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The map label for this gene is smpB [H]

Identifier: 57237987

GI number: 57237987

Start: 1165446

End: 1165898

Strand: Direct

Name: smpB [H]

Synonym: CJE1248

Alternate gene names: 57237987

Gene position: 1165446-1165898 (Clockwise)

Preceding gene: 57237986

Following gene: 57237988

Centisome position: 65.55

GC content: 30.46

Gene sequence:

>453_bases
ATGAAAATTATTGCGAGAAATAAAAAAGCTTTATTTGATTATAGCATCATAGAACGCTTTGAAGCCGGAATTGTCCTTAA
AGGAAGTGAAGTTGTAGCCTTAAGGGCAGGAAGAGCAAATCTTAAAGACTCTTTTGTTCGCATTATAAAAAATGAGATTT
TTTTACTTAATTCTCATATTTCATTACTTCATACTACACACTCTTTCTATAAACACGAAGAAAGAGGCGCTAGAAAGCTT
TTAATGCATAGAAAGCAAATTGATAAATTATTAGGTAAAGTAAGTATAGAAGGATATACTATCGTTGCATTAGATCTTTA
TTTTAACACAAAAAATAAAGTAAAAGCTACCCTTGCTTTAGCTAAAGGGAAAAATTTGCACGACAAGCGTGAAACTTTAA
AGAAAAAGCAAGCCGATTTAGAAGCTAAAGCTGCTATGAAAAATTACAAATAA

Upstream 100 bases:

>100_bases
TATTTACTCCTTGTGCAACTTTATATCCTAAAATGAAGTCTTACTTACAAGAAGATTTTTTTCTAAGCGGTAGCGGTAGT
AGTGTTTTTAAGGTTGATCG

Downstream 100 bases:

>100_bases
AGGAAAATAAATGAAAAAAATTTCAGCCCTATTTTTAATAAGTTTAGCATTTTTTCTTAATGCTTGCTCCAAGGAAGAAG
AAATTCAAAATGATTTTATG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKIIARNKKALFDYSIIERFEAGIVLKGSEVVALRAGRANLKDSFVRIIKNEIFLLNSHISLLHTTHSFYKHEERGARKL
LMHRKQIDKLLGKVSIEGYTIVALDLYFNTKNKVKATLALAKGKNLHDKRETLKKKQADLEAKAAMKNYK

Sequences:

>Translated_150_residues
MKIIARNKKALFDYSIIERFEAGIVLKGSEVVALRAGRANLKDSFVRIIKNEIFLLNSHISLLHTTHSFYKHEERGARKL
LMHRKQIDKLLGKVSIEGYTIVALDLYFNTKNKVKATLALAKGKNLHDKRETLKKKQADLEAKAAMKNYK
>Mature_150_residues
MKIIARNKKALFDYSIIERFEAGIVLKGSEVVALRAGRANLKDSFVRIIKNEIFLLNSHISLLHTTHSFYKHEERGARKL
LMHRKQIDKLLGKVSIEGYTIVALDLYFNTKNKVKATLALAKGKNLHDKRETLKKKQADLEAKAAMKNYK

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=146, Percent_Identity=42.4657534246575, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17193; Mature: 17193

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIARNKKALFDYSIIERFEAGIVLKGSEVVALRAGRANLKDSFVRIIKNEIFLLNSHI
CCEEECCCHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHEEEEECCH
SLLHTTHSFYKHEERGARKLLMHRKQIDKLLGKVSIEGYTIVALDLYFNTKNKVKATLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHEEEEEE
AKGKNLHDKRETLKKKQADLEAKAAMKNYK
CCCCCCCHHHHHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure
MKIIARNKKALFDYSIIERFEAGIVLKGSEVVALRAGRANLKDSFVRIIKNEIFLLNSHI
CCEEECCCHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHEEEEECCH
SLLHTTHSFYKHEERGARKLLMHRKQIDKLLGKVSIEGYTIVALDLYFNTKNKVKATLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHEEEEEE
AKGKNLHDKRETLKKKQADLEAKAAMKNYK
CCCCCCCHHHHHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA