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The map label for this gene is ispE [H]

Identifier: 57237986

GI number: 57237986

Start: 1164682

End: 1165449

Strand: Direct

Name: ispE [H]

Synonym: CJE1247

Alternate gene names: 57237986

Gene position: 1164682-1165449 (Clockwise)

Preceding gene: 57237985

Following gene: 57237987

Centisome position: 65.51

GC content: 28.26

Gene sequence:

>768_bases
ATGAAAGCTTACGCTAAAGCAAATATTTTTTTAAAACTTACCGGTTTTGACTCAAGAAAATATCATCTTTTAGAATCACG
ATTTATACTTTTAAAAGATGTTTTTGATGAGCTTGAGCTTGTTGACAAAGAATCAGATTCTAAAAAAGAATTTGAAATCA
TTAGTAATTTTAAATGCGAAAATAATATTATTCAAAAGGCTTATTTGCTACTTTCTAGAAGATATAATAACGAATTAAAA
GAATTATTTAGCAAAAAAAGTCTAAAACTCACAAAAAATATCCCAGTATGTGCTGGGCTTGGTGGTGGAAGTAGCGATTG
TGCGAGTTTTTTACTACTAATAAATGAAACCCTAAATTTAAAATTAAACCTACAAGAACTTATAAATTTAAGCATACAAC
TAGGAAGTGATATTGCCTTTTTTTTAAGTGGCTTTCACTCTGCTAATGTAAGTAGCTGTGGCGAAATTATAGAAGAATTT
GAAGATGACATACCTAATTTAAAATGGACTTTTCCACAAATATCTTGCCAAACTAAAGCTGTTTATGATGAATTTGATAG
AGGAATATTTGACTTTCAAAAAAACAATAATCAAGCGCAAATATACAAAAAACTCAGCACAAAAGAGTTGTTACAAAATT
TTAAAAACAAAGAATTAAATGATTTATTTACTCCTTGTGCAACTTTATATCCTAAAATGAAGTCTTACTTACAAGAAGAT
TTTTTTCTAAGCGGTAGCGGTAGTAGTGTTTTTAAGGTTGATCGATGA

Upstream 100 bases:

>100_bases
TACTTAGAAAAGAACTTGTTCAACAAGTAAAGGATGAAAATTTGCATTCTGTTGTTCAGAATGATATTAAACTTGATGAC
TTAAGCAAAAAACTAATCAA

Downstream 100 bases:

>100_bases
AAATTATTGCGAGAAATAAAAAAGCTTTATTTGATTATAGCATCATAGAACGCTTTGAAGCCGGAATTGTCCTTAAAGGA
AGTGAAGTTGTAGCCTTAAG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKAYAKANIFLKLTGFDSRKYHLLESRFILLKDVFDELELVDKESDSKKEFEIISNFKCENNIIQKAYLLLSRRYNNELK
ELFSKKSLKLTKNIPVCAGLGGGSSDCASFLLLINETLNLKLNLQELINLSIQLGSDIAFFLSGFHSANVSSCGEIIEEF
EDDIPNLKWTFPQISCQTKAVYDEFDRGIFDFQKNNNQAQIYKKLSTKELLQNFKNKELNDLFTPCATLYPKMKSYLQED
FFLSGSGSSVFKVDR

Sequences:

>Translated_255_residues
MKAYAKANIFLKLTGFDSRKYHLLESRFILLKDVFDELELVDKESDSKKEFEIISNFKCENNIIQKAYLLLSRRYNNELK
ELFSKKSLKLTKNIPVCAGLGGGSSDCASFLLLINETLNLKLNLQELINLSIQLGSDIAFFLSGFHSANVSSCGEIIEEF
EDDIPNLKWTFPQISCQTKAVYDEFDRGIFDFQKNNNQAQIYKKLSTKELLQNFKNKELNDLFTPCATLYPKMKSYLQED
FFLSGSGSSVFKVDR
>Mature_255_residues
MKAYAKANIFLKLTGFDSRKYHLLESRFILLKDVFDELELVDKESDSKKEFEIISNFKCENNIIQKAYLLLSRRYNNELK
ELFSKKSLKLTKNIPVCAGLGGGSSDCASFLLLINETLNLKLNLQELINLSIQLGSDIAFFLSGFHSANVSSCGEIIEEF
EDDIPNLKWTFPQISCQTKAVYDEFDRGIFDFQKNNNQAQIYKKLSTKELLQNFKNKELNDLFTPCATLYPKMKSYLQED
FFLSGSGSSVFKVDR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=182, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 29268; Mature: 29268

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYAKANIFLKLTGFDSRKYHLLESRFILLKDVFDELELVDKESDSKKEFEIISNFKCE
CCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHH
NNIIQKAYLLLSRRYNNELKELFSKKSLKLTKNIPVCAGLGGGSSDCASFLLLINETLNL
HHHHHHHHHHHHHHHCHHHHHHHHHCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHCCE
KLNLQELINLSIQLGSDIAFFLSGFHSANVSSCGEIIEEFEDDIPNLKWTFPQISCQTKA
EEEHHHHHCCHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHH
VYDEFDRGIFDFQKNNNQAQIYKKLSTKELLQNFKNKELNDLFTPCATLYPKMKSYLQED
HHHHHHCCCEEHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FFLSGSGSSVFKVDR
CEEECCCCCEEEECC
>Mature Secondary Structure
MKAYAKANIFLKLTGFDSRKYHLLESRFILLKDVFDELELVDKESDSKKEFEIISNFKCE
CCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHH
NNIIQKAYLLLSRRYNNELKELFSKKSLKLTKNIPVCAGLGGGSSDCASFLLLINETLNL
HHHHHHHHHHHHHHHCHHHHHHHHHCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHCCE
KLNLQELINLSIQLGSDIAFFLSGFHSANVSSCGEIIEEFEDDIPNLKWTFPQISCQTKA
EEEHHHHHCCHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHH
VYDEFDRGIFDFQKNNNQAQIYKKLSTKELLQNFKNKELNDLFTPCATLYPKMKSYLQED
HHHHHHCCCEEHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FFLSGSGSSVFKVDR
CEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA