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The map label for this gene is fabI [H]

Identifier: 57238437

GI number: 57238437

Start: 1489126

End: 1489950

Strand: Reverse

Name: fabI [H]

Synonym: CJE1587

Alternate gene names: 57238437

Gene position: 1489950-1489126 (Counterclockwise)

Preceding gene: 57238438

Following gene: 57238436

Centisome position: 83.81

GC content: 33.09

Gene sequence:

>825_bases
ATGATTATGAAGGGTAAAAAAGGCCTTATTGTAGGGGTTGCGAATAATAAATCTATCGCTTATGGCATAGCAAAAGCATG
TTTTGATCAAGGAGCCGAGCTTGCTTTTACTTTTTTAAATGATGCTTTAAAAAAACGCGTAGAACCTATAGCACAAGAGT
TTAATTCTAATTTTGTTTATGAGCTTGATGTAAATAATAACGAGCATTTAGATTCTATCGCTGAAAAAATCAAAAAAGAT
TTAGGCGAGATTGATTTTGTAGTGCATGCGGTTGCTTTTGCTCCAAAAGAAGCTTTAGAAAATTCTTTTTTAGAAACTTC
AAAAGAAGCTTTTGATATAGCTATGCAAACTTCTGTGTATTCTTTACTTTCTTTAACACGTTCGCTTTTACCTATTTTAA
AAGATAAGGGTTCGATTTTGACTTTAAGTTATCTTGGTGGGGTTAAATATGTACCTCATTATAATGTTATGGGTGTAGCA
AAAGCTGCACTTGAAAGTTCTGTGCGTTATTTGGCAAGAGATTTAGGGGTAAAAGGAATTCGTGTTAATGCTATTTCAGC
AGGTCCTATTAAAACTTTGGCAGCAAGTGGAATAGGGGATTTTAGAATGATTTTAAAATACAATGAAATCAATTCTCCTT
TAAAACGCAATGTTAGTATAGAAGATGTAGGCAATTCGGCTATGTATTTACTTAGTGATTTAGCAAATGGTGTTACAGGT
GAAATACATTATGTTGATGCAGGATATAACATCATGGGTATGGGCGATGTTGAAAAAAATGAAGAAGGGCAAACTGTTCT
TTGTTGGGATAATCAAAAAGGATAA

Upstream 100 bases:

>100_bases
TATCAAAGAAATTTTAAGTGTAAATCATTGTGGTGGAGTTTTGATAGGCTCTGCAGCTTTAAAAGTAGAAAATTTTATAA
AATTAATCAAAGGATAAAAG

Downstream 100 bases:

>100_bases
AAAATGGCAAAGCTTAGCAATGAAGAATTAAAAAATATACTTGAAGATCGCATTAAAAAACTTGAGAATTCTACTTTAAA
AGAGGATAAGGTTATCAATG

Product: enoyl-ACP reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MIMKGKKGLIVGVANNKSIAYGIAKACFDQGAELAFTFLNDALKKRVEPIAQEFNSNFVYELDVNNNEHLDSIAEKIKKD
LGEIDFVVHAVAFAPKEALENSFLETSKEAFDIAMQTSVYSLLSLTRSLLPILKDKGSILTLSYLGGVKYVPHYNVMGVA
KAALESSVRYLARDLGVKGIRVNAISAGPIKTLAASGIGDFRMILKYNEINSPLKRNVSIEDVGNSAMYLLSDLANGVTG
EIHYVDAGYNIMGMGDVEKNEEGQTVLCWDNQKG

Sequences:

>Translated_274_residues
MIMKGKKGLIVGVANNKSIAYGIAKACFDQGAELAFTFLNDALKKRVEPIAQEFNSNFVYELDVNNNEHLDSIAEKIKKD
LGEIDFVVHAVAFAPKEALENSFLETSKEAFDIAMQTSVYSLLSLTRSLLPILKDKGSILTLSYLGGVKYVPHYNVMGVA
KAALESSVRYLARDLGVKGIRVNAISAGPIKTLAASGIGDFRMILKYNEINSPLKRNVSIEDVGNSAMYLLSDLANGVTG
EIHYVDAGYNIMGMGDVEKNEEGQTVLCWDNQKG
>Mature_274_residues
MIMKGKKGLIVGVANNKSIAYGIAKACFDQGAELAFTFLNDALKKRVEPIAQEFNSNFVYELDVNNNEHLDSIAEKIKKD
LGEIDFVVHAVAFAPKEALENSFLETSKEAFDIAMQTSVYSLLSLTRSLLPILKDKGSILTLSYLGGVKYVPHYNVMGVA
KAALESSVRYLARDLGVKGIRVNAISAGPIKTLAASGIGDFRMILKYNEINSPLKRNVSIEDVGNSAMYLLSDLANGVTG
EIHYVDAGYNIMGMGDVEKNEEGQTVLCWDNQKG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=46.5116279069767, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=27.843137254902, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=28.1746031746032, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=24.8062015503876, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367365, Length=250, Percent_Identity=27.2, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787335, Length=251, Percent_Identity=23.9043824701195, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=203, Percent_Identity=25.1231527093596, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=200, Percent_Identity=25, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=209, Percent_Identity=31.1004784688995, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21357041, Length=195, Percent_Identity=31.2820512820513, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI23397609, Length=239, Percent_Identity=25.9414225941423, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571526, Length=177, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24644337, Length=195, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24643142, Length=156, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24644339, Length=259, Percent_Identity=26.2548262548263, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29867; Mature: 29867

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKGKKGLIVGVANNKSIAYGIAKACFDQGAELAFTFLNDALKKRVEPIAQEFNSNFVY
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
ELDVNNNEHLDSIAEKIKKDLGEIDFVVHAVAFAPKEALENSFLETSKEAFDIAMQTSVY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSLTRSLLPILKDKGSILTLSYLGGVKYVPHYNVMGVAKAALESSVRYLARDLGVKGI
HHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
RVNAISAGPIKTLAASGIGDFRMILKYNEINSPLKRNVSIEDVGNSAMYLLSDLANGVTG
EEEEECCCCHHHHHHCCCCCEEEEEEECCCCCHHHHCCCHHHCCCHHHHHHHHHCCCCCC
EIHYVDAGYNIMGMGDVEKNEEGQTVLCWDNQKG
EEEEEECCCEEEECCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MIMKGKKGLIVGVANNKSIAYGIAKACFDQGAELAFTFLNDALKKRVEPIAQEFNSNFVY
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
ELDVNNNEHLDSIAEKIKKDLGEIDFVVHAVAFAPKEALENSFLETSKEAFDIAMQTSVY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSLTRSLLPILKDKGSILTLSYLGGVKYVPHYNVMGVAKAALESSVRYLARDLGVKGI
HHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
RVNAISAGPIKTLAASGIGDFRMILKYNEINSPLKRNVSIEDVGNSAMYLLSDLANGVTG
EEEEECCCCHHHHHHCCCCCEEEEEEECCCCCHHHHCCCHHHCCCHHHHHHHHHCCCCCC
EIHYVDAGYNIMGMGDVEKNEEGQTVLCWDNQKG
EEEEEECCCEEEECCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]