Click here to switch to the map view.

The map label for this gene is cofH [H]

Identifier: 57237516

GI number: 57237516

Start: 464655

End: 465701

Strand: Direct

Name: cofH [H]

Synonym: CJE0512

Alternate gene names: 57237516

Gene position: 464655-465701 (Clockwise)

Preceding gene: 57237514

Following gene: 57237517

Centisome position: 26.14

GC content: 34.1

Gene sequence:

>1047_bases
TTGAAAAGACTGGATAAAAAAGAAGCTTTAGATTTATTACATCATGCGAGTTTAACTGAACTTGGAGAAATGGCTTATAA
AAGAAAATTAGAACTTCATCCTGAAAAAATCACCACTTTTGTTGTAGATAGAAATATTAATTATACCAATGTTTGTTGTA
TTGATTGTTCTTTTTGTGCTTTTTATCGCCATCATAAAGAAGATGATGCTTATATTTCAAGCTTTGAGGAGATAGGCAAG
AAGATAGAGGAGCTTGAAGCTATTGGGGGTACGCAAATTCTTTTTCAAGGCGGGGTGCACCCAAAGTTAAAAATAGAATG
GTATGAAGAATTAGTTTCTTGGATAAAAGAACATTATCCAAATATCACTGTACATGGTTTTTCAGCGGTTGAGATCGCTT
ATATCGCAAAGGCATCTAAAATTTCAATTACTGAAGTACTTCAAAGACTACAAGCTAAAGGTTTATTTTCTATACCAGGA
GCAGGTGCTGAGGTGCTAAGCGATAGGGTGCGTGATATTATCGCACCCAATAAATGTGATACAGCTACTTGGTTAGAAGT
ACATCGCCAAGCTCATAAAATCGGCATGAAAAGTACAGCTACTATGATGTTTGGAACGGTTGAAAATGATGAAGAAATCA
TCGATCATTTTGAGCATTTGAGAAAATTGCAAGATGAAACAGGGGGATTTAGAGCTTTTATTTTATGGAGCTTTCAAAGT
GATAATACTGCTTTAATCCAAAAACATCCAGAGATTATGAAACAAAGTTCTAACAAGTATTTAAGACTTTTAGCTTTAGC
AAGACTTTATTTAGATAATTTTAAAAATTTACAAAGTTCTTGGGTAACTCAAGGCTCGCTTATAGGTCAACTTGCTTTAA
AATTTGGGGCCAATGATTTAGGTTCAACCATGATGGAAGAAAATGTAGTAAGTGCTGCAGGAGCAAGTTATAGAATGAAT
CAAGATGAGATGATAAGGCTGATTAGAAGTTTAGGCGAAAATCCAGCTAAACGCAATACCGCTTATGAAATTTTAGAAAG
GTTTTAA

Upstream 100 bases:

>100_bases
TTTTTTAATAGTTCAATGTAATTCATAAGCGAAACTATAGCATAATTTATATTTTAAATGCTATAATTATTTTAATTATG
TAAAAAAACAAGGAAAAATT

Downstream 100 bases:

>100_bases
TGCAGTATTTAGAGAGTAGGGGTATTAAAATCCCTTTTATTTTTGAAAAAAATTCTGATTTTCCTATTGTTGTTTTAAAG
CTTGTTTTTAGAAATTGTGC

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKRLDKKEALDLLHHASLTELGEMAYKRKLELHPEKITTFVVDRNINYTNVCCIDCSFCAFYRHHKEDDAYISSFEEIGK
KIEELEAIGGTQILFQGGVHPKLKIEWYEELVSWIKEHYPNITVHGFSAVEIAYIAKASKISITEVLQRLQAKGLFSIPG
AGAEVLSDRVRDIIAPNKCDTATWLEVHRQAHKIGMKSTATMMFGTVENDEEIIDHFEHLRKLQDETGGFRAFILWSFQS
DNTALIQKHPEIMKQSSNKYLRLLALARLYLDNFKNLQSSWVTQGSLIGQLALKFGANDLGSTMMEENVVSAAGASYRMN
QDEMIRLIRSLGENPAKRNTAYEILERF

Sequences:

>Translated_348_residues
MKRLDKKEALDLLHHASLTELGEMAYKRKLELHPEKITTFVVDRNINYTNVCCIDCSFCAFYRHHKEDDAYISSFEEIGK
KIEELEAIGGTQILFQGGVHPKLKIEWYEELVSWIKEHYPNITVHGFSAVEIAYIAKASKISITEVLQRLQAKGLFSIPG
AGAEVLSDRVRDIIAPNKCDTATWLEVHRQAHKIGMKSTATMMFGTVENDEEIIDHFEHLRKLQDETGGFRAFILWSFQS
DNTALIQKHPEIMKQSSNKYLRLLALARLYLDNFKNLQSSWVTQGSLIGQLALKFGANDLGSTMMEENVVSAAGASYRMN
QDEMIRLIRSLGENPAKRNTAYEILERF
>Mature_348_residues
MKRLDKKEALDLLHHASLTELGEMAYKRKLELHPEKITTFVVDRNINYTNVCCIDCSFCAFYRHHKEDDAYISSFEEIGK
KIEELEAIGGTQILFQGGVHPKLKIEWYEELVSWIKEHYPNITVHGFSAVEIAYIAKASKISITEVLQRLQAKGLFSIPG
AGAEVLSDRVRDIIAPNKCDTATWLEVHRQAHKIGMKSTATMMFGTVENDEEIIDHFEHLRKLQDETGGFRAFILWSFQS
DNTALIQKHPEIMKQSSNKYLRLLALARLYLDNFKNLQSSWVTQGSLIGQLALKFGANDLGSTMMEENVVSAAGASYRMN
QDEMIRLIRSLGENPAKRNTAYEILERF

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39705; Mature: 39705

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLDKKEALDLLHHASLTELGEMAYKRKLELHPEKITTFVVDRNINYTNVCCIDCSFCA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCCEEEEEEHHHHH
FYRHHKEDDAYISSFEEIGKKIEELEAIGGTQILFQGGVHPKLKIEWYEELVSWIKEHYP
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCC
NITVHGFSAVEIAYIAKASKISITEVLQRLQAKGLFSIPGAGAEVLSDRVRDIIAPNKCD
CEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TATWLEVHRQAHKIGMKSTATMMFGTVENDEEIIDHFEHLRKLQDETGGFRAFILWSFQS
HHHHHHHHHHHHHHCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC
DNTALIQKHPEIMKQSSNKYLRLLALARLYLDNFKNLQSSWVTQGSLIGQLALKFGANDL
CCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GSTMMEENVVSAAGASYRMNQDEMIRLIRSLGENPAKRNTAYEILERF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHC
>Mature Secondary Structure
MKRLDKKEALDLLHHASLTELGEMAYKRKLELHPEKITTFVVDRNINYTNVCCIDCSFCA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCCEEEEEEHHHHH
FYRHHKEDDAYISSFEEIGKKIEELEAIGGTQILFQGGVHPKLKIEWYEELVSWIKEHYP
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCC
NITVHGFSAVEIAYIAKASKISITEVLQRLQAKGLFSIPGAGAEVLSDRVRDIIAPNKCD
CEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TATWLEVHRQAHKIGMKSTATMMFGTVENDEEIIDHFEHLRKLQDETGGFRAFILWSFQS
HHHHHHHHHHHHHHCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC
DNTALIQKHPEIMKQSSNKYLRLLALARLYLDNFKNLQSSWVTQGSLIGQLALKFGANDL
CCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GSTMMEENVVSAAGASYRMNQDEMIRLIRSLGENPAKRNTAYEILERF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]