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The map label for this gene is maeB [H]

Identifier: 57238337

GI number: 57238337

Start: 1375562

End: 1376797

Strand: Reverse

Name: maeB [H]

Synonym: CJE1479

Alternate gene names: 57238337

Gene position: 1376797-1375562 (Counterclockwise)

Preceding gene: 57238338

Following gene: 57238336

Centisome position: 77.44

GC content: 35.6

Gene sequence:

>1236_bases
ATGAATTTAAAAGAAGAAGCTTTAAAATACCATTTAGGTGGGAAAATTGATATTGTACCAAGTAAGCCAATGGCTACAAG
CCATGATCTATCTTTGGCGTATAGCCCAGGAGTTGCAGAACCTTGCCTTGAAATCGCTAAAGATAATGAGCTTGCTTATA
CTTATACAAATAAGGCTAATTTAGTAGCTATAGTTAGTGATGGTTCTGCTGTTTTAGGACTTGGAAACATAGGAGCGCAG
GCTTCAAAACCTGTTATGGAAGGTAAGGCTTGTTTGTTTAAAAAATTTGCAAATGTCAATGCTTATGATATAGAAATTGA
TGTTCATAGTGTGGAAGAGATTGTAAATTTTTGCAAAGCTTTGGCTCCAACTGTGGGCGGGATAAATTTAGAAGATATAG
CTGCTCCAAAATGTTTTGAAATCGAAGCGGCTTTGCAAGATTTAGGAATTCCTGTAATGCACGATGATCAGCACGGAACA
GCTATTATTTCAACTGCGGGACTTATGAATGCTATGGAAATAAGTGGTAAAAAATTTAAAGACATCAAAGTAGTTGTAAG
TGGTGCAGGTGCCGCAGGTATAGCAAGTGCTAAAATGTATAGAAATTTAGGGGTCGAAAATATTATTTTAGTAGATAGCA
AAGGAGTGATTTCTAAAGATAGAAATGATTTAACTCCTCAAAAGCTTGAATTTGCTGTTGATTCTAAGGAAAAGACTTTA
AAAGAAGTTTTAAAAGGAGCTGATGTTTTCCTAGGACTTAGTGCACCAAAAATTCTTGATGATGAAATGGTTTTAAGCAT
GGCAAAAGATCCTGTGATTTTTGCTTTGGCAAATCCTATTCCTGAAGTGATGCCTGAAGATGTAGCTAGACTTAGAAAAG
ATGCGATTGTAGGAACAGGAAGAAGTGATTATCCAAATCAAATCAATAATGTTTTAGGTTTTCCTTTTATTTTCCGTGGG
GCTTTAGATGTGCGTGCAAGTAAAATCACTGAAAATATGAAAGTAGCGGCTGCTAAAGCATTAGCAGATCTTGCAAAACT
TCCTGTAAGCGATGCGGTTAAAAAAGCTTATAATTTAAGCACTTTAGAATTTGGAAGAGATTATGTTATACCAAAACCTT
TTGATGAAAGAGTAAAAGCTGCAGTAAGTACAGCTGTAGCAGCAGCTGCGGTAAAAGATGGTGTGGCTAAGGTGAAAAAT
TTTGATGAGAAAGCTTATTTTGAAAGCTTAAAATGA

Upstream 100 bases:

>100_bases
AGTGCGGTAAGTCCTAGTATTTTTGAAGTATTAGAATTTTTAGAGGTTGATGAGTGCAAAAAAAGAATAGAAAATTTTTT
AAAAGTTAGAGGAAAATAAA

Downstream 100 bases:

>100_bases
AAAATATTCATTGTATCAATCATCCATTGATCGAACATAAATTAGGAATTTTAAGGGCTAAAGAGACAAAGCCCTTTCAA
TTTCGTATGTTAATCGATGA

Product: NADP-dependent malic enzyme, truncation

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKANLVAIVSDGSAVLGLGNIGAQ
ASKPVMEGKACLFKKFANVNAYDIEIDVHSVEEIVNFCKALAPTVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGT
AIISTAGLMNAMEISGKKFKDIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTPQKLEFAVDSKEKTL
KEVLKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTGRSDYPNQINNVLGFPFIFRG
ALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLSTLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKN
FDEKAYFESLK

Sequences:

>Translated_411_residues
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKANLVAIVSDGSAVLGLGNIGAQ
ASKPVMEGKACLFKKFANVNAYDIEIDVHSVEEIVNFCKALAPTVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGT
AIISTAGLMNAMEISGKKFKDIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTPQKLEFAVDSKEKTL
KEVLKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTGRSDYPNQINNVLGFPFIFRG
ALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLSTLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKN
FDEKAYFESLK
>Mature_411_residues
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKANLVAIVSDGSAVLGLGNIGAQ
ASKPVMEGKACLFKKFANVNAYDIEIDVHSVEEIVNFCKALAPTVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGT
AIISTAGLMNAMEISGKKFKDIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTPQKLEFAVDSKEKTL
KEVLKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTGRSDYPNQINNVLGFPFIFRG
ALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLSTLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKN
FDEKAYFESLK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=344, Percent_Identity=26.453488372093, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI62420882, Length=395, Percent_Identity=24.5569620253165, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI62420880, Length=395, Percent_Identity=24.5569620253165, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239049447, Length=395, Percent_Identity=24.5569620253165, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788806, Length=417, Percent_Identity=53.7170263788969, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI87081919, Length=454, Percent_Identity=24.0088105726872, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646388, Length=344, Percent_Identity=28.1976744186047, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24646386, Length=344, Percent_Identity=28.1976744186047, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI78707242, Length=393, Percent_Identity=24.6819338422392, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI78707238, Length=393, Percent_Identity=24.6819338422392, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI78707240, Length=390, Percent_Identity=24.3589743589744, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281362674, Length=398, Percent_Identity=25.6281407035176, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281362672, Length=397, Percent_Identity=25.1889168765743, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356279, Length=398, Percent_Identity=25.6281407035176, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 43940; Mature: 43940

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKAN
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCC
LVAIVSDGSAVLGLGNIGAQASKPVMEGKACLFKKFANVNAYDIEIDVHSVEEIVNFCKA
EEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHH
LAPTVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGTAIISTAGLMNAMEISGKKFK
HCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCC
DIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTPQKLEFAVDSKEKTL
EEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHEEECCHHHHH
KEVLKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTG
HHHHCCCCEEEECCCCCHHCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHEEECC
RSDYPNQINNVLGFPFIFRGALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLS
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
TLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKNFDEKAYFESLK
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKAN
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCC
LVAIVSDGSAVLGLGNIGAQASKPVMEGKACLFKKFANVNAYDIEIDVHSVEEIVNFCKA
EEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHH
LAPTVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGTAIISTAGLMNAMEISGKKFK
HCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCC
DIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTPQKLEFAVDSKEKTL
EEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHEEECCHHHHH
KEVLKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTG
HHHHCCCCEEEECCCCCHHCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHEEECC
RSDYPNQINNVLGFPFIFRGALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLS
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
TLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKNFDEKAYFESLK
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]