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The map label for this gene is gltX [H]

Identifier: 57238338

GI number: 57238338

Start: 1376799

End: 1378190

Strand: Reverse

Name: gltX [H]

Synonym: CJE1480

Alternate gene names: 57238338

Gene position: 1378190-1376799 (Counterclockwise)

Preceding gene: 57238340

Following gene: 57238337

Centisome position: 77.52

GC content: 33.48

Gene sequence:

>1392_bases
ATGCACGAAAAACTTACAACGCGTTTTGCTCCAAGCCCAACAGGGTATTTGCACATAGGTGGACTTAGAACAGCTCTTTA
TAATTATTTATACGCTAGAAAAAATGGTGGAAATTTTTTACTTCGTATCGAAGATACGGATTTAAAAAGAAATTCCAAAG
AAGCTACTAAAGCAATTATCGAAGCTTTTAAATGGTGCGGTTTAGAACATGATGGAGAAGTAACTTACCAATCAGAGCGT
TTTGATCTTTATAAAGAATATGTTAAAAAGCTTTTAGATGAAGGCAAGGCGTATTATTGTTATATGAGTAAAGAAGAATT
AGAAGAATTGCGCGCCAAGCAAGAAGCTGCCAAAGAGCGCCCAAGATATGATGGAAGATATAGAGAATTTACAGGCACGC
CACCTCAGGGTATAGAGCCTGTTGTGCGTATTAAAGCACCTCAAAGCGGTGAGATTGTTTTTAAAGATGGGGTTAAAGGC
GAGGTGAGATTTAAAGCAGAGGATATAATGGATGATTTTATCATTGCAAGAAGTGATGGAACTCCAACTTATAATTTTAC
TGTTGTTATTGATGATGCTTTAATGGGGGTAAGTGATGTGATTCGCGGAGATGATCATCTTTCAAATACTCCTAAACAAA
TTGTGCTTTATGAGGCATTGGGTTTTAAAATTCCTAAATTTTTTCATGTGGCTATGATACATGGAGAAGATGGTAAAAAG
CTTTCAAAAAGACATGGGGCAACTGATGTTATGGAATACAAAGAAATGGGAATTTTACCTCAAGCTTTGCTTAATTTTCT
TGTGCGTTTAGGTTGGAGCCATGGTGATGATGAGGTTTTTTCATTAGAAGATTTGAAAAAACTTTTTGACCCTTATCATA
TTAATAAAAGCGCATCTTGTTATAATGCTAAAAAGCTTGAATGGCTTAACGCTCATTATATTAAAACTTTGCCTTTTGAA
GAGATTAACCGTCAGCTTAAGGATCTTGGCTTTGATTTATCTGTTTATGAAAAGGCAGGATTTTTACTTGATTTATTAAG
AGAGCGTGCAAAAACCTTGCATGATATTATCAATGGTGCAAAAAGTATAGTTAATGCGCCGCAAAATTATGATGAAAATG
CAGTACAAAAATTTATTAACAAAAATAATTTAGAATTGCTCCAAGCATTTGCTAATACACTTAAAGATCAAAAAACAGGT
AAAGATTTTGAAGATTTTACAAATGATTTTTTAGAAAAAAAAGAAGCAAAGTTAAAAGATTTGGCTCAGCCTATACGCAT
AGCTTTAACAGGGAGTGCGGTAAGTCCTAGTATTTTTGAAGTATTAGAATTTTTAGAGGTTGATGAGTGCAAAAAAAGAA
TAGAAAATTTTTTAAAAGTTAGAGGAAAATAA

Upstream 100 bases:

>100_bases
TGCAAAAAATGCAAAACTTAATAAAATTTTTTTCATTTCAAACCTTTATTTAAGTATAAATATCGCATTATATCAATAAA
AATTTAAGAAAAGGCAAAAA

Downstream 100 bases:

>100_bases
AATGAATTTAAAAGAAGAAGCTTTAAAATACCATTTAGGTGGGAAAATTGATATTGTACCAAGTAAGCCAATGGCTACAA
GCCATGATCTATCTTTGGCG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAIIEAFKWCGLEHDGEVTYQSER
FDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKERPRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKG
EVRFKAEDIMDDFIIARSDGTPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPKFFHVAMIHGEDGKK
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASCYNAKKLEWLNAHYIKTLPFE
EINRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGAKSIVNAPQNYDENAVQKFINKNNLELLQAFANTLKDQKTG
KDFEDFTNDFLEKKEAKLKDLAQPIRIALTGSAVSPSIFEVLEFLEVDECKKRIENFLKVRGK

Sequences:

>Translated_463_residues
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAIIEAFKWCGLEHDGEVTYQSER
FDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKERPRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKG
EVRFKAEDIMDDFIIARSDGTPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPKFFHVAMIHGEDGKK
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASCYNAKKLEWLNAHYIKTLPFE
EINRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGAKSIVNAPQNYDENAVQKFINKNNLELLQAFANTLKDQKTG
KDFEDFTNDFLEKKEAKLKDLAQPIRIALTGSAVSPSIFEVLEFLEVDECKKRIENFLKVRGK
>Mature_463_residues
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAIIEAFKWCGLEHDGEVTYQSER
FDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKERPRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKG
EVRFKAEDIMDDFIIARSDGTPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPKFFHVAMIHGEDGKK
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASCYNAKKLEWLNAHYIKTLPFE
EINRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGAKSIVNAPQNYDENAVQKFINKNNLELLQAFANTLKDQKTG
KDFEDFTNDFLEKKEAKLKDLAQPIRIALTGSAVSPSIFEVLEFLEVDECKKRIENFLKVRGK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=29.9794661190965, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI62241042, Length=259, Percent_Identity=25.8687258687259, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4826960, Length=293, Percent_Identity=23.2081911262799, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788743, Length=442, Percent_Identity=44.5701357466063, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI226510925, Length=278, Percent_Identity=35.2517985611511, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1786895, Length=207, Percent_Identity=29.4685990338164, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71993394, Length=491, Percent_Identity=32.5865580448065, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17511007, Length=236, Percent_Identity=25.4237288135593, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=118, Percent_Identity=32.2033898305085, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=35.1020408163265, Blast_Score=262, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6321192, Length=245, Percent_Identity=27.7551020408163, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=307, Percent_Identity=27.0358306188925, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24665321, Length=476, Percent_Identity=32.3529411764706, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI17864488, Length=230, Percent_Identity=27.3913043478261, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53158; Mature: 53158

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAII
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHHH
EAFKWCGLEHDGEVTYQSERFDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKER
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHC
PRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKGEVRFKAEDIMDDFIIARSDG
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEHHHHHHHHEEEECCC
TPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPKFFHVAMIHGEDGKK
CCCEEEEEEECCHHHHHHHHHCCCCCCCCCCHHEEEEHHHCCCCCCEEEEEEEECCCCHH
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASC
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECHHHHHHHHCCCCCCCCCCC
YNAKKLEWLNAHYIKTLPFEEINRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGA
CCCHHHHHHCCHHHEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSIVNAPQNYDENAVQKFINKNNLELLQAFANTLKDQKTGKDFEDFTNDFLEKKEAKLKD
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LAQPIRIALTGSAVSPSIFEVLEFLEVDECKKRIENFLKVRGK
HHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAII
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHHH
EAFKWCGLEHDGEVTYQSERFDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKER
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHC
PRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKGEVRFKAEDIMDDFIIARSDG
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEHHHHHHHHEEEECCC
TPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPKFFHVAMIHGEDGKK
CCCEEEEEEECCHHHHHHHHHCCCCCCCCCCHHEEEEHHHCCCCCCEEEEEEEECCCCHH
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASC
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECHHHHHHHHCCCCCCCCCCC
YNAKKLEWLNAHYIKTLPFEEINRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGA
CCCHHHHHHCCHHHEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSIVNAPQNYDENAVQKFINKNNLELLQAFANTLKDQKTGKDFEDFTNDFLEKKEAKLKD
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LAQPIRIALTGSAVSPSIFEVLEFLEVDECKKRIENFLKVRGK
HHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA