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The map label for this gene is ligA [H]

Identifier: 57238218

GI number: 57238218

Start: 621674

End: 623617

Strand: Direct

Name: ligA [H]

Synonym: CJE0689

Alternate gene names: 57238218

Gene position: 621674-623617 (Clockwise)

Preceding gene: 57238219

Following gene: 57238217

Centisome position: 34.97

GC content: 34.21

Gene sequence:

>1944_bases
ATGAAAAAAGAAGAGTATTTAGAAAAAGTTGCTTTAGCTAATTTGTGGATGAGGGCATATTATGAAAAAGATGAACCCTT
GGCTAGCGATGAAGAATACGATGCTTTAATCAGAGAATTAAGAGTTTTTGAAGAACAAAATAAAGATGAAATTTCAAAAG
ACTCTCCAACGCAAAAAATAGCTCCTACTATACAAAGCGAGTTTAAAAAAATAGCTCATTTAAAGCGTATGTGGTCGATG
GAAGATGTGTTTGATGAGAGTGAGCTTAGAGCTTGGGCAAAACGCGCAAAATGTGAAAAAAACTTTTTTATAGAGCCTAA
ATTTGATGGAGCGAGTTTAAATTTGCTTTATGAAAATGGTAAATTAGTAAGCGGCGCCACTAGGGGCGATGGCGAAGTGG
GTGAGGATATAACGCTTAATGTTTTTGAGATAGAAAATATACCTAAAAATATAGCCTATAAAGAAAGGATAGAAATTCGT
GGAGAAGTGGTGATTTTAAAAGATGATTTTGAGAAAATCAACGAAAAAAGAGCCCTTTTAAATCAAAGTTTATTTGCCAA
TCCACGCAATGCTGCAAGTGGGAGTTTAAGGCAGCTTGATACAAGCATCACTAAAGAAAGAAATTTGAAATTTTATCCTT
GGGGAGTAGGGGAAAATACCTTAAATTTCACCAAACACAGCGAGGTGATGCAATTTATCAGAGAGCTTGGTTTTTTAAAG
GATGATTTTGTAAGACTTTGCGCTAATTTAGACGAAGTTTTAAAAGCTTATGATGAGCTTTTAGTTCTAAGAGAAAAAAA
ACCTATGATGATGGATGGAATGGTGGTAAGAGTTGATGATCTTGCACTTTGTGAAGAATTAGGCTATACGGTAAAATTTC
CAAAATTTATGGCAGCTTTTAAATTTCCTGCGCTTGAAAAAACCACGCGTTTAATAGGGGTAAATTTACAAGTAGGAAGA
AGTGGAGCGATCACTCCTGTGGCAGTTTTAGAACCTGTAAATTTAGATGGGGTTGTAGTAAAATCAGCCACCCTTCATAA
TTTTGATGAAATTGCAAGACTTGATGTTAAAATCAATGATTTTGTAAGCGTGATAAGAAGTGGCGATGTTATCCCTAAAA
TCACTAAGGTTTTTAAAGAGCGTAGAGAAGGTTTAGAGATGGAAATTTCACGCCCTAAGCTTTGTCCTACTTGCCAAAGT
GAGCTTTTGGATGAAGGAACTTTAATAAAATGTCAAAATATAGATTGTGAAGATAGACTTGTAAATTCTATCATTCATTT
TGTTTCTAAAAAATGTCTTAATATCGACGGACTGGGAGAAAATATAGTAGAACTTTTATACAAGCACAAAAAAATCACTA
CGCTTGAGAGTATTTTTCATTTAAAATTTAATGATTTTGAAGGTTTGGAAGGCTTTAAAGAAAAGAAAATTAACAATCTT
TTAAATGCCATAGAACAAGCAAGAGAATGTGAGCTTTTTCGTTTTATCACAGCTTTAGGAATAGAGCATATTGGCGAGGT
TGCGGCTAAAAAATTAAGTTTAAGTTTTGGTAAAGAGTGGTACAAGCAAAGCTTTGAAGCTTATGCTAATTTAGAAGGTT
TTGGAGAGCAAATGGCTTTAAGTCTTTGCGAATTTACGCGTGTTAATCATACAAGAATTGATGAGTTTTACAAGCTTTTA
AATTTGAAAATAGAAAAACTTGAAATCAAAAGCGATGGTGTGATTTTTGGTAAAACTTTTGTGATTACAGGCACTCTTTC
ACGCCCAAGAGATGAGTTTAAGGCTTTGATAGAAAAGCTTGGGGGTAAGGTAAGCGGCTCGGTTTCTAAAAAGACTGACT
ATGTGCTTTTTGGAGAAGAGGCAGGTTCTAAGCTTAGTAAGGCTAAAGAGCTTGAAGTAAAATGTATAGATGAAAGTGCT
TTTAATGAGCTTGTTAAAGAATAG

Upstream 100 bases:

>100_bases
GGCTTTTGAAAATGGTGCTAGTATTATAAGAGTGCATGATTTATATGAGCATAAACAACTTTTTGCTTTAGCGCAGGCAA
TGGATAATATAGGAGTTTAG

Downstream 100 bases:

>100_bases
TTCTTATATTTTAACGCTTTTAAGCCTTTTTGGCTTTTTGCTAACTTGGCAAAGGAGTGCTTTTTCTTTATTTTTTTTAA
TTCCTATTTTTTTAACACTT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKIAPTIQSEFKKIAHLKRMWSM
EDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENGKLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIR
GEVVILKDDFEKINEKRALLNQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLK
DDFVRLCANLDEVLKAYDELLVLREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAFKFPALEKTTRLIGVNLQVGR
SGAITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKINDFVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQS
ELLDEGTLIKCQNIDCEDRLVNSIIHFVSKKCLNIDGLGENIVELLYKHKKITTLESIFHLKFNDFEGLEGFKEKKINNL
LNAIEQARECELFRFITALGIEHIGEVAAKKLSLSFGKEWYKQSFEAYANLEGFGEQMALSLCEFTRVNHTRIDEFYKLL
NLKIEKLEIKSDGVIFGKTFVITGTLSRPRDEFKALIEKLGGKVSGSVSKKTDYVLFGEEAGSKLSKAKELEVKCIDESA
FNELVKE

Sequences:

>Translated_647_residues
MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKIAPTIQSEFKKIAHLKRMWSM
EDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENGKLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIR
GEVVILKDDFEKINEKRALLNQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLK
DDFVRLCANLDEVLKAYDELLVLREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAFKFPALEKTTRLIGVNLQVGR
SGAITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKINDFVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQS
ELLDEGTLIKCQNIDCEDRLVNSIIHFVSKKCLNIDGLGENIVELLYKHKKITTLESIFHLKFNDFEGLEGFKEKKINNL
LNAIEQARECELFRFITALGIEHIGEVAAKKLSLSFGKEWYKQSFEAYANLEGFGEQMALSLCEFTRVNHTRIDEFYKLL
NLKIEKLEIKSDGVIFGKTFVITGTLSRPRDEFKALIEKLGGKVSGSVSKKTDYVLFGEEAGSKLSKAKELEVKCIDESA
FNELVKE
>Mature_647_residues
MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKIAPTIQSEFKKIAHLKRMWSM
EDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENGKLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIR
GEVVILKDDFEKINEKRALLNQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLK
DDFVRLCANLDEVLKAYDELLVLREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAFKFPALEKTTRLIGVNLQVGR
SGAITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKINDFVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQS
ELLDEGTLIKCQNIDCEDRLVNSIIHFVSKKCLNIDGLGENIVELLYKHKKITTLESIFHLKFNDFEGLEGFKEKKINNL
LNAIEQARECELFRFITALGIEHIGEVAAKKLSLSFGKEWYKQSFEAYANLEGFGEQMALSLCEFTRVNHTRIDEFYKLL
NLKIEKLEIKSDGVIFGKTFVITGTLSRPRDEFKALIEKLGGKVSGSVSKKTDYVLFGEEAGSKLSKAKELEVKCIDESA
FNELVKE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=650, Percent_Identity=40.7692307692308, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI87082305, Length=540, Percent_Identity=21.8518518518519, Blast_Score=122, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73921; Mature: 73921

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHH
APTIQSEFKKIAHLKRMWSMEDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENG
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECC
KLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIRGEVVILKDDFEKINEKRALL
EEEECCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHEEECCCEEEEECCHHHHHHHHHHH
NQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLK
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCH
DDFVRLCANLDEVLKAYDELLVLREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAF
HHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHCCCCEECHHHHHHH
KFPALEKTTRLIGVNLQVGRSGAITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKIND
CCCCHHHHHHEEEEEEEECCCCCCCHHHEECCCCCCCEEEECHHHCCHHHHHHCCCCHHH
FVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQSELLDEGTLIKCQNIDCEDRL
HHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHCCCCCEEEECCCCHHHHH
VNSIIHFVSKKCLNIDGLGENIVELLYKHKKITTLESIFHLKFNDFEGLEGFKEKKINNL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LNAIEQARECELFRFITALGIEHIGEVAAKKLSLSFGKEWYKQSFEAYANLEGFGEQMAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
SLCEFTRVNHTRIDEFYKLLNLKIEKLEIKSDGVIFGKTFVITGTLSRPRDEFKALIEKL
HHHHHHHCCHHHHHHHHHHHCCEEEEEEECCCCEEEEEEEEEEECCCCCHHHHHHHHHHH
GGKVSGSVSKKTDYVLFGEEAGSKLSKAKELEVKCIDESAFNELVKE
CCCCCCCCCCCCCEEEECCHHCCHHHHHHCCEEEEECHHHHHHHHCC
>Mature Secondary Structure
MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHH
APTIQSEFKKIAHLKRMWSMEDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENG
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECC
KLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIRGEVVILKDDFEKINEKRALL
EEEECCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHEEECCCEEEEECCHHHHHHHHHHH
NQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLK
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCH
DDFVRLCANLDEVLKAYDELLVLREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAF
HHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHCCCCEECHHHHHHH
KFPALEKTTRLIGVNLQVGRSGAITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKIND
CCCCHHHHHHEEEEEEEECCCCCCCHHHEECCCCCCCEEEECHHHCCHHHHHHCCCCHHH
FVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQSELLDEGTLIKCQNIDCEDRL
HHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHCCCCCEEEECCCCHHHHH
VNSIIHFVSKKCLNIDGLGENIVELLYKHKKITTLESIFHLKFNDFEGLEGFKEKKINNL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LNAIEQARECELFRFITALGIEHIGEVAAKKLSLSFGKEWYKQSFEAYANLEGFGEQMAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
SLCEFTRVNHTRIDEFYKLLNLKIEKLEIKSDGVIFGKTFVITGTLSRPRDEFKALIEKL
HHHHHHHCCHHHHHHHHHHHCCEEEEEEECCCCEEEEEEEEEEECCCCCHHHHHHHHHHH
GGKVSGSVSKKTDYVLFGEEAGSKLSKAKELEVKCIDESAFNELVKE
CCCCCCCCCCCCCEEEECCHHCCHHHHHHCCEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA