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The map label for this gene is hydA [H]

Identifier: 57238436

GI number: 57238436

Start: 1487629

End: 1489122

Strand: Reverse

Name: hydA [H]

Synonym: CJE1586

Alternate gene names: 57238436

Gene position: 1489122-1487629 (Counterclockwise)

Preceding gene: 57238437

Following gene: 57238430

Centisome position: 83.76

GC content: 31.86

Gene sequence:

>1494_bases
ATGGCAAAGCTTAGCAATGAAGAATTAAAAAATATACTTGAAGATCGCATTAAAAAACTTGAGAATTCTACTTTAAAAGA
GGATAAGGTTATCAATGAAGAAAGTGTAAAAATTCTTGCTAGGCATTTATCTTTAGGCAATGAAATTCCAGCTCTTGCTC
AAAGATTTTTTCAAATCGCACCTAAAACAAAGTTAGTATGGCTTCATCTTTGTGAATGTACAGGGTGTAGCGAGAGTTTG
TTGCGTGCGGATTTACCAAGTTTTGATGAATTAGTTTTTGACTTTTTTTCTTTAGAGTATCACGAAACTTTAATGGCGGC
AAATGGGACAAAAGCTGAAGAGCTTTTAGAGTATGTTTTAGAAGAAGATTTTATTTTAGCGGTTGAAGGTGGTGTTGCAG
CCATTGATACCTTTTTTCTTACTATAGGAGCGCAAGGAGAGAGTGGCTATGAAATTTTAGAAAAACTAGCCGCTAAAGCC
AAGGCTATCTTTGCAGTGGGTACTTGTTCAAGTTATGGTGGTATTCAGGCTGCTTATCCAAATCCTAGTAAAACTTGTGG
TATTAGCGAAGTTTTAAGTCAGAAAGTAGTAAATATTCCGGGTTGTCCTCCAAGTGATATAAATATCATTGCGACTTTAA
GTTTTTTTGCTTTATTTGGAGTTTTGCCTGAACTTGATGAGCAAAATCGTCCTGTGTGGGCTTATGGTAAATGTTTGCAT
GATATATGTGAAAGAAAGGCGAAATTTGAAAGTGGGATTTTTGCTGAACATTTTGACGATGAAGCTGCAAAGAATGGAGC
TTGTTTATTTAAGATAGGTTGTAAAGGACCTTATACTTACAACAACTGTCCTAAAGTGAAATTTAATGCTAAAACTTCTT
GGCCAGTGGCTGCAGGACATGGTTGTATAGCCTGTAGTGAGAAAAATTTTTGGGATGAGTTTGGGAATTATGAAAAACCT
ATGGCAAATATTTTTTCTTACGCTAAACTTTGCAATGAAGAGTTAAAACAAGAATTTTTTCTAGAAGAGCAAATTAAAAT
TTTAGAACAAATAGATTTTGAATTTGAAAGCAATATAAAACTTATTTTACAAAATATAGCTAAAAATAAATTAGGTGCTT
CGCTTGTAGAAAATTATAAAAAATCCTTTGAGAAAAACTATGCTTTTATAGAACAAAATTTTGATGAAAATCCTATGCCT
TCAAAAGATTTTTGGAAATATCTTGAGATGAGTTTTATTTTGGTTAAAGGCGCATTTTTAAAAGATAAGAATGATTTTTT
AATTGCGGCAAAAAATTATGCTTTTAAACATGCAAGTCCTTATGATTTTAAGTTAAATATGAATGCTGAAAAACCAAAGC
TTGATGTGAGTAAATCTTTTCGTATGACTTTAATCTATCTTTGCGGTGGGCTTGATTTTGAAGGTATTGCTTATAGTATT
TTAAAAGCTTTTGAAGATAATATTACTAAAATTTCATCATTAAAAGCAAGTTAA

Upstream 100 bases:

>100_bases
TTATGTTGATGCAGGATATAACATCATGGGTATGGGCGATGTTGAAAAAAATGAAGAAGGGCAAACTGTTCTTTGTTGGG
ATAATCAAAAAGGATAAAAA

Downstream 100 bases:

>100_bases
TTTAATAAAATTTTAGCAATATGAAGCCCTATAAAAGCTACTATATAAGCACTTGTGCAGGTAAAAAGAAAGAGAAAGAG
AGTATATTTTAGCTTTCCAC

Product: hydrogenase, (NiFe)/(NiFeSe) small subunit family

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MAKLSNEELKNILEDRIKKLENSTLKEDKVINEESVKILARHLSLGNEIPALAQRFFQIAPKTKLVWLHLCECTGCSESL
LRADLPSFDELVFDFFSLEYHETLMAANGTKAEELLEYVLEEDFILAVEGGVAAIDTFFLTIGAQGESGYEILEKLAAKA
KAIFAVGTCSSYGGIQAAYPNPSKTCGISEVLSQKVVNIPGCPPSDINIIATLSFFALFGVLPELDEQNRPVWAYGKCLH
DICERKAKFESGIFAEHFDDEAAKNGACLFKIGCKGPYTYNNCPKVKFNAKTSWPVAAGHGCIACSEKNFWDEFGNYEKP
MANIFSYAKLCNEELKQEFFLEEQIKILEQIDFEFESNIKLILQNIAKNKLGASLVENYKKSFEKNYAFIEQNFDENPMP
SKDFWKYLEMSFILVKGAFLKDKNDFLIAAKNYAFKHASPYDFKLNMNAEKPKLDVSKSFRMTLIYLCGGLDFEGIAYSI
LKAFEDNITKISSLKAS

Sequences:

>Translated_497_residues
MAKLSNEELKNILEDRIKKLENSTLKEDKVINEESVKILARHLSLGNEIPALAQRFFQIAPKTKLVWLHLCECTGCSESL
LRADLPSFDELVFDFFSLEYHETLMAANGTKAEELLEYVLEEDFILAVEGGVAAIDTFFLTIGAQGESGYEILEKLAAKA
KAIFAVGTCSSYGGIQAAYPNPSKTCGISEVLSQKVVNIPGCPPSDINIIATLSFFALFGVLPELDEQNRPVWAYGKCLH
DICERKAKFESGIFAEHFDDEAAKNGACLFKIGCKGPYTYNNCPKVKFNAKTSWPVAAGHGCIACSEKNFWDEFGNYEKP
MANIFSYAKLCNEELKQEFFLEEQIKILEQIDFEFESNIKLILQNIAKNKLGASLVENYKKSFEKNYAFIEQNFDENPMP
SKDFWKYLEMSFILVKGAFLKDKNDFLIAAKNYAFKHASPYDFKLNMNAEKPKLDVSKSFRMTLIYLCGGLDFEGIAYSI
LKAFEDNITKISSLKAS
>Mature_496_residues
AKLSNEELKNILEDRIKKLENSTLKEDKVINEESVKILARHLSLGNEIPALAQRFFQIAPKTKLVWLHLCECTGCSESLL
RADLPSFDELVFDFFSLEYHETLMAANGTKAEELLEYVLEEDFILAVEGGVAAIDTFFLTIGAQGESGYEILEKLAAKAK
AIFAVGTCSSYGGIQAAYPNPSKTCGISEVLSQKVVNIPGCPPSDINIIATLSFFALFGVLPELDEQNRPVWAYGKCLHD
ICERKAKFESGIFAEHFDDEAAKNGACLFKIGCKGPYTYNNCPKVKFNAKTSWPVAAGHGCIACSEKNFWDEFGNYEKPM
ANIFSYAKLCNEELKQEFFLEEQIKILEQIDFEFESNIKLILQNIAKNKLGASLVENYKKSFEKNYAFIEQNFDENPMPS
KDFWKYLEMSFILVKGAFLKDKNDFLIAAKNYAFKHASPYDFKLNMNAEKPKLDVSKSFRMTLIYLCGGLDFEGIAYSIL
KAFEDNITKISSLKAS

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=286, Percent_Identity=39.1608391608392, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1789371, Length=268, Percent_Identity=41.7910447761194, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 55920; Mature: 55788

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLSNEELKNILEDRIKKLENSTLKEDKVINEESVKILARHLSLGNEIPALAQRFFQIA
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHC
PKTKLVWLHLCECTGCSESLLRADLPSFDELVFDFFSLEYHETLMAANGTKAEELLEYVL
CCCEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EEDFILAVEGGVAAIDTFFLTIGAQGESGYEILEKLAAKAKAIFAVGTCSSYGGIQAAYP
CCCEEEEECCCHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCCCCCCEEECCC
NPSKTCGISEVLSQKVVNIPGCPPSDINIIATLSFFALFGVLPELDEQNRPVWAYGKCLH
CCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHH
DICERKAKFESGIFAEHFDDEAAKNGACLFKIGCKGPYTYNNCPKVKFNAKTSWPVAAGH
HHHHHHHHHHCCCHHHHCCHHHCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCEECCC
GCIACSEKNFWDEFGNYEKPMANIFSYAKLCNEELKQEFFLEEQIKILEQIDFEFESNIK
CEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LILQNIAKNKLGASLVENYKKSFEKNYAFIEQNFDENPMPSKDFWKYLEMSFILVKGAFL
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHEEECHHE
KDKNDFLIAAKNYAFKHASPYDFKLNMNAEKPKLDVSKSFRMTLIYLCGGLDFEGIAYSI
ECCCCEEEEECCCEECCCCCCEEEEECCCCCCCCCCCHHHHEEEEEEECCCCHHHHHHHH
LKAFEDNITKISSLKAS
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKLSNEELKNILEDRIKKLENSTLKEDKVINEESVKILARHLSLGNEIPALAQRFFQIA
CCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHC
PKTKLVWLHLCECTGCSESLLRADLPSFDELVFDFFSLEYHETLMAANGTKAEELLEYVL
CCCEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EEDFILAVEGGVAAIDTFFLTIGAQGESGYEILEKLAAKAKAIFAVGTCSSYGGIQAAYP
CCCEEEEECCCHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCCCCCCEEECCC
NPSKTCGISEVLSQKVVNIPGCPPSDINIIATLSFFALFGVLPELDEQNRPVWAYGKCLH
CCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHH
DICERKAKFESGIFAEHFDDEAAKNGACLFKIGCKGPYTYNNCPKVKFNAKTSWPVAAGH
HHHHHHHHHHCCCHHHHCCHHHCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCEECCC
GCIACSEKNFWDEFGNYEKPMANIFSYAKLCNEELKQEFFLEEQIKILEQIDFEFESNIK
CEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LILQNIAKNKLGASLVENYKKSFEKNYAFIEQNFDENPMPSKDFWKYLEMSFILVKGAFL
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHEEECHHE
KDKNDFLIAAKNYAFKHASPYDFKLNMNAEKPKLDVSKSFRMTLIYLCGGLDFEGIAYSI
ECCCCEEEEECCCEECCCCCCEEEEECCCCCCCCCCCHHHHEEEEEEECCCCHHHHHHHH
LKAFEDNITKISSLKAS
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]