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The map label for this gene is mdh

Identifier: 57238268

GI number: 57238268

Start: 574719

End: 575621

Strand: Direct

Name: mdh

Synonym: CJE0636

Alternate gene names: 57238268

Gene position: 574719-575621 (Clockwise)

Preceding gene: 57238269

Following gene: 57238267

Centisome position: 32.33

GC content: 30.9

Gene sequence:

>903_bases
ATGAAAATCACTGTTATAGGGGCTGGCAATGTAGGTTCAAGCGTAGCTTATGCTTTGATTTTAAGAGAAATAGCAAATGA
AATTGTTTTAGTAGATATTAACGAGGATTTGCTTTACGCAAAAGAACTAGAGCTTACTCAAAGCATAGCTGCTTTAAATT
TAAATATAGATTTACTTTGCACTAAAGATTATACTCATACCAAAAATTCAGATATAGTGCTTTTTAGTGCAGGTTTTGCT
AGAAAAGATGGGCAAAGTAGAGAAGAATTGCTTCAGTTAAATACAAGCATTATGCTTGATTGTGCTAAAAAAATTAAAGA
TTTTACAGAAGATCCGCTTTTTATTATTTTAACTAATCCTGTGGATTTTCTACTTAATACTCTTTATGAAAGCGGGATTT
TTTCATCAAAGAAAATTATAGCCATGGCGGGTGTTTTAGACAATGCAAGATTTAAGTATGAACTTGCCAAAAAACTTAAT
GTCAAAATGTCAAGAGTGGATACAAGACTTATTGGTTTTCATAATGATGATATGGTTTTGGTAAAATCTTACGCAAGTGT
TAAAAATAAAAATATAAGCGAATTTTTAAATGAAGAAGAATTTGAGGATTTAGAAAATGAAGTTAAAACAGGTGGAGCTA
AAGTAATCAAACATCTTAAAACTTCAGCTTATTTAGCCCCTGCTAGTGCTTGTATAAGAATGCTTGAGTCTATAAGAAGT
GGCGAGTTTTTACCTATGAGTGTGATTTTACATGGAGAATTTGGAGTGCAAAACAAAGCCTTGGGAGTTATGGCAAGATT
AGGACTTGAAGGTGTGATTGAAATCATGAAAATGGATCTTAGTTTGCAAGAAAAAGACAAATTAGAAAAATCTTTAATCA
AATATCAATATAAAGGAGAATAA

Upstream 100 bases:

>100_bases
TGGATTTGGGTGGGTATTATAAATTTGATGATGAAAAAGCAAATAAAATTATGCGTCCAAGTGCAACTTTTAACGCTATT
TTAGAAAAAATAGGACAAAG

Downstream 100 bases:

>100_bases
ATGAACATACATGAGTATCAAGCAAAAGCAATTTTTGTAGACAATGGCATCCCTACCTTAAAGGGAAAAGTTGCATTTAG
CGTAGATGAAGCTGTGGCTA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKITVIGAGNVGSSVAYALILREIANEIVLVDINEDLLYAKELELTQSIAALNLNIDLLCTKDYTHTKNSDIVLFSAGFA
RKDGQSREELLQLNTSIMLDCAKKIKDFTEDPLFIILTNPVDFLLNTLYESGIFSSKKIIAMAGVLDNARFKYELAKKLN
VKMSRVDTRLIGFHNDDMVLVKSYASVKNKNISEFLNEEEFEDLENEVKTGGAKVIKHLKTSAYLAPASACIRMLESIRS
GEFLPMSVILHGEFGVQNKALGVMARLGLEGVIEIMKMDLSLQEKDKLEKSLIKYQYKGE

Sequences:

>Translated_300_residues
MKITVIGAGNVGSSVAYALILREIANEIVLVDINEDLLYAKELELTQSIAALNLNIDLLCTKDYTHTKNSDIVLFSAGFA
RKDGQSREELLQLNTSIMLDCAKKIKDFTEDPLFIILTNPVDFLLNTLYESGIFSSKKIIAMAGVLDNARFKYELAKKLN
VKMSRVDTRLIGFHNDDMVLVKSYASVKNKNISEFLNEEEFEDLENEVKTGGAKVIKHLKTSAYLAPASACIRMLESIRS
GEFLPMSVILHGEFGVQNKALGVMARLGLEGVIEIMKMDLSLQEKDKLEKSLIKYQYKGE
>Mature_300_residues
MKITVIGAGNVGSSVAYALILREIANEIVLVDINEDLLYAKELELTQSIAALNLNIDLLCTKDYTHTKNSDIVLFSAGFA
RKDGQSREELLQLNTSIMLDCAKKIKDFTEDPLFIILTNPVDFLLNTLYESGIFSSKKIIAMAGVLDNARFKYELAKKLN
VKMSRVDTRLIGFHNDDMVLVKSYASVKNKNISEFLNEEEFEDLENEVKTGGAKVIKHLKTSAYLAPASACIRMLESIRS
GEFLPMSVILHGEFGVQNKALGVMARLGLEGVIEIMKMDLSLQEKDKLEKSLIKYQYKGE

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=301, Percent_Identity=27.5747508305648, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI221136809, Length=301, Percent_Identity=27.5747508305648, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI15082234, Length=301, Percent_Identity=27.5747508305648, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI260099723, Length=300, Percent_Identity=29, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI5031857, Length=300, Percent_Identity=29, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI291575128, Length=300, Percent_Identity=28.6666666666667, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4557032, Length=300, Percent_Identity=28.6666666666667, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI9257228, Length=300, Percent_Identity=27.6666666666667, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4504973, Length=300, Percent_Identity=27.6666666666667, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI260099727, Length=186, Percent_Identity=31.1827956989247, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI260099725, Length=186, Percent_Identity=31.1827956989247, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI103472011, Length=183, Percent_Identity=24.0437158469945, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789632, Length=320, Percent_Identity=25.3125, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17535107, Length=289, Percent_Identity=25.2595155709343, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136226, Length=301, Percent_Identity=25.9136212624585, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45550422, Length=301, Percent_Identity=27.2425249169435, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24647881, Length=250, Percent_Identity=27.2, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_CAMJE (Q9PHY2)

Other databases:

- EMBL:   AL111168
- PIR:   D81399
- RefSeq:   YP_002343963.1
- ProteinModelPortal:   Q9PHY2
- SMR:   Q9PHY2
- IntAct:   Q9PHY2
- GeneID:   904860
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj0532
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- ProtClustDB:   CLSK878824
- BioCyc:   CJEJ192222:CJ0532-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33513; Mature: 33513

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: ACT_SITE 174-174 BINDING 32-32 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVIGAGNVGSSVAYALILREIANEIVLVDINEDLLYAKELELTQSIAALNLNIDLLC
CEEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHEEECCEEEEE
TKDYTHTKNSDIVLFSAGFARKDGQSREELLQLNTSIMLDCAKKIKDFTEDPLFIILTNP
ECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
VDFLLNTLYESGIFSSKKIIAMAGVLDNARFKYELAKKLNVKMSRVDTRLIGFHNDDMVL
HHHHHHHHHHHCCCCCCHHEEEECHHCCCHHHHHHHHHHCCHHHHHCEEEEEECCCCEEE
VKSYASVKNKNISEFLNEEEFEDLENEVKTGGAKVIKHLKTSAYLAPASACIRMLESIRS
EEHHHHHCCCCHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GEFLPMSVILHGEFGVQNKALGVMARLGLEGVIEIMKMDLSLQEKDKLEKSLIKYQYKGE
CCCEEEEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MKITVIGAGNVGSSVAYALILREIANEIVLVDINEDLLYAKELELTQSIAALNLNIDLLC
CEEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHEEECCEEEEE
TKDYTHTKNSDIVLFSAGFARKDGQSREELLQLNTSIMLDCAKKIKDFTEDPLFIILTNP
ECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
VDFLLNTLYESGIFSSKKIIAMAGVLDNARFKYELAKKLNVKMSRVDTRLIGFHNDDMVL
HHHHHHHHHHHCCCCCCHHEEEECHHCCCHHHHHHHHHHCCHHHHHCEEEEEECCCCEEE
VKSYASVKNKNISEFLNEEEFEDLENEVKTGGAKVIKHLKTSAYLAPASACIRMLESIRS
EEHHHHHCCCCHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GEFLPMSVILHGEFGVQNKALGVMARLGLEGVIEIMKMDLSLQEKDKLEKSLIKYQYKGE
CCCEEEEEEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204