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The map label for this gene is ribB

Identifier: 57238232

GI number: 57238232

Start: 609716

End: 610735

Strand: Direct

Name: ribB

Synonym: CJE0675

Alternate gene names: 57238232

Gene position: 609716-610735 (Clockwise)

Preceding gene: 57238233

Following gene: 57238231

Centisome position: 34.3

GC content: 32.16

Gene sequence:

>1020_bases
ATGAAATTTGTTAGCGTAGAACAAGCGATTAAGGATTTACAAGCAGGAAAAATGCTTGTTATGGTAGATGCAGAAGATAG
AGAAAATGAAGGCGATTTAATCTTTCCTGCGCAATTTAGCACTCAAGAAAAAGTAAATTTCATGATAAAAGAAGCTCGTG
GTGTGGTTTGTGTAGCCTTAGATGAAACTTTGGCAAAAAAATTTGAACTTCCTTTAATGGTGCCTAAAAATACTTCAAAC
CACGAAACTGCTTTTACTATAACCGTAGATGCAAAAGATGCTACAACGGGAGTTAGCGCCTATGAAAGAAATATGACTAT
ACAAATTTTTGCCGATGATAATGCTAAGGCGAGTGATTTTGTAAGACCAGGGCATATCAATCCTTTAATCGCAAAAAAAG
GTGGGGTTTTAGAACGCACAGGTCATACAGAAGGCACGGTTGATCTTTGTAAATTAGCAGGATTAAAAGGTGCTTGTGTG
ATTTGTGAAATTGTAAAAGATAATGGAGATATGGCAAGAAGAGAAGATCTAGAAATTTTTTGCCAAAAACATGATTTAAA
TATGATAGCCGTAAGCGATCTTATAGAATATCGCCTAAAACATGAAAGCCTTATTAAGCTTGAAGAAAAAAGTCAAAGTG
TTTTAGCAGGCTTTAAAGCAGAAAAATTTATCTTTAGTGATCATAATCAAACCCAACACATAGCTTTTTGCTTTAAAGAT
ATCAAAAAATGTGAAAATGTTAAATTTCATATCAGTGGTAGTGATTTTGAGCTTTTAACTTCAGATAAATTTTCAAAACT
TTTAGAGCAAATCAAATTTCTTAGTGAAAATGGTGGCGTTATAGTTTTCATGCAAGGAGAAAAATCCAGTACTACTCAAT
ACAAAAACTATGGTATAGGAGCACAGATTTTAAGATATTTTGGTATAGAAGAAATTAAACTTTTATCTCAAAGTTGTGAT
AAAGACTATATAGGTTTAGAAGGTTTTGGTTTAAATCTTAAAGCTTGTAATTTTAACTAA

Upstream 100 bases:

>100_bases
ATACCCCAATAAATATTTTTTAAAAGTGTGATTATAATGAATTTTAATTTATTTGTCAAGCAATTTAAGTTATAATTTTA
AAAATTTTAAGGAAATAAAA

Downstream 100 bases:

>100_bases
AAAGGAAAAAAATGACTTTAAAAGAACAAATTTTAAACGATATCAAAGAGGCAATGAAACAAAAAGATGATTTTAAAAGA
GATAGTTTAAGAACTCTAAA

Product: bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II protein

Products: NA

Alternate protein names: DHBP synthase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKFVSVEQAIKDLQAGKMLVMVDAEDRENEGDLIFPAQFSTQEKVNFMIKEARGVVCVALDETLAKKFELPLMVPKNTSN
HETAFTITVDAKDATTGVSAYERNMTIQIFADDNAKASDFVRPGHINPLIAKKGGVLERTGHTEGTVDLCKLAGLKGACV
ICEIVKDNGDMARREDLEIFCQKHDLNMIAVSDLIEYRLKHESLIKLEEKSQSVLAGFKAEKFIFSDHNQTQHIAFCFKD
IKKCENVKFHISGSDFELLTSDKFSKLLEQIKFLSENGGVIVFMQGEKSSTTQYKNYGIGAQILRYFGIEEIKLLSQSCD
KDYIGLEGFGLNLKACNFN

Sequences:

>Translated_339_residues
MKFVSVEQAIKDLQAGKMLVMVDAEDRENEGDLIFPAQFSTQEKVNFMIKEARGVVCVALDETLAKKFELPLMVPKNTSN
HETAFTITVDAKDATTGVSAYERNMTIQIFADDNAKASDFVRPGHINPLIAKKGGVLERTGHTEGTVDLCKLAGLKGACV
ICEIVKDNGDMARREDLEIFCQKHDLNMIAVSDLIEYRLKHESLIKLEEKSQSVLAGFKAEKFIFSDHNQTQHIAFCFKD
IKKCENVKFHISGSDFELLTSDKFSKLLEQIKFLSENGGVIVFMQGEKSSTTQYKNYGIGAQILRYFGIEEIKLLSQSCD
KDYIGLEGFGLNLKACNFN
>Mature_339_residues
MKFVSVEQAIKDLQAGKMLVMVDAEDRENEGDLIFPAQFSTQEKVNFMIKEARGVVCVALDETLAKKFELPLMVPKNTSN
HETAFTITVDAKDATTGVSAYERNMTIQIFADDNAKASDFVRPGHINPLIAKKGGVLERTGHTEGTVDLCKLAGLKGACV
ICEIVKDNGDMARREDLEIFCQKHDLNMIAVSDLIEYRLKHESLIKLEEKSQSVLAGFKAEKFIFSDHNQTQHIAFCFKD
IKKCENVKFHISGSDFELLTSDKFSKLLEQIKFLSENGGVIVFMQGEKSSTTQYKNYGIGAQILRYFGIEEIKLLSQSCD
KDYIGLEGFGLNLKACNFN

Specific function: Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family

Homologues:

Organism=Escherichia coli, GI1789420, Length=202, Percent_Identity=46.039603960396, Blast_Score=192, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6320695, Length=204, Percent_Identity=41.1764705882353, Blast_Score=166, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBB_CAMJE (Q9PHU4)

Other databases:

- EMBL:   AL111168
- PIR:   C81404
- RefSeq:   YP_002344002.1
- ProteinModelPortal:   Q9PHU4
- SMR:   Q9PHU4
- IntAct:   Q9PHU4
- GeneID:   904897
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj0572
- HOGENOM:   HBG735778
- OMA:   TSSHETA
- PhylomeDB:   Q9PHU4
- ProtClustDB:   PRK09314
- BioCyc:   CJEJ192222:CJ0572-MONOMER
- BRENDA:   4.1.99.12
- HAMAP:   MF_00180
- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299
- Gene3D:   G3DSA:3.90.870.10
- PIRSF:   PIRSF001259
- TIGRFAMs:   TIGR00506

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: =4.1.99.12

Molecular weight: Translated: 37925; Mature: 37925

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 32-32 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVSVEQAIKDLQAGKMLVMVDAEDRENEGDLIFPAQFSTQEKVNFMIKEARGVVCVAL
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHEECCCCEEEEEE
DETLAKKFELPLMVPKNTSNHETAFTITVDAKDATTGVSAYERNMTIQIFADDNAKASDF
CHHHHHHCCCCEEECCCCCCCCEEEEEEEECCCCCCCCHHHHCCCEEEEEECCCCCCCCC
VRPGHINPLIAKKGGVLERTGHTEGTVDLCKLAGLKGACVICEIVKDNGDMARREDLEIF
CCCCCCCEEEECCCCEEEECCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCHHCCHHEE
CQKHDLNMIAVSDLIEYRLKHESLIKLEEKSQSVLAGFKAEKFIFSDHNQTQHIAFCFKD
EECCCCCEEEHHHHHHHHHCHHHHEEEHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
IKKCENVKFHISGSDFELLTSDKFSKLLEQIKFLSENGGVIVFMQGEKSSTTQYKNYGIG
HHHHCCEEEEECCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHCCCCH
AQILRYFGIEEIKLLSQSCDKDYIGLEGFGLNLKACNFN
HHHHHHHCHHHHHHHHHHCCCCCCCCEECCCEEEEECCC
>Mature Secondary Structure
MKFVSVEQAIKDLQAGKMLVMVDAEDRENEGDLIFPAQFSTQEKVNFMIKEARGVVCVAL
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHEECCCCEEEEEE
DETLAKKFELPLMVPKNTSNHETAFTITVDAKDATTGVSAYERNMTIQIFADDNAKASDF
CHHHHHHCCCCEEECCCCCCCCEEEEEEEECCCCCCCCHHHHCCCEEEEEECCCCCCCCC
VRPGHINPLIAKKGGVLERTGHTEGTVDLCKLAGLKGACVICEIVKDNGDMARREDLEIF
CCCCCCCEEEECCCCEEEECCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCHHCCHHEE
CQKHDLNMIAVSDLIEYRLKHESLIKLEEKSQSVLAGFKAEKFIFSDHNQTQHIAFCFKD
EECCCCCEEEHHHHHHHHHCHHHHEEEHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
IKKCENVKFHISGSDFELLTSDKFSKLLEQIKFLSENGGVIVFMQGEKSSTTQYKNYGIG
HHHHCCEEEEECCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHCCCCH
AQILRYFGIEEIKLLSQSCDKDYIGLEGFGLNLKACNFN
HHHHHHHCHHHHHHHHHHCCCCCCCCEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10688204