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The map label for this gene is intA [H]

Identifier: 57237546

GI number: 57237546

Start: 498700

End: 499902

Strand: Direct

Name: intA [H]

Synonym: CJE0544

Alternate gene names: 57237546

Gene position: 498700-499902 (Clockwise)

Preceding gene: 57237545

Following gene: 57237564

Centisome position: 28.05

GC content: 24.85

Gene sequence:

>1203_bases
ATGCTAACTCAAAAAGATATAGACAATTTAGAAATAAAAGATAAAAGGTATATGATTAGCGTAGGAGAACCAAAAGAATT
ATATGTCCGAGTTAATCCAACAGGTAAAAAAGTTTTTTATTTAAGAGCTTCAAAATTTAAAAATTTTATAACAATAGGGG
AATGCCAAAAAGGTGTTTTAAATGTTACAAATGCAAGAGAAAAAGCAAAAGATCTTTTAAAATCAATGTATGATGGAAAA
TTTATCGGCAAAAATGATAAGGTTATGACACTTGAAAAAGCAAATTTTCTTTATGTTGATATAAAATCTAAAAAATTAAA
TTCAGCTACAATCAAAAAAGAACAGTCAATTTTTAAAAAATATATTATTCCAACTTTGGGACAAAAAGATATAAATGAAT
TGAAAAAAGATGATTTTCTACCTATTTATGATTTAATGCAGAAAAAAGGAATATACGAAACAATAAATAAAAATATATCT
TTGCTATGTAGGATATTTGAGATTAGTAGACAAAGAGGTGACTTAAAAACAGATATAATACTTCAATTGAAAGATTTAAA
GAAATTTTACAATGAAGCAAATCACAATAAAGTTAAACATTTTAAAGCTATAGTAGAAGAACAAGAAATAAAAAATATGT
TAGAATGTATGAAAGAATATAAAAATCATCCACGAACAAATACAACTATAATTAATGCAATTTATTTTACGCTTTTAACA
GCACAAAGAAGTAAAAATATTCGATTTGCTAAATGGAGTGATATTGACTTTGAAAACAATCTTTGGATTATAAAAGCAGA
TGAAATGAAAGTAAGATCTAATGGTGATAATATTATCCCTTTAAATAAATATGCTTTAAAGATACTAGATATACAAAGAA
TTTTAAATGGAGATAAAAAGTATATTTTCGCTAATAATAATGGAACTATTAGCGAGAATTTTGCTGTAAGATTTTTTAAA
TTTTATAATTTAGAGCACACTATACATGGATATCGTTCTACTTTTAGAAGTGTTTATACTAATAAAAGCAATGAGTTAAT
TCAGCAAGGTATTAGTAAGGATATAGCAGAAATGATATTACATCATATAAACGGTAATGAAATAGAAAGGGCTTACAACA
GAGCCAAGGCAATTGATTTAAGAGTAAAACTTATGCAATGGTATGGAAATTACTTAAACTCTCTTTGTGAGTTTTGCTTT
TAA

Upstream 100 bases:

>100_bases
GAAATTTTTTCTATTTTTTCTTATAATTCTGTAGCCATTTATGTAGCCAAAGTAAAAAAGCTAATTCTGGCTACAAAAAT
ACTAAAAAAAGGTTTTTTTA

Downstream 100 bases:

>100_bases
TGTCTTTTAGCTTTAAGCCATTTTTCTATTTCTTTTATTTCATATCTTATGGATTTTCCTATGCGAATGTAAGGTATTTT
ACCATCTTGTCTTAACTTAA

Product: phage integrase family site specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MLTQKDIDNLEIKDKRYMISVGEPKELYVRVNPTGKKVFYLRASKFKNFITIGECQKGVLNVTNAREKAKDLLKSMYDGK
FIGKNDKVMTLEKANFLYVDIKSKKLNSATIKKEQSIFKKYIIPTLGQKDINELKKDDFLPIYDLMQKKGIYETINKNIS
LLCRIFEISRQRGDLKTDIILQLKDLKKFYNEANHNKVKHFKAIVEEQEIKNMLECMKEYKNHPRTNTTIINAIYFTLLT
AQRSKNIRFAKWSDIDFENNLWIIKADEMKVRSNGDNIIPLNKYALKILDIQRILNGDKKYIFANNNGTISENFAVRFFK
FYNLEHTIHGYRSTFRSVYTNKSNELIQQGISKDIAEMILHHINGNEIERAYNRAKAIDLRVKLMQWYGNYLNSLCEFCF

Sequences:

>Translated_400_residues
MLTQKDIDNLEIKDKRYMISVGEPKELYVRVNPTGKKVFYLRASKFKNFITIGECQKGVLNVTNAREKAKDLLKSMYDGK
FIGKNDKVMTLEKANFLYVDIKSKKLNSATIKKEQSIFKKYIIPTLGQKDINELKKDDFLPIYDLMQKKGIYETINKNIS
LLCRIFEISRQRGDLKTDIILQLKDLKKFYNEANHNKVKHFKAIVEEQEIKNMLECMKEYKNHPRTNTTIINAIYFTLLT
AQRSKNIRFAKWSDIDFENNLWIIKADEMKVRSNGDNIIPLNKYALKILDIQRILNGDKKYIFANNNGTISENFAVRFFK
FYNLEHTIHGYRSTFRSVYTNKSNELIQQGISKDIAEMILHHINGNEIERAYNRAKAIDLRVKLMQWYGNYLNSLCEFCF
>Mature_400_residues
MLTQKDIDNLEIKDKRYMISVGEPKELYVRVNPTGKKVFYLRASKFKNFITIGECQKGVLNVTNAREKAKDLLKSMYDGK
FIGKNDKVMTLEKANFLYVDIKSKKLNSATIKKEQSIFKKYIIPTLGQKDINELKKDDFLPIYDLMQKKGIYETINKNIS
LLCRIFEISRQRGDLKTDIILQLKDLKKFYNEANHNKVKHFKAIVEEQEIKNMLECMKEYKNHPRTNTTIINAIYFTLLT
AQRSKNIRFAKWSDIDFENNLWIIKADEMKVRSNGDNIIPLNKYALKILDIQRILNGDKKYIFANNNGTISENFAVRFFK
FYNLEHTIHGYRSTFRSVYTNKSNELIQQGISKDIAEMILHHINGNEIERAYNRAKAIDLRVKLMQWYGNYLNSLCEFCF

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=286, Percent_Identity=25.5244755244755, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788690, Length=415, Percent_Identity=24.3373493975904, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47082; Mature: 47082

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQKDIDNLEIKDKRYMISVGEPKELYVRVNPTGKKVFYLRASKFKNFITIGECQKGVL
CCCCCCCCCCEEECCEEEEEECCCEEEEEEECCCCCEEEEEEHHHCCCEEEECCCCCCHH
NVTNAREKAKDLLKSMYDGKFIGKNDKVMTLEKANFLYVDIKSKKLNSATIKKEQSIFKK
HHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHH
YIIPTLGQKDINELKKDDFLPIYDLMQKKGIYETINKNISLLCRIFEISRQRGDLKTDII
HHCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHH
LQLKDLKKFYNEANHNKVKHFKAIVEEQEIKNMLECMKEYKNHPRTNTTIINAIYFTLLT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AQRSKNIRFAKWSDIDFENNLWIIKADEMKVRSNGDNIIPLNKYALKILDIQRILNGDKK
HHHCCCCEEEECCCCCCCCCEEEEEECCEEEECCCCCEEECHHHHHHHHHHHHHHCCCCE
YIFANNNGTISENFAVRFFKFYNLEHTIHGYRSTFRSVYTNKSNELIQQGISKDIAEMIL
EEEECCCCCCCCHHHEEEHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HHINGNEIERAYNRAKAIDLRVKLMQWYGNYLNSLCEFCF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTQKDIDNLEIKDKRYMISVGEPKELYVRVNPTGKKVFYLRASKFKNFITIGECQKGVL
CCCCCCCCCCEEECCEEEEEECCCEEEEEEECCCCCEEEEEEHHHCCCEEEECCCCCCHH
NVTNAREKAKDLLKSMYDGKFIGKNDKVMTLEKANFLYVDIKSKKLNSATIKKEQSIFKK
HHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHH
YIIPTLGQKDINELKKDDFLPIYDLMQKKGIYETINKNISLLCRIFEISRQRGDLKTDII
HHCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHH
LQLKDLKKFYNEANHNKVKHFKAIVEEQEIKNMLECMKEYKNHPRTNTTIINAIYFTLLT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AQRSKNIRFAKWSDIDFENNLWIIKADEMKVRSNGDNIIPLNKYALKILDIQRILNGDKK
HHHCCCCEEEECCCCCCCCCEEEEEECCEEEECCCCCEEECHHHHHHHHHHHHHHCCCCE
YIFANNNGTISENFAVRFFKFYNLEHTIHGYRSTFRSVYTNKSNELIQQGISKDIAEMIL
EEEECCCCCCCCHHHEEEHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HHINGNEIERAYNRAKAIDLRVKLMQWYGNYLNSLCEFCF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]