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The map label for this gene is livK [C]

Identifier: 57237906

GI number: 57237906

Start: 1080150

End: 1081265

Strand: Reverse

Name: livK [C]

Synonym: CJE1163

Alternate gene names: 57237906

Gene position: 1081265-1080150 (Counterclockwise)

Preceding gene: 57237907

Following gene: 57237905

Centisome position: 60.82

GC content: 31.18

Gene sequence:

>1116_bases
ATGAAAAAACTAACTTTAACTTTAAGTGTTTTAACTATGGTAAATTGCTTATATGCTAAGGATATTAATATAGGAGTAGT
TTTACCTTTAACTGGAACTGTGGCAGCTTATGGGCAAGATGTTTTTAACGGAATTGAACTGGCTAATAAGCTACAGCCAA
AACTTTCAAATGGAGATGTTATTAAACTTATCACAATAGATACAAAAGGAGATAAGCTTGAAACTTCAAATGGAGTTAAT
CGTTTAATAGCAACAGATAAAGTCCTTGGGATTATTGGTGAAGCTACAACTCCAAATACCATTCAAGCTATTTCTATAGC
AGAAGAGAAAAAAATTCCCCTAATAGCTCCAGTGGCATCTGGAGATAAATTGCTAGATAAAAAGAAATATGCAAGTAGAG
TTTGTTTTAAAGATAGTTTTCAAGGAGATAAATTTGCTACTTATGTATCTAAGGATTTAGGACTTAAAAATGCTGTTATC
ATCATAGATCAAAGCAATGTTTATTCTTTAGGTTTGGCAAGAGCTTTTGAAAATTCTTTTAAGAATAATGGTGGGAAAAT
TATAAAAAAACTTGTTATTAATTCAGGAGATAAAGATTTTAGAGCTGTAGTTTCTCAACTTAAGAGTTTAAATCCTGATT
TTGTTTATATGCCTATTTATCATCCTGAAGCTGCTTTAATTGCGAGACAAGCAAGACAAGTTGGTTTTGATAAACTTTTA
GCAGCAGGAGATGGTGTTAATAATCAAACTTTTATAGATCTTGGCGGAAGTGCTGTTAATGGAGTAATTTTCACTGATAG
TTTTGATTATAACAATCCATCTACACAATTAGGAATAGATTTTGTTGTAGCTTATGAGAAAGTGAAAGGAACAAAAGAAC
TTCCAGCATTTTCGGCTATGGGGGCTGATGCGTATTTTGTAATGTTCAATGCTATGAATGCTTGCGTTGATAATCTTAGC
AGCGAATGTATTAATAGTAAAATTCATCAAACTAAAGATTTTCAAGCTGTTGGTGGTGTGATTAGCATAGATGAAAGCGG
TAATGCTATTCGTTCTGTTGTGATTAAAGAAATTCAAAATCAAAAACAAAATTATAAAACAATTATAAATCCATAA

Upstream 100 bases:

>100_bases
TTTGCAAGTAAAAGATCTTCTTGAAGAAGAACCTGCGCAAAAAACAAAACATAATATATTTCTAAAATAAAATGTAAAAA
CTATAATTAAAAGGAATACT

Downstream 100 bases:

>100_bases
TTACTAAAATAAAGGTAGAGAATGAAAAAAAGTTTAATTTTAGCAAGTATTCTAAGTTTAAGTTTAAGTGCTGCGGAAGT
CAAAATAGGAGTTGTTTTAC

Product: high affinity branched-chain amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDVIKLITIDTKGDKLETSNGVN
RLIATDKVLGIIGEATTPNTIQAISIAEEKKIPLIAPVASGDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLGLKNAVI
IIDQSNVYSLGLARAFENSFKNNGGKIIKKLVINSGDKDFRAVVSQLKSLNPDFVYMPIYHPEAALIARQARQVGFDKLL
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGIDFVVAYEKVKGTKELPAFSAMGADAYFVMFNAMNACVDNLS
SECINSKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQNQKQNYKTIINP

Sequences:

>Translated_371_residues
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDVIKLITIDTKGDKLETSNGVN
RLIATDKVLGIIGEATTPNTIQAISIAEEKKIPLIAPVASGDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLGLKNAVI
IIDQSNVYSLGLARAFENSFKNNGGKIIKKLVINSGDKDFRAVVSQLKSLNPDFVYMPIYHPEAALIARQARQVGFDKLL
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGIDFVVAYEKVKGTKELPAFSAMGADAYFVMFNAMNACVDNLS
SECINSKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQNQKQNYKTIINP
>Mature_371_residues
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDVIKLITIDTKGDKLETSNGVN
RLIATDKVLGIIGEATTPNTIQAISIAEEKKIPLIAPVASGDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLGLKNAVI
IIDQSNVYSLGLARAFENSFKNNGGKIIKKLVINSGDKDFRAVVSQLKSLNPDFVYMPIYHPEAALIARQARQVGFDKLL
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGIDFVVAYEKVKGTKELPAFSAMGADAYFVMFNAMNACVDNLS
SECINSKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQNQKQNYKTIINP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=353, Percent_Identity=26.9121813031161, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789867, Length=355, Percent_Identity=26.4788732394366, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40164; Mature: 40164

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDV
CCEEEEEHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCE
IKLITIDTKGDKLETSNGVNRLIATDKVLGIIGEATTPNTIQAISIAEEKKIPLIAPVAS
EEEEEEECCCCCEECCCCCHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCEEEECCC
GDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLGLKNAVIIIDQSNVYSLGLARAFENSF
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEHHHHHHHHHHHH
KNNGGKIIKKLVINSGDKDFRAVVSQLKSLNPDFVYMPIYHPEAALIARQARQVGFDKLL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCHHHHH
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGIDFVVAYEKVKGTKELPAFSAM
HCCCCCCCCEEEEECCCEECCEEEECCCCCCCCCCCCCEEEEEEEHHHCCCCCCCCHHHC
GADAYFVMFNAMNACVDNLSSECINSKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEECCCCHHHHHHHHHHHHH
QKQNYKTIINP
HHHCCCCCCCC
>Mature Secondary Structure
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDV
CCEEEEEHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCE
IKLITIDTKGDKLETSNGVNRLIATDKVLGIIGEATTPNTIQAISIAEEKKIPLIAPVAS
EEEEEEECCCCCEECCCCCHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCEEEECCC
GDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLGLKNAVIIIDQSNVYSLGLARAFENSF
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEHHHHHHHHHHHH
KNNGGKIIKKLVINSGDKDFRAVVSQLKSLNPDFVYMPIYHPEAALIARQARQVGFDKLL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCHHHHH
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGIDFVVAYEKVKGTKELPAFSAM
HCCCCCCCCEEEEECCCEECCEEEECCCCCCCCCCCCCEEEEEEEHHHCCCCCCCCHHHC
GADAYFVMFNAMNACVDNLSSECINSKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEECCCCHHHHHHHHHHHHH
QKQNYKTIINP
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]