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The map label for this gene is pheS

Identifier: 57237726

GI number: 57237726

Start: 916615

End: 917607

Strand: Reverse

Name: pheS

Synonym: CJE0976

Alternate gene names: 57237726

Gene position: 917607-916615 (Counterclockwise)

Preceding gene: 57237728

Following gene: 57237725

Centisome position: 51.61

GC content: 32.73

Gene sequence:

>993_bases
TTGCAAAATTTTATAGAACAGATTCAAAAATGTGAGAATTTAAATGATTTGGAAGCCATTAGGATTTCCGTTCTTGGAAA
AAAAGGTATTTTAACAGAAGGCTTTACAAAGCTTAAAGAGTTAGAAGATGAGGCGAAAAAAGAATTTGCTGCTAAATTAA
ATGCGCAAAAAGAAATATTTAATGAGGCTTATTTAGCCAAATTTAAAGATTTGGAAAATCTTGCTTTAGAAGAAAGAATG
AAACAAGATGCTTTAAATTTTAACTATTTTGATGAAAGTATTACAACAGGTGCCTTGCATCCTGTTATGAGCACCATGGA
TAAAATCATAGAATATTTTATAGCTTTGAATTTTAGCATAGAAAAAGGGCCTTTAATAGAAGATGACTTTCATAATTTTG
AAGCGTTAAATTTACCTAAATCTCACCCCGCAAGAGATATGCAAGATACCTTTTATTTTGATGATAAAAGGCTTTTAAGA
ACACAAACTTCGCCAGTGCAAATACGGACAATGCTTGCACAAAAACCTCCTATTAGGATGATAGCCCCTGGTGCTGTATT
TAGAAGAGATTTTGATATTACGCATACTCCTATGTTTCATCAAGTTGAAGGCCTTGTGGTGGAAGAAGGGCAAAAGGTTA
GCTTTGCAAATTTAAAAAGTGTATTGGAAGATTTTTTGCGTTATATGTTTGGAGATGTGAAAGTTCGTTTTCGTCCAAGT
TTTTTCCCATTTACAGAACCTTCTGCTGAAGTTGATATTTCTTGTGTATTTTGCAAAGGAAAGGGTTGTAGAGTTTGCAA
ACATACAGGTTGGCTTGAAGTTTTAGGATGTGGAATAGTTGATCCTAATGTTTATAATTTTGTTGGTTATGAAAATGTAA
GTGGTTATGCTTTTGGATTGGGAGTAGAACGTTTTGCAATGCTTTTGCATCAAATTCCAGATTTAAGATCTTTATTTGAA
GGTGATTTGAGATTATTGGAGCAGTTTAGATGA

Upstream 100 bases:

>100_bases
GTTTGATACAATTATATCTTTAAAGTTTGGTTTTTATGCTGAATTTAAAATAGTTTTTAATAGAATTAGAACTTGAAATA
TTTTATTTAAGGTAAGAAAA

Downstream 100 bases:

>100_bases
TAATTACTAAAAGTTGGTTGAATGATTGGTTAGAACTTGAAGAAATAAGTTCAGATAAAATAGCTAAAACTTTAAACTCT
ATAGGTATAGAAGTTGATAG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MQNFIEQIQKCENLNDLEAIRISVLGKKGILTEGFTKLKELEDEAKKEFAAKLNAQKEIFNEAYLAKFKDLENLALEERM
KQDALNFNYFDESITTGALHPVMSTMDKIIEYFIALNFSIEKGPLIEDDFHNFEALNLPKSHPARDMQDTFYFDDKRLLR
TQTSPVQIRTMLAQKPPIRMIAPGAVFRRDFDITHTPMFHQVEGLVVEEGQKVSFANLKSVLEDFLRYMFGDVKVRFRPS
FFPFTEPSAEVDISCVFCKGKGCRVCKHTGWLEVLGCGIVDPNVYNFVGYENVSGYAFGLGVERFAMLLHQIPDLRSLFE
GDLRLLEQFR

Sequences:

>Translated_330_residues
MQNFIEQIQKCENLNDLEAIRISVLGKKGILTEGFTKLKELEDEAKKEFAAKLNAQKEIFNEAYLAKFKDLENLALEERM
KQDALNFNYFDESITTGALHPVMSTMDKIIEYFIALNFSIEKGPLIEDDFHNFEALNLPKSHPARDMQDTFYFDDKRLLR
TQTSPVQIRTMLAQKPPIRMIAPGAVFRRDFDITHTPMFHQVEGLVVEEGQKVSFANLKSVLEDFLRYMFGDVKVRFRPS
FFPFTEPSAEVDISCVFCKGKGCRVCKHTGWLEVLGCGIVDPNVYNFVGYENVSGYAFGLGVERFAMLLHQIPDLRSLFE
GDLRLLEQFR
>Mature_330_residues
MQNFIEQIQKCENLNDLEAIRISVLGKKGILTEGFTKLKELEDEAKKEFAAKLNAQKEIFNEAYLAKFKDLENLALEERM
KQDALNFNYFDESITTGALHPVMSTMDKIIEYFIALNFSIEKGPLIEDDFHNFEALNLPKSHPARDMQDTFYFDDKRLLR
TQTSPVQIRTMLAQKPPIRMIAPGAVFRRDFDITHTPMFHQVEGLVVEEGQKVSFANLKSVLEDFLRYMFGDVKVRFRPS
FFPFTEPSAEVDISCVFCKGKGCRVCKHTGWLEVLGCGIVDPNVYNFVGYENVSGYAFGLGVERFAMLLHQIPDLRSLFE
GDLRLLEQFR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=274, Percent_Identity=29.1970802919708, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI4758340, Length=281, Percent_Identity=30.2491103202847, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788007, Length=326, Percent_Identity=50.920245398773, Blast_Score=324, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI32566635, Length=254, Percent_Identity=32.6771653543307, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32563657, Length=288, Percent_Identity=29.8611111111111, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=288, Percent_Identity=29.8611111111111, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=366, Percent_Identity=25.9562841530055, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=279, Percent_Identity=28.3154121863799, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18858079, Length=304, Percent_Identity=28.9473684210526, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17137424, Length=287, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_CAMJ8 (A8FLU6)

Other databases:

- EMBL:   CP000814
- RefSeq:   YP_001482410.1
- ProteinModelPortal:   A8FLU6
- SMR:   A8FLU6
- STRING:   A8FLU6
- GeneID:   5617078
- GenomeReviews:   CP000814_GR
- KEGG:   cju:C8J_0834
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   CJEJ407148:C8J_0834-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37987; Mature: 37987

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFIEQIQKCENLNDLEAIRISVLGKKGILTEGFTKLKELEDEAKKEFAAKLNAQKEIF
CCHHHHHHHHHCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEAYLAKFKDLENLALEERMKQDALNFNYFDESITTGALHPVMSTMDKIIEYFIALNFSI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
EKGPLIEDDFHNFEALNLPKSHPARDMQDTFYFDDKRLLRTQTSPVQIRTMLAQKPPIRM
CCCCCCCCCCCCCEEECCCCCCCCCCCCHHHEECCHHHHHCCCCCHHHHHHHHCCCCCEE
IAPGAVFRRDFDITHTPMFHQVEGLVVEEGQKVSFANLKSVLEDFLRYMFGDVKVRFRPS
ECCCHHHHCCCCCCCCCHHHHHCCEEECCCCEECHHHHHHHHHHHHHHHHCCEEEEEECC
FFPFTEPSAEVDISCVFCKGKGCRVCKHTGWLEVLGCGIVDPNVYNFVGYENVSGYAFGL
CCCCCCCCCCEEEEEEEECCCCCCCHHCCCHHHHHCCCCCCCCCEEECCCCCCCCEEECC
GVERFAMLLHQIPDLRSLFEGDLRLLEQFR
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNFIEQIQKCENLNDLEAIRISVLGKKGILTEGFTKLKELEDEAKKEFAAKLNAQKEIF
CCHHHHHHHHHCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEAYLAKFKDLENLALEERMKQDALNFNYFDESITTGALHPVMSTMDKIIEYFIALNFSI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
EKGPLIEDDFHNFEALNLPKSHPARDMQDTFYFDDKRLLRTQTSPVQIRTMLAQKPPIRM
CCCCCCCCCCCCCEEECCCCCCCCCCCCHHHEECCHHHHHCCCCCHHHHHHHHCCCCCEE
IAPGAVFRRDFDITHTPMFHQVEGLVVEEGQKVSFANLKSVLEDFLRYMFGDVKVRFRPS
ECCCHHHHCCCCCCCCCHHHHHCCEEECCCCEECHHHHHHHHHHHHHHHHCCEEEEEECC
FFPFTEPSAEVDISCVFCKGKGCRVCKHTGWLEVLGCGIVDPNVYNFVGYENVSGYAFGL
CCCCCCCCCCEEEEEEEECCCCCCCHHCCCHHHHHCCCCCCCCCEEECCCCCCCCEEECC
GVERFAMLLHQIPDLRSLFEGDLRLLEQFR
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA