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The map label for this gene is livJ [C]

Identifier: 57237905

GI number: 57237905

Start: 1079019

End: 1080128

Strand: Reverse

Name: livJ [C]

Synonym: CJE1162

Alternate gene names: 57237905

Gene position: 1080128-1079019 (Counterclockwise)

Preceding gene: 57237906

Following gene: 57237904

Centisome position: 60.76

GC content: 32.07

Gene sequence:

>1110_bases
ATGAAAAAAAGTTTAATTTTAGCAAGTATTCTAAGTTTAAGTTTAAGTGCTGCGGAAGTCAAAATAGGAGTTGTTTTACC
GCTTAGCGGAGCTACTGCAGCATATGGACAAAGTGCACTAGAAGGGATTAAACTTGCAAATTCAATGCAATCAACTTTAA
GTAATGGAGATAAGGTTTCTCTTGCTATTATTGATACTAAAGGTGATAAATTAGAATCTTCGAGTGGAGCAAATCGTTTA
GTTTCTCAAGATAAAGTTATAGGTCTTATAGGGGAAATGGTTACTGCTAATACATTGCAAGTTATGCGCGTAGCAGAAGA
TAATAAAATTCCATTGATCGCTCCTGCTGCAACTGGTGATAGATTGCTTGATAAAAAAATATATTCTTCTCGAGTATGTT
TTATGGACAGTTTTCAAGGTTCATCTTTAGCAAAATATGTTTTTTCAAAACTTAATTACAAAAGTGCTGTTATTGTAGTA
GATCAAAGCACTGACTATTCCTTAGGTTTAGCAAAAGCTTTTGAAAAACAGTACAAGTCTAATGGTGGTCAAATTCTTAG
AATTTTAAGAGTTAATTCAGGTGATAAAGATTTTAGAGCTATTGTAGCTCAAGTTAAAAGTTTAAATCCAGAATTTATTT
TTTTACCGCTTTATTATAGCGAAGCTTCTTTATTTGCAAGACAATCAAAACTCGCAGGTTTAAATATTCCTATGGGTTCT
GCAGATGGAGTTGCTGATCAAACTTTTATTAGTTTAGCTGGAGATGCAAGCGAGGGGTATATTTTCACAGATAGTTTTGA
TGCTAATAATCCAACTACAAAATTAAGCAAAGAATTTATCAGTGTTTATGAAAAAGCTAAAGGAACAAAAGAAGTTCCAA
ATTTTTCGGCTATGGGTGCTGATGCGTATTTTGTAATGCTAAATGCTATGAATGCTTGCGTTGAAAATCTTACTAGTAAG
TGTGTCAATGAAAAAATTCACCAAACTAAAAATTATCAAGGTGTTTCTGGTGTTATCAGTATTGATCAAACAGGAAATGC
AACTCGTTCTGTAGTGGTTAAAGAAATTAAAAATCAGAAGCAAAATTATAAAGATATTATCAATCCTTAA

Upstream 100 bases:

>100_bases
CGGTAATGCTATTCGTTCTGTTGTGATTAAAGAAATTCAAAATCAAAAACAAAATTATAAAACAATTATAAATCCATAAT
TACTAAAATAAAGGTAGAGA

Downstream 100 bases:

>100_bases
AGGAAAAATAGAAAGATTATGGATTCTACTTTATTTTTACAACAACTTGTTAATGGACTTAGTTTAGGCAGTATGTATGC
CCTTATTGCTGTTGGCTATA

Product: high affinity branched-chain amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKKSLILASILSLSLSAAEVKIGVVLPLSGATAAYGQSALEGIKLANSMQSTLSNGDKVSLAIIDTKGDKLESSSGANRL
VSQDKVIGLIGEMVTANTLQVMRVAEDNKIPLIAPAATGDRLLDKKIYSSRVCFMDSFQGSSLAKYVFSKLNYKSAVIVV
DQSTDYSLGLAKAFEKQYKSNGGQILRILRVNSGDKDFRAIVAQVKSLNPEFIFLPLYYSEASLFARQSKLAGLNIPMGS
ADGVADQTFISLAGDASEGYIFTDSFDANNPTTKLSKEFISVYEKAKGTKEVPNFSAMGADAYFVMLNAMNACVENLTSK
CVNEKIHQTKNYQGVSGVISIDQTGNATRSVVVKEIKNQKQNYKDIINP

Sequences:

>Translated_369_residues
MKKSLILASILSLSLSAAEVKIGVVLPLSGATAAYGQSALEGIKLANSMQSTLSNGDKVSLAIIDTKGDKLESSSGANRL
VSQDKVIGLIGEMVTANTLQVMRVAEDNKIPLIAPAATGDRLLDKKIYSSRVCFMDSFQGSSLAKYVFSKLNYKSAVIVV
DQSTDYSLGLAKAFEKQYKSNGGQILRILRVNSGDKDFRAIVAQVKSLNPEFIFLPLYYSEASLFARQSKLAGLNIPMGS
ADGVADQTFISLAGDASEGYIFTDSFDANNPTTKLSKEFISVYEKAKGTKEVPNFSAMGADAYFVMLNAMNACVENLTSK
CVNEKIHQTKNYQGVSGVISIDQTGNATRSVVVKEIKNQKQNYKDIINP
>Mature_369_residues
MKKSLILASILSLSLSAAEVKIGVVLPLSGATAAYGQSALEGIKLANSMQSTLSNGDKVSLAIIDTKGDKLESSSGANRL
VSQDKVIGLIGEMVTANTLQVMRVAEDNKIPLIAPAATGDRLLDKKIYSSRVCFMDSFQGSSLAKYVFSKLNYKSAVIVV
DQSTDYSLGLAKAFEKQYKSNGGQILRILRVNSGDKDFRAIVAQVKSLNPEFIFLPLYYSEASLFARQSKLAGLNIPMGS
ADGVADQTFISLAGDASEGYIFTDSFDANNPTTKLSKEFISVYEKAKGTKEVPNFSAMGADAYFVMLNAMNACVENLTSK
CVNEKIHQTKNYQGVSGVISIDQTGNATRSVVVKEIKNQKQNYKDIINP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=283, Percent_Identity=28.9752650176678, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789867, Length=267, Percent_Identity=28.4644194756554, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39746; Mature: 39746

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLILASILSLSLSAAEVKIGVVLPLSGATAAYGQSALEGIKLANSMQSTLSNGDKVS
CCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LAIIDTKGDKLESSSGANRLVSQDKVIGLIGEMVTANTLQVMRVAEDNKIPLIAPAATGD
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCC
RLLDKKIYSSRVCFMDSFQGSSLAKYVFSKLNYKSAVIVVDQSTDYSLGLAKAFEKQYKS
HHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHC
NGGQILRILRVNSGDKDFRAIVAQVKSLNPEFIFLPLYYSEASLFARQSKLAGLNIPMGS
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHCCCCCCCCCC
ADGVADQTFISLAGDASEGYIFTDSFDANNPTTKLSKEFISVYEKAKGTKEVPNFSAMGA
CCCCCCHHEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DAYFVMLNAMNACVENLTSKCVNEKIHQTKNYQGVSGVISIDQTGNATRSVVVKEIKNQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
QNYKDIINP
HHHHHCCCC
>Mature Secondary Structure
MKKSLILASILSLSLSAAEVKIGVVLPLSGATAAYGQSALEGIKLANSMQSTLSNGDKVS
CCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LAIIDTKGDKLESSSGANRLVSQDKVIGLIGEMVTANTLQVMRVAEDNKIPLIAPAATGD
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCC
RLLDKKIYSSRVCFMDSFQGSSLAKYVFSKLNYKSAVIVVDQSTDYSLGLAKAFEKQYKS
HHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHC
NGGQILRILRVNSGDKDFRAIVAQVKSLNPEFIFLPLYYSEASLFARQSKLAGLNIPMGS
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHCCCCCCCCCC
ADGVADQTFISLAGDASEGYIFTDSFDANNPTTKLSKEFISVYEKAKGTKEVPNFSAMGA
CCCCCCHHEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DAYFVMLNAMNACVENLTSKCVNEKIHQTKNYQGVSGVISIDQTGNATRSVVVKEIKNQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
QNYKDIINP
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]