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHH
YACLNELKSIEKKIISAEDPIEYKIPLVQQILLNSKVGVEFNSVLRAILRQDPDIIMIGE
HHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEC
IRDEESLDIALKASLTGHLLLSTLHTNDALSTIDRLLDMQAKSYLIASALSLVIAQRLVR
CCCCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLCPWCKQKSKKHYIEFEGEFFEPKGCERCHHSGFFGRELIAECLEINEDLACAIRENQD
HHCHHHHCCCCCCEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCC
KTILMELAKKYGFQTMFEQGLKKAKEGLTSIDELLRVVR
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]